Intra-Genetic Variation within Olive Cultivar 'Nabali' in Palestine by Microsatellite and Random Amplified Polymorphic DNA

Yıl 2018, Cilt: 28 Sayı: 1, 30 - 36, 15.05.2018

Aziz Salameh Soha Gezaeıl Alaa Lahlooh Dina Arafat

Öz

ABSTRACT:
Over seventy olive trees (Nabali) from different regions in Palestine were used
in this study. Intra-genetic variation within different olive Nabali variants
were approved by SSR and RAPD markers. Four SSRs bands were monomorphic
revealing a true-to-genotype of Nabali cultivar. Ten RAPD markers produced 60
reproducible bands with an average of 6 bands / marker. Only 24 were
polymorphic. The percentage of polymorphic bands was 38% which is relatively
high. Similarity matrix for studied populations ranged from moderate (0.610)
for Jalkamous and Karawa Bani Zaid (2) to highly genetic similarity or even
identity (1.000) in some cases as Aqraba and Aseerah (N).  The interaction between different variants
trends to be high. The effect of geographic location was absent in this study
and has no significant contribution. Dendogram based on Jaccards coffiecnt  revealed three main clusters, the biggest
group consisted of the majority of variants including Bieta, Karawa Bani Zaid
(1), Salfeet, Salfeet (h), Aqraba, Aseereh (N), Jalkamous, and Alaroub. Second
group consisted of two variants Alaar and Nahaleen. The third group is
containing only Karawa Bani Zaid (2). The relative high polymorphic bands of
RAPD markers (40%) and moderate genetic similarity among different Nabali
variants suggested attribution of genetic background. Selection for new traits
within Nabali is suggested.

 

Anahtar Kelimeler

Olive, Intra-Genetic Variation, SSR, RAPD.

Intra-Genetic Variation within Olive Cultivar 'Nabali' in Palestine by Microsatellite and Random Amplified Polymorphic DNA

Öz

ABSTRACT:
Over seventy olive trees (Nabali) from different regions in Palestine were used
in this study. Intra-genetic variation within different olive Nabali variants
were approved by SSR and RAPD markers. Four SSRs bands were monomorphic
revealing a true-to-genotype of Nabali cultivar. Ten RAPD markers produced 60
reproducible bands with an average of 6 bands / marker. Only 24 were
polymorphic. The percentage of polymorphic bands was 38% which is relatively
high. Similarity matrix for studied populations ranged from moderate (0.610)
for Jalkamous and Karawa Bani Zaid (2) to highly genetic similarity or even
identity (1.000) in some cases as Aqraba and Aseerah (N).  The interaction between different variants
trends to be high. The effect of geographic location was absent in this study
and has no significant contribution. Dendogram based on Jaccards coffiecnt  revealed three main clusters, the biggest
group consisted of the majority of variants including Bieta, Karawa Bani Zaid
(1), Salfeet, Salfeet (h), Aqraba, Aseereh (N), Jalkamous, and Alaroub. Second
group consisted of two variants Alaar and Nahaleen. The third group is
containing only Karawa Bani Zaid (2). The relative high polymorphic bands of
RAPD markers (40%) and moderate genetic similarity among different Nabali
variants suggested attribution of genetic background. Selection for new traits
within Nabali is suggested.

 

Anahtar Kelimeler

Olive, Intra-Genetic Variation, SSR, RAPD.

Kaynakça

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