A COMPARATIVE STUDY ON THE EMBRYO-FETAL DEVELOPMENT IN RABBITS AFTER ARTIFICIAL INSEMINATION

Yıl 2016, Cilt: 10 Sayı: 2, 105 - 113, 25.08.2016

Kosisochukwu Kingsley Obasi Ezra Lambe İsmail Temitayo Gbadamosi Adeoye Oyetunji Oyewopo

Öz

Objectives: Artificial insemination (AI) is the introduction of spermatozoa into a female's uterus or cervix for the purpose of achieving pregnancy. It is a fertility treatment for humans, in animal breeding and embryo-fetal developmental studies. This study investigated the embryo-fetal development in the rabbit via artificial insemination by evaluating the litter size, reproductive data and external morphological abnormalities after insemination.
Methods: Ten adult female and three male rabbits weighing 1.8 kg and 2 kg, respectively were used. Group A served as
the natural mating, while Group B served as the AI group, and the males served as semen donors. On day 28 of gestation, does were subjected to caesarean section. The ovaries and uteri were removed and examined for the number of corpora lutea and the status of all implantation.
Results: Results showed a significant increase in fetal weight, total fetal number, total resorption sites, number of implantation and total number of live foetuses (p<0.05), while there was no significant increase in parameters like average fetal weight, placenta weight, uterine gravid weight, number of corpora lutea and sex ratio in Group B compared to Group A (p>0.05). A conception rate of 100% was observed in Group A compared to Group B with 80% conception rate. External examination carried out on the foetuses revealed no external abnormalities in both groups.
Conclusion: The results of this study show the clear advantages in the use of rabbits for AI in terms of embryo-fetal developmental studies.

Anahtar Kelimeler

artificial insemination, caesarean section, embryo-fetal development

Kaynakça

• Theau Clément M. Advances in biostimulation methods applied to
• rabbit reproduction. World Rabbit Science 2000;8:61–79.
• Ombelet W, Van Robays J. Artificial insemination history: hurdles
• and milestones. Facts Views Vis Obgyn 2015;7:137–43.
• Carluccio A, Robbe D, De Amicis I, Contri A, Tosi U, Russo F,
• Paoletti M. Artificial insemination in rabbits: laboratory and field
• trial with three different semen extenders. World Rabbit Science
• ;12:65–79.
• Orji BI, Igboeli G, Nwakalor LN. Semen characteristics of Muturu
• bulls – Bos brachyceros. Theriogenology 1984;22:197–203.
• Barje PP, Osinowo OA, Dim NI. Effects of diluents, storage and
• depth of insemination on the fertility of chilled ram spermatozoa.
• Nigerian Journal of Animal Production 1994;21:42–6.
• Dede TI, Steinbach J. Artificial insemination of pigs in Nigeria: a
• preliminary report. Nigerian Journal of Animal Production 1977;4:101–8.
• Cherms FL. Variations in semen quality and relationship of semen
• quality to fertility in turkeys. Poult Sci 1968;47:746–54.
• Rose ID. Semen characteristics, fertility and hatchability in some
• breeds of cocks. MSc Thesis, Abubakar Tafawa Balewa University, Bauchi, Nigeria, 2000. p. 24–7.
• Quintela LA, Peña AI, Vega MD, Gullón J, Prieto MC, Barrio M,
• Becerra JJ, Maseda F, Herrdón PG. Ovulation induction in rabbit
• does submitted to artificial insemination by adding buserelin to the
• seminal dose. Reprod Nutr Dev 2004;44:79–88.
• Morrell JM. Artificial insemination in rabbits. Br Vet J 1995;151:477–88.
• Walton A. The relation between “density” of sperm-suspension and
• fertility: as determined by artificial insemination of rabbits. Proc Roy
• Soc Lond B 1927;101–303.
• Boussit D. Reproduction et insémination artificielle en cuniculture.
• Lempdes (France): Association Fran.aise de Cuniculture, INRA;
• p. 234.
• Mroueh A, Mastroianni L Jr. Insemination via the intraperitoneal
• route in rabbits. Fert Steril 1966;17:76–82.
• Kim JC, Chung MK, Han SS. An artificial insemination technique
• for obtaining pregnant does in rabbit teratogenicity studies. Korean
• Journal of Laboratory Animal Science 1996;12:141–6.
• Wise LD, Beck SL, Beltrame D, Beyer BK, Chahoud I, Clark RL,
• Clark R, Druga AM, Feuston MH, Guittin P, Henwood SM, Kimmel CA, Lindstrom P, Palmer AK, Petrere JA, Solomon HM, Yasuda M, York RG. Terminology of developmental abnormalities in common laboratory mammals (version 1). Teratology 1997;55:249–92.
• Drury RAB, Wallington EA. Preparation and fixation of tissues. In:
• Drury RAB, Wallington EA, editors. Carleton’s histological technique.
• th ed. Oxford: Oxford University Press; 1980. pp. 41–54.
• Rommers JM, Meijerhoft R, Noordhuizen JP, Kemp B.
• Relationships between body weight at first mating and subsequent
• body development, feed intake, and reproductive performance of
• rabbit does. J Anim Sci 2002;80:2036–42.
• Tawfeek MI, El-Gaafary MN. Evaluation of A.I. technique as compared to natural mating in association with some productive and
• reproductive traits in rabbits. Egyptian Journal of Rabbit Science 1991;1:13–20.
• Oke UK, Iheanacho VC. Effect of breed and breeding system on
• reproductive performance of rabbits in a humid tropical environment.
• Tropical and Subtropical Agroecosystems 2011;14:369–73.
• Khalifa RM. Mating frequency and artificial insemination in rabbits
• using vasectomized buck. World Rabbit Science 1994;2:1–5.
• Kermabon AY, Belair L, Theau-Clément M, Salesse R, Djiane J.
• Effect of anoestrus and bromocryptine treatment on the expression
• of prolactin and LH receptors in the rabbit ovary during lactation. J
• Reprod Fertil 1994;102:131–8.
• Rebollar PG, Ubilla E, Alvariño JMR, Illera JC, Silvan G. Effect of
• the level of sexual receptivity on plasma estradiol and the ovulatory
• response during the postpartum period in the rabbit. [Article in Spanish] Rev Esp Fisiol 1992;48:13–7.
• Hong JS, Yu WJ, Kim YS, Cho JS, Park MK, Cho SM, Yi H, Cho
• HJ, Shin HC. Development of a sperm preparation method for artificial
• insemination in rabbits. Journal of Animal and Veterinary Advances 2012;11:712–8.
• Ujházy E, Sadlonová I, Dubovick˘ M, Mach M, Múcková M, Flaskárová E. Teratological study of the herbicide 4-chloro-2- methylphenoxyacetic acid in rabbits. J Appl Toxicol 2006;26:368–73.
• Bonanno A, Costanzo D. The effect of physiological and climatic
• factors on the main reproductive traits of artificially inseminated rabbits.
• Rivista di Coniglicoltura 1987;24:33–9.
• Mach K, Trojan V, Nedvedova V. Fertility, growth and feed consumption in California and New Zealand White purebred and crossbred rabbits. Zivocisna Vyriba 1986;44:109–23.
• Bencheikh N. Production de sperme et fertilité du lapin male,
• Oryctolagus cuniculus. Effets de la fréquence de collecte et du type
• génétique. [Internet] Thèse de doctorat, Ecole Nationale Supérieure
• Agronomique de Toulouse, France. Available from: http://prodinra.
• inra.fr/record/110398
• Bonnano A, Budetta G, Alabiso M, Alicata M. Effect of PGSG and
• GnRH treatment on the ovulatory efficiency of rabbits. Acta Medica
• Veterinaria 1990;36:441–51.
• James WH. Evidence that mammalian sex ratios at birth are partially
• controlled by parental hormone levels at the time of conception. J
• Endocrinol 2008;198:3–15.
• Vega MD, Peña AI, Gullón J, Prieto C, Barrio M, Becerra JJ,
• Herradón PG, Quintela LA. Sex ratio in rabbits following modified
• artificial insemination. Anim Reprod Sci 2008;103:385–91.
• Hafez ES. Growth and survival of blastocysts in the domestic rabbit.
• II. Quantitative effects of exogenous progesterone following ovariectomy.J Reprod Fertil 1964;7:241–9.
• Hafez ES. Maternal effects on implantation and related phenomena
• in the rabbit. Experientia 1965;21:234–7.
• Wolterbeek APM, Waalkens-Berendsen DH. Oral embryotoxicity/
• teratogenicity study with trehalose in New Zealand White rabbits.
• TNO Nutrition and Food Research Institute. TNO Peport 1999;98:1–25.
• Jubb KVF, Kennedy PC, Palmer NC. Female genital tract. In: Maxie
• MG, Jubb KVF, Kennedy PC, Palmer N, editors. Pathology of domestic animals. 5th ed. London: Elsevier Saunders; 2007. p. 476.
• Sendag S, Dinc DA, Celik HA, Aydin I, Wehrend A. Sonographische
• Verlaufsuntersuchungen an trächtigen Hündinnen. – ein Beitrag zur
• Bedeutung der Fruchtresorption beim Hund. Tieraerztliche Praxis
• Kleintiere 2010;3:133–8.
• Özalp GR, Temizel EM, Özocak-Batmaz E. Clinical, ultrasonography
• and haematology of aglepristone-inducedmid-gestation pregnancy
• terminations in rabbits. J S Afr Vet Assoc 2013;84:E1–5.