BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ

Yıl 2018, Cilt: 7 Sayı: 2, 227 - 238, 17.08.2018

Seval Cing Yıldırım Turgay Kanat

https://doi.org/10.18036/aubtdc.397326

Öz

Bu
çalışmada oleginous maya olan Rhodotorula
glutinis’ten lipit
üretimi ve ekstre edilen lipitten biyodizel üretimi gerçekleştirilmiştir.
Maksimum lipit üretim koşullarının belirlenmesi amacıyla başlangıç pH’sı,
sıcaklık, çalkalama hızı,  farklı karbon
ve azot kaynakları gibi çeşitli sistem paremetrelerinin etkisi kesikli
fermentasyon sisteminde incelenmiştir. Biyodizel üretim maliyetini azaltmak
için melas ve peynir altı suyu içeren besiyerleri de test edilmiştir. Ayrıca
belirlediğimiz optimum koşullarda iki basamaklı fermentasyon sistemiyle lipit
üretilmiştir. Kültür ortamlarından lipit ekstraksiyonu modifiye Bligh ve Dyer
yöntemine göre gerçekleştirilmiş ve elde edilen lipitler biyodizel üretiminde
kullanılmıştır. Yağ asidi ve yağ asidi metil esterlerinin analizi gaz
kromatoğrafisi-kütle spektrometresi cihazında yapılmıştır. Bu çalışma sonucunda
üretilen maksimum lipit içeriği ve biyodizel verimi sırasıyla  % 29.53±0.46 ve % 80.65 olarak tespit
edilmiştir. Palmitik asit, oleik asit ve stearik asit içeren lipitlerin, yağ
asidi kompozisyonunun biyodizel üretimine uygun olduğu belirlenmiştir. 

Anahtar Kelimeler

Rhodotorula glutinis, lipit, biyodizel, optimizasyon

PRODUTION OF LIPIDS FROM OLEAGIOUS YEAST CELL IN DIFFERENT CULTURAL TYPES AND MEDIUMS FOR USAGE IN BIODIESEL PRODUCTION

Öz

In this study, lipid production from oleaginous yeast Rhodotorula glutinis and biodiesel production from the extracted lipid was performed. In order to determine the maximum lipid production conditions, the effect of various system parameters such as initial pH, temperature, agitation rate, different carbon and nitrogen sources has been examined in batch fermentation system. Media containing molasses and cheese whey were also tested to reduce the cost of biodiesel production. In addition, under optimum conditions, lipid was produced with a two-step fermentation system. Lipid was extracted from culture media according to the modified Bligh and Dyer method and lipids obtained were used for biodiesel production. Analysis of fatty acids and fatty acid methyl esters was carried out on the gas chromatograph-mass spectrometer. As a result of this study, maximum lipid content and biodiesel production were 29.53 ± 0.46% and 80.65%, respectively. It has been determined that fatty acid composition of lipids containing palmitic acid, oleic acid and stearic acid is suitable for biodiesel production.

Anahtar Kelimeler

Rhodotorula glutinis, lipid, biodiesel, optimization

Kaynakça

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