Can E12 sheet plastination be used to examine the presence and incidence of intra-articular spinal meniscoids?

Yıl 2015, Cilt: 9 Sayı: 1, 13 - 18, 20.06.2015

Scott F. Farrell Peter G. Osmotherly Darren A. Rivett Jon Cornwall

https://doi.org/10.2399/ana.14.046

Öz

Objectives: The role of intra-articular meniscoids in spinal pathology is unclear, and their anatomy not well understood. Spinal meniscoids have been identified in research using dissection and imaging. No previous studies have identified these structures using E12 sheet plastination; such methods of visualising meniscoids are likely of value in research and education. Our aim was to examine the presence of spinal meniscoids in cadaver sections that had undergone E12 sheet plastination.

Methods: The lateral atlantoaxial and cervical, thoracic and lumbar zygapophyseal joints were inspected in two female cadavers (65, 86 years) that had undergone E12 sheet plastination in 2 mm sagittal and transverse sections. Each slice was photographed on a light-box then inspected under magnification to identify individual joints, then each joint assessed for the presence of meniscoids.

Results: From 281 photographs, 92 zygapophyseal and four lateral atlantoaxial joints were identified. In sagittal sections, 24 meniscoids were identified in atlantoaxial and cervical joints and 32 meniscoids in the thoracic spine. In transverse sections, 14 meniscoids were identified in the thoracic spine and 12 meniscoids in the lumbar spine. Meniscoids were identified in all atlantoaxial and cervical joints, 78.9% of thoracic joints, and 80% of lumbar joints.

Conclusion: Spinal meniscoids were effectively visualised on E12 sheet plastinates in all spinal regions. Meniscoid frequency was similar to previous reports for the atlantoaxial and cervical joints, and higher for thoracic and lumbar joints. Sheet plastinations can therefore be utilised in research and education exploring the anatomy and clinical significance of the meniscoids.

Anahtar Kelimeler

E12 sheet plastination, meniscoid, spinal meniscoid, spine

Kaynakça

• Mercer S, Bogduk N. Intra-articular inclusions of the cervical syn- ovial joints. J Rheumatol 1993;32:705–10.
• Webb A, Collins P, Rassoulian H, Mitchell B. Synovial folds – a pain in the neck? Man Ther 2011;16:118–24.
• Farrell SF, Osmotherly PG, Cornwall J, Rivett DA. The anatomy and morphometry of cervical zygapophyseal joint meniscoids. Surg Radiol Anat 2014; DOI 10.1007/s00276–014–1406-3
• Farrell SF, Osmotherly PG, Cornwall J, Rivett DA. Morphology and morphometry of the atlantoaxial joint meniscoids. Anat Sci Int 2015; DOI 10.1007/s12565–015–0276-z
• Bogduk N, Engel R. The menisci of the lumbar zygapophyseal joints. J Anat 1982;135:795–809.
• Webb A, Darekar A, Sampson M, Rassoulian H. Synovial folds of the lateral atlantoaxial joints: in vivo quantitative assessment using magnetic resonance imaging in healthy volunteers. Spine (Phila Pa 1976) 2009;34:E697–702.
• Bogduk N, Engel R The menisci of the lumbar zygapophyseal joints. A review of their anatomy and significance. Spine (Phila Pa 1976) 1984;9:454–60.
• Friedrich KM, Reiter G, Pretterkleiber ML, Pinker K, Friedrich M, Trattnig S, Salomonowitz E. Reference data for in vivo mag- netic resonance imaging properties of meniscoids in the cervical zygapophyseal joints. Spine (Phila Pa 1976) 2008;33:e778–83.
• Bogduk N, Jull G. The theoretical pathology of acute locked back: a basis for manipulative therapy. Man Med 1985;1:78–82.
• Bogduk N. Clinical anatomy of the lumbar spine and sacrum. 4th ed. Edinburgh: Elsevier; 2005.
• Schonstrom N, Twomey L, Taylor J. The lateral atlantoaxial joints and their synovial folds: an in vitro study of soft tissue injuries and fractures. J Trauma 1993;35:886–92.
• Taylor J, Taylor M. Cervical spine injuries: an autopsy of 109 blunt injuries. J Musculoskelet Pain 1996;4:61–79.
• Bogduk N. On cervical zygapophyseal joint pain after whiplash. Spine (Phila Pa 1976) 2011;36:S194–9.
• von Hagens G, Tiedemann K, Kriz W. The current potential of plastination. Anat Embryol 1987;175:411–21.
• Cornwall J. The diverse utility of wet prosections and plastinated specimens in teaching gross anatomy in New Zealand. Anat Sci Educ 2011;4:269–74.
• Sora MC, Cook P. Epoxy plastination of biological tissue: E12 technique. Journal of Plastination 2007;22:31–9.
• Sora MC. Epoxy plastination of biological tissue: E12 ultra-thin technique. Journal of Plastination 2007;22:40–5.
• Phillips MN, Nash LG, Barnett RJ, Nicholson HD, Zhang M. The use of confocal microscopy for the examination of E12 sheet plastinated human tissue. Journal of Plastination 2002;17:12–6.
• Nash LG, Nicholson HD, Lee AS, Johnson GM, Zhang M. Configuration of the connective tissue in the posterior atlanto- occipital interspace: a sheet plastination and confocal microscopy study. Spine (Phila Pa 1976) 2005;17:1359–66.
• Johnson GM, Zhang M. Regional differences within the human supraspinous and interspinous ligaments: a sheet plastination study. Eur Spine J 2002;11:382–8.
• Inami S, Kaneoka K, Hayashi K, Ochiai N. Types of synovial fold in the cervical facet joint. J Orthop Sci 2000;5:475–80.
• Schulte T, FIiller T, Struwe P, Liem D, Bullman V. Intra-articu- lar meniscoid folds in thoracic zygapophyseal joints. Spine (Phila Pa 1976) 2010;E191–7.
• Brown M, Reed R, Henry R. Effects of dehydration mediums and temperature on total dehydration time and tissue shrinkage. Journal of Plastination 2002;17:28–33.
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• be used to examine the presence and incidence of intra-articular spinal meniscoids? Anatomy 2015;9(1):13–18.