Öz
Heavy metal contamination is one of the major environmental hazards, leading to losses in agricultural yields and harmfully affecting human health. The most crucial function of plant cell is to respond against heavy metal stress for self-defence. The identification of the functional genes or proteins that are involved in responses to heavy-metal stress is a fundamental step in understanding the molecular mechanisms of stress responses. Protein expression is regulated not only at the transcriptional level, but also at the translational and post-translational levels. Therefore, it is crucial to investigate the changes in plant proteome under different stress conditions. Proteomics, defined as the large-scale analysis of proteins expressed by the genome, is not only a powerful molecular tool for describing complete proteome, but also for comparative proteomes under different physiological conditions, such as heavy metal stress. In this review, proteomics technologies, and heavy metal induced proteins which play a role in different metabolic events in plants are discussed. © Afyon Kocatepe Üniversitesi
Anahtar Kelimeler
Kaynakça
- Agrawal, G.K., Jwa, N.S., Shibato, J., Han, O., Iwahashi, H. and Rakwal, R., 2003. Diverse environmental cues transiently regulate OsOPR1 of the “octadecanoid pathway” revealing defense/stress and development. Biochemical and its Biophysical Research Communications, 310, 1073- 1082. importance in rice
- Ahsan, N., Lee, D.G., Lee, S.H., Kang, K. Y., Lee, J.J., Kim, P.J., Yoon, H.S., Kim, J.S. and Lee, B.Y., 2007. Excess copper induced physiological and proteomic changes in germinating rice seeds. Chemosphere, 67, 1182- 1193.
- Ahsan, N., Lee, D.G., Alam, I., Kim, P.J., Lee, J.J., Ahn, Y.O., Kwak, S.S., Lee, I.J., Bahk, J.D., Kang, K.Y., Renaut, J., Komatsu, S. and Lee, B.H., 2008. Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress. Proteomics, 8, 3561-3576.
- Ahsan, N., Renaut, J. and Komatsu, S., 2009. Recent developments in the application of proteomics to the analysis of plant responses to heavy metals. Proteomics, 9, 2602-2621.
- Ahsan, N. and Komatsu, S., 2009. Comparative analyses of the proteomes of leaves and flowers at various stages of development reveal organ-specific functional differentiation of proteins in soybean. Proteomics, 9, 4889-4907.
- Ahsan, N., Lee, D.G., Kim, K.H., Alam, I., Lee, S.H., Lee, K.W., Lee, H. and Lee, B.H., 2010. Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by two-dimensional gel electrophoresis
Öz
Ağır metal kirliliği, insan sağlığını olumsuz etkileyen ve tarımsal verimde kayıplara neden olan önemli çevresel tehlikelerden biridir. Bitki hücresinin en önemli fonksiyonu, kendi savunması için ağır metal stresine karşı cevap vermektir. Ağır metal stresine cevap olarak fonksiyonel genler veya proteinlerin teşhisi stres cevaplarının moleküler mekanizmalarının anlaşılmasında önemli bir basamaktır. Protein ekspresyonu sadece transkripsiyonel seviyede değil, aynı zamanda translasyonel ve post-translasyonel seviyelerde de düzenlenmektedir. Bu nedenle, farklı stres koşulları altında bitki proteomundaki değişimleri araştırmak önemlidir. Genom tarafından eksprese olan proteinlerin geniş ölçekli analizi olarak tanımlanan proteomik, sadece proteomun tamamını tanımlamak için değil, aynı zamanda ağır metal stresi gibi farklı fizyolojik koşullar altındaki proteomların karşılaştırılması için güçlü bir moleküler araçtır. Bu derlemede, proteomik teknolojileri ve bitkilerde farklı metabolik olaylarda rolü olan ağır metal teşvikli proteinler tartışılmıştır.
Anahtar Kelimeler
Bitkiler Ağır Metal Toksisite Proteomik Kütle Spektrometrisi
Kaynakça
- Agrawal, G.K., Jwa, N.S., Shibato, J., Han, O., Iwahashi, H. and Rakwal, R., 2003. Diverse environmental cues transiently regulate OsOPR1 of the “octadecanoid pathway” revealing defense/stress and development. Biochemical and its Biophysical Research Communications, 310, 1073- 1082. importance in rice
- Ahsan, N., Lee, D.G., Lee, S.H., Kang, K. Y., Lee, J.J., Kim, P.J., Yoon, H.S., Kim, J.S. and Lee, B.Y., 2007. Excess copper induced physiological and proteomic changes in germinating rice seeds. Chemosphere, 67, 1182- 1193.
- Ahsan, N., Lee, D.G., Alam, I., Kim, P.J., Lee, J.J., Ahn, Y.O., Kwak, S.S., Lee, I.J., Bahk, J.D., Kang, K.Y., Renaut, J., Komatsu, S. and Lee, B.H., 2008. Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress. Proteomics, 8, 3561-3576.
- Ahsan, N., Renaut, J. and Komatsu, S., 2009. Recent developments in the application of proteomics to the analysis of plant responses to heavy metals. Proteomics, 9, 2602-2621.
- Ahsan, N. and Komatsu, S., 2009. Comparative analyses of the proteomes of leaves and flowers at various stages of development reveal organ-specific functional differentiation of proteins in soybean. Proteomics, 9, 4889-4907.
- Ahsan, N., Lee, D.G., Kim, K.H., Alam, I., Lee, S.H., Lee, K.W., Lee, H. and Lee, B.H., 2010. Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by two-dimensional gel electrophoresis
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Ağustos 2013 |
| Gönderilme Tarihi | 8 Ağustos 2015 |
| Yayımlandığı Sayı | Yıl 2013 Cilt: 13 Sayı: 2 |