Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle

Yıl 2019, Cilt: 66 Sayı: 3, 311 - 316, 14.06.2019

Neziha Hacıhasanoğlu Çakmak Hasret Yardibi

https://doi.org/10.33988/auvfd.436199

Cited By: 3

Öz

Hereditary diseases cause yield and economic loses. It is important to examine hereditary diseases at the molecular level and to remove diseases from the herd. In our study, it was aimed to determine allele frequencies of genes that cause bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation diseases in Holstein cattle. Blood samples were randomly taken from 300 Holstein cattle in different dairy farms in Kocaeli, Sakarya and Balıkesir provinces. Deoxyribonucleic acid samples were isolated from blood samples by using the standard ammonium acetate salt-out method. The target regions were amplified by polymerase chain reaction to determine the mutant alleles causing bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation. According to the nucleotide chromotograms of the samples subjected to bovine leukocyte adhesion deficiency analysis, it was determined that 4 out of 300 cattle were heterozygous and 296 were homozygous. Polymerase chain reaction procedure for factor XI deficiency disease was sufficient, while samples amplified by polymerase chain reaction for complex vertebral malformation disease were subjected to restriction particle length polymorphism. Factor XI deficiency and complex vertebral malformation disease genes were all homozygous normal.

Anahtar Kelimeler

Anaemia, β2 integrin glycoprotein, Holstein, point mutation, PCR-RFLP, SLC35A3

Kaynakça

• Ackermann MR, Kehrli ME, Morfitt DC (1993): Ventral dermatitis and vasculitis in a calf with bovine leukocyte adhesion deficiency. J Am Vet Med Assoc, 202, 413-415.
• Agerholm S, Bendixen C, Andersen O, et al. (2001): Complex vertebral malformation in Holstein calves. J Vet Diagn Invest, 13, 283-289.
• Agerholm JS, Andersen O, Almskou MB, et al. (2004): Evaluation of the inheritance of the complex vertebral malformation syndrome by breeding studies. Acta Vet Scand, 45, 133-137.
• Akyüz B (2004) Türkiye’deki Holştayn sığırlarında sığır lökosit bağlanma yetmezliğinin (bovineleukocyte adhesion deficiency, BLAD) restriksiyon parçacık uzunluk polimorfizmi (restriction fragment length polymorphism, RFLP) ile belirlenmesi. Doktora Tezi. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Zootekni Programı. Ankara.
• Akyüz B (2013): Türkiye’deki iki farklı işletmede yetiştirilen Holştayn boğalarda faktör XI yetmezliği (FXID) allel frekansının belirlenmesi. Kafkas Üniv Vet Fak Derg, 19(1), 127-131.
• Bagheri S, Amirinia C, Chamani M, et al. (2012): Identification of factor XI deficiency in Khuzestan Buffalo population of Iran. Global Vet, 8(6), 598-600.
• Batt CA, Wagner P, Wiedmann M, et al. (1994): Detection of bovine leukocyte adhesion deficiency by nonisotopic ligase chain peaction. Anim Genet, 25, 95-98.
• Chu Q, Sun D, Yu Y, et al. (2008): Identification of complex vertebral malformation carriers in Chinese Holstein. J Vet Diagn Invest, 20, 228-230.
• Citek J, Rehout V, Hajkova J, et al. (2006): Monitoring of the genetic health of cattle in the Czech Republic. Vet Med Czech Rep, 51(6), 333-339. Eydivandi C, Amirinia C, Jamah-Kasha NE, et al. (2011): Study of factor XI deficiency in Khuzestan cattle population of Iran. African J Biotechnol, 10(4), 718-721.
• Ghanem ME, Nishibori M, Nakao T, et al. (2005): Factor XI mutation in a Holstein cow with repeat breeding in Japan. J Vet Med Sci, 67(7), 713-715. Ghanem ME, Nakao T, Nishibori M (2006): Deficiency of uridine monophosphate syntheses (DUMPS) and X-chromosome deletion in fetal mummification in cattle. Anim Rep Sci, 91, 45-54.
• Ghanem ME, Akita M, Suzuki T, et al. (2008): Complex vertebral malformation in Holstein cows in Japan and its inheritance to crossbred F1 generation. Anim Rep Sci, 103, 348-354.
• Hemati B, Gharaie-Fathabad S, Fazeli MH, et al. (2015): Investigation of bovine leukocyte adhesion deficiency (BLAD) and complex vertebral malformation (CVM) in a population of Iranian Holstein cows. Iranian J Appl Anim Sci, 5(1), 69-72.
• Karsli T, Şahin E, Argun Karsli B, et al. (2011): Identification of alleles for faktör XI (FXID) and uridine monophosphate sythase (DUMPS) deficiencies in Holstein cows reared in Antalya. Kafkas Üniv Vet Fak Derg, 17(3), 503-505.
• Korkmaz Agaoğlu Ö, Agaoğlu AR, Saatcı M (2015): Estimating allele frequencies of some hereditary diseases in Holstein cattle reared in Burdur province, Turkey. Turkish J Vet Anim Sci, 39, 338-342.
• Kulakli GN, Akyüz B (2011): Kayseri bölgesinde yetiştirilen Holştayn sığırlarında kompleks vertebral malformasyon hastalığı geninin allel frekanslarının belirlenmesi. Erciyes Üniv Vet Fak Derg, 8(2), 69-74.
• Marron BM, Robinson JL, Gentry PA, et al. (2004): Identification of a mutation associated with factor XI deficiency in Holstein cattle. Anim Genet, 35(6), 454-456.
• Meydan H, Yildiz MA, Ozdil F, et al. (2009): Identification of factor XI deficiency in Holstein cattle in Turkey. Acta Vet Scand 51, 5,. Meydan H, Yildiz MA, Agerholm JS (2010): Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey. Acta Vet Scand, 52, 56.
• Miller M, Dykes DD, Polesky HF (1985): A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res, 16, 1215-1219.
• Mondal K, Chakravarti S, Ghosh AK, et al. (2016): Novel identification of factor XI deficiency in Indian Sahiwal (Bos indicus) cattle. Mol Biol Rep 43, 213-219.
• Mukhopadhyaya PN, Jha M, Muraleedharan P, et al. (2006): Simulation of the normal, carrier and affected controls for large-scale genotyping of cattle for factor XI deficiency. Genet Mol Res 5(2), 323-332.
• Patel M, Patel RK, Singh KM, et al. (2011): Detection of genetic polymorphism in CD18 gene in cattle by PCR-RFLP. Woyamba J Anim Sci, 578, 110-111.
• Roy A, Kotikalapudu R, Patel RK, et al. (2012): New cases of bovine leukocyte adhesion deficiency (BLAD) carriers in Indian Holstein cattle. Int J Vet Sci, 1(2), 80-82.
• Saberi J, Hemati B, Noshary A (2014): Studying the genetic defects of CVM in Holstein cows of Alborz province. Bullet Env Pharma Life Sci, 3(12), 38-41.
• Şahin E, Karslı T, Galiç A, et al. (2013): Identification of bovine leukocyte adhesion deficiency (BLAD) and bovine citrullinaemia (BC) alleles in Holstein cows reared in Antalya region. J Appl Anim Res, 41(1), 56-60.
• Thomsen B, Horn P, Panitz F, et al. (2006): A missence mutation in the bovine SLC35A3 gene, encoding a UDP-N-acetylglucosamine transporter causes complex vertebral malformation. Genom Res, 16, 97-105.
• Wang C, Tong Q, Hu XZ, et al. (2011): Identification of complex vertebral malformation carriers in Holstein cattle in South China. Genet Mol Res, 10(4), 2443-2448.
• Wang S, Hao H, Zhao X, et al. (2012): A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle. J Vet Diagn Invest, 24(3), 568-571.
• Yaşar G, Akyüz B (2012): Kayseri civarında yetiştirilen Holştayn ineklerde kalıtsal Faktör XI yetmezliği geninin allel frekanslarının belirlenmesi. Erciyes Üniv Vet Fak Derg, 9(1), 7-12.
• Yeh F, Yang RC, Boyle T (2000): Popgene (v.1.32) Microsoft Windows-based freeware for Population Genetic Analysis. Retrieved from http://www.ualberta.ca/~fyeh/popgene.
• Zhang Y, Fan X, Sun D, et al. (2012): A novel method for rapid and reliable detection of complex vertebral malformation and bovine leukocyte adhesion deficiency in Holstein cattle. J Anim Sci Biotechnol, 3, 24.