Öz
Aim: Pancreatic cancer is an important health problem with high mortality despite current treatment methods. Therefore, new methods for treatment are needed to be developed. Photodynamic therapy (PDT) is based on firstly pre-treatment of tumor region with a light-sensitive (PS) agent, following local accumulation in tumor loci irradiation with appropriate wavelength light source. The aim of this study is to determine the cytotoxic effects of chlorine e-6 based photodynamic therapy on MIA PaCa-2 pancreatic epithelial cancer cells and MRC-5 normal lung (non-cancerous) epithelial cells.
Methods: MIA PaCa-2 pancreatic epithelial cancer cells and MRC-5 normal epithelial cells were incubated with 10 μM Ce6 during 60 min and followed by irradiated with a diode laser (λ =670nm) at 5 J/cm2. Cell proliferation test was performed with WST-1 assay 4 - 24- 48 and 72 hours after post treatment.
Results: Ce6-based photodynamic therapy significantly reduced cell viability in the MIAPaCa-2 group compared to other treatment groups (p <0.05). On the other hand, it was determined that Ce6-based PDT was 33% cytotoxic on MRC-5 cells.
Conclusion: Ce6 concentration, incubation time and laser parameters should be determined separately for each cancer cell line. Ce6 based photodynamic therapy is promising as a palliative treatment option and / or minimizing tumor in pancreatic cancer.
Anahtar Kelimeler
Kaynakça
- 1. Greenlee R.T., Murray T., Bolden S. ve diğerleri, ''Cancer Statistics 2000''. CA Cancer J Clin., 2000;50:7–33.
- 2. DiMagno EP, Reber HA, Tempero MA. AGA Technical review on the epidemiology, diagnosis, and treatment of pancreatic ductal adenocarcinoma. Gastroenterology 1999; 11:1464–84.
- 3. Barr H, Tralau CJ, Boulos PB, MacRobert AJ, Tilly R, Bown SG. The contrasting mechanisms of colonic collagen damage between photodynamic therapy and thermal injury. Photochem Photobiol 1987;46: 795–800.
- 4. Agostinis P, Berg K, Cengel KA, Foster TH, Girotti AW, Gollnick SO, et al. Photodynamic therapy of cancer: an update. CA Cancer J Clin 2011;61:250-81.
- 5. O'Connor AE, Gallagher WM, Byrne AT. Porphyrin and nonporphyrin photosensitizers in oncology: preclinical and clinical advances in photodynamic therapy. Photochem Photobiol 2009;85:1053-74.
- 6. Hadjur C, Richard MJ, Parat MO, Favier A, Jardon P. Photodynamically induced cytotoxicity of hypericin dye on human fibroblast cell line MRC5. J Photochem Photobiol B 1995;27:139-46.
- 7. Hamid SA, Zimmermann W, et al. In vitro Study for Photodynamic Therapy using Fotolon® in Glioma Treatment. Proc. SPIE 9542, Medical Laser Applications and Laser-Tissue Interactions VII, 2015; pp. 95420B-95420B-13.
- 8. Li Y, Yu Y, Kang L, Lu Y. Effects of chlorin e6-mediated photodynamic therapy on human colon cancer SW480 cells. Int J Clin Exp Med 2014;7; 4867.
- 9. Li PT, Ke E S, Chiang P C, Tsai T. ALA-or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells. J Dent Sci 2015;10: 74-80.
- 10. Deng J, Long C, Hao F. Therapeutic effect of photodynamic therapy with chlorin e6 and 5-aminolevulinic acid on malignant melanoma in mice. Acta Academiae Medicinae Militaris Tertiae 2006;16:010.
Öz
Amaç: Pankreas kanseri, güncel tedavi yöntemlerine karşın halen yüksek mortalitesi olan önemli bir sağlık sorunudur. Bu yüzden yeni tedavi metotlarının geliştirilmesine ihtiyaç duyulmaktadır. Fotodinamik terapi (PDT), ışığa duyarlı (PS) bir ajanın önceden verilmesi ve lokal olarak tümör dokusunda birikmesi sonrasında ajanın absorpsiyon yapacağı dalga boyunda bir ışık kaynağı ile uyarılması esasına dayanır. Bu çalışmanın amacı MIAPaCa-2 pankreas epitelyal kanser hücreleri ile MRC-5 normal akciğer (kanserli olmayan) epitelyal hücreler üzerinde chlorine e-6 tabanlı fotodinamik terapinin sitotoksik etkisini belirlemektedir.
Yöntemler: MIAPaCa-2 pankreas epitelyal kanser hücreleri ile MRC-5 normal epitelyal hücreleri 10μM Ce6 ile 60 dakika boyunca inkübe edildikten sonra 670 nm dalgaboyuna sahip bir diyot lazer ile 5 J/cm2 ile uyarılmıştır. Tedaviden 4 – 24- 48 ve 72 saat sonra WST-1 ile hücre proliferasyonu testi yapılmıştır.
Bulgular: Ce6 tabanlı fotodinamik terapinin MIAPaCa-2 grubunda diğer tedavi gruplarına kıyasla hücre canlılığını anlamlı bir oranda azalttığı bulunmuştur (p<0.05). Diğer yandan Ce6 tabanlı PDT’nin MRC-5 hücreleri üzerinde %33 sitotoksik olduğu belirlenmiştir.
Sonuç: Işığa duyarlı ajan Ce6 konsantrasyonu, inkübasyon süresi ve lazer parametresi her hücre hattı için ayrı ayrı belirlenmelidir. Ce6 tabanlı fotodinamik tedavi pankreas kanserinde tümörü küçültmede ve/veya palyatif bir tedavi seçeneği olarak umut vaat etmektedir.
Anahtar Kelimeler
Kaynakça
- 1. Greenlee R.T., Murray T., Bolden S. ve diğerleri, ''Cancer Statistics 2000''. CA Cancer J Clin., 2000;50:7–33.
- 2. DiMagno EP, Reber HA, Tempero MA. AGA Technical review on the epidemiology, diagnosis, and treatment of pancreatic ductal adenocarcinoma. Gastroenterology 1999; 11:1464–84.
- 3. Barr H, Tralau CJ, Boulos PB, MacRobert AJ, Tilly R, Bown SG. The contrasting mechanisms of colonic collagen damage between photodynamic therapy and thermal injury. Photochem Photobiol 1987;46: 795–800.
- 4. Agostinis P, Berg K, Cengel KA, Foster TH, Girotti AW, Gollnick SO, et al. Photodynamic therapy of cancer: an update. CA Cancer J Clin 2011;61:250-81.
- 5. O'Connor AE, Gallagher WM, Byrne AT. Porphyrin and nonporphyrin photosensitizers in oncology: preclinical and clinical advances in photodynamic therapy. Photochem Photobiol 2009;85:1053-74.
- 6. Hadjur C, Richard MJ, Parat MO, Favier A, Jardon P. Photodynamically induced cytotoxicity of hypericin dye on human fibroblast cell line MRC5. J Photochem Photobiol B 1995;27:139-46.
- 7. Hamid SA, Zimmermann W, et al. In vitro Study for Photodynamic Therapy using Fotolon® in Glioma Treatment. Proc. SPIE 9542, Medical Laser Applications and Laser-Tissue Interactions VII, 2015; pp. 95420B-95420B-13.
- 8. Li Y, Yu Y, Kang L, Lu Y. Effects of chlorin e6-mediated photodynamic therapy on human colon cancer SW480 cells. Int J Clin Exp Med 2014;7; 4867.
- 9. Li PT, Ke E S, Chiang P C, Tsai T. ALA-or Ce6-PDT induced phenotypic change and suppressed migration in surviving cancer cells. J Dent Sci 2015;10: 74-80.
- 10. Deng J, Long C, Hao F. Therapeutic effect of photodynamic therapy with chlorin e6 and 5-aminolevulinic acid on malignant melanoma in mice. Acta Academiae Medicinae Militaris Tertiae 2006;16:010.
Ayrıntılar
| Konular | Klinik Tıp Bilimleri |
|---|---|
| Bölüm | Deneysel Çalışma |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Ağustos 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 2 Sayı: 2 |
