Mutation induction using Co60 in Pembe cekirdeksiz (Vitis vinifera L.) grape cultivar

Yıl 2017, Cilt: 6 Sayı: 2, 101 - 106, 30.12.2017

Hatice Bilir Ekbiç Semih Tangolar Ercan Ekbiç

https://doi.org/10.29278/azd.371737

Cited By: 1

Öz

In the study an optimum dose among Co⁶⁰ treatments that make less physical injury and positive effects comparing the control plants was investigated in Pembe Cekirdeksiz grape cultivar. Related to this aim, vine cuttings were exposed to the 0, 15, 25, 35 and 45 Gy doses of Co⁶⁰. Buds of radiation treated cuttings were grafted on 17 old vines of Recel grape cultivars (Vitis vinifera L.) by chip bud grafting method. Survival rates obtained from 15 and 25 Gy Co⁶⁰ applications were found to be the closest to the control application. 35 and 45 Gy doses of Co⁶⁰ were observed as lethal in this cultivar. It was observed that progressive radiation doses resulted in a variation in shoot length and leaf area values. The highest shoot length and leaf area values were obtained from control and 15 Gy Co⁶⁰ applications. Radiation treatment made bit late in the bud break but it did not retard the ripening time. On the other hand Co⁶⁰ treatment resulted decreasing in cluster and berry size. Stomata numbers decreased with the progressive Co⁶⁰ doses. Although same bit variations in different characteristics were determined by radiation treatments, positive variations for grape breeding were not observed at this stage.

Anahtar Kelimeler

Grapevine, mutation, gamma irradiation

Kaynakça

• Ahloowalia, B. S., Maluszynski, M., Nicterlein, K., 2004. Global Impact of Mutation-Derived Varieties. Euphytica, 135: 187-204.
• Akal, N., 2001. Değişik Vitis Türleri ve Melezlerinin Vejetatif Organları Arasındaki Bazı Anatomik Farklılıkların Belirlenmesi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, Adana, 71 s.
• Blanco, C. A. 2005. Mutagenesis en la Mejora Genetica Vegetal. Curso Notas de Especializacion Postuniversitaria del Programa Master en Mejora Genetica Vegetal, 204 p.
• Coban, H., Kara, S., Ilter, E., 2002. Investigations on Raidiosensitivity of Some Grape Varieties. Pakistan Journal of Biological Sciences, 5(5): 601-603.
• Dardeniz A., Tayyar, S., 2005. An Investigation on The Bud Break and Growth of Cuttings of 420 A and 5 BB American Vine Rootstocks Irradiated with Different Gamma Doses. Journal Central European Agriculture, 6(2): 173-178.
• Donini, S., 1998. Induced Mutation in Plant Breeding: Current Status and Future Outlook. Kluwer Academic Publishers, Dordrecht, 225-291 s.
• Einset, J., Pratt, C., 1975. Grapes (In: Advances in Fruit Breeding. Ed.: J. Janick and J.N. Moore). Purdue University Press. West Lafayette, Indiana, 130-153 s.
• Kaya, A., 2002. İyonize Radyasyonun Biyolojik Etkileri. Dicle Tıp Dergisi, 29 (3): 65-75.
• Maluszynski, M., Nicterlein, K., Van Zanten, L., Ahloowalia, B. S., 2000. Officially Released Mutant Varieties. The FAO/IAEA Database. Mut. Breed. Rev., 12: 1-84.
• Marasalı, B., Kunter, B., Değirmenci, D., Keskin, N., Taner, Y., Çelik, H., Tutluer, İ., Uslu, N., Sağel, Z., 2003. Yerli Üzüm Çeşitlerinde Mutasyon Islahına Yönelik Olarak Etkili Mutasyon Dozunun Belirlenmesi ve M1V1 Generasyonunda Görülen İlk Etkiler. Türkiye IV. Ulusal Bahçe Bitkileri Kongresi. 467-470 s.
• Meksem, K., Kahl, G., 2010. The Handbook of Plant Mutatition Screening. WILEY-VCH Verlag GmbH and Co KGaA., Weinheim, 435 p.
• Pannuswami, V., Iralappon, I., Anomugam, R., 1991. Sensitivity of Muscat Grape Cuttings to Gamma Irradiation. South Indian Horticulture, 39(5): 317-318.
• Predieri, S., 2001. Mutation Induction and Tissue Culture in Improving Fruits. Plant Cell, Tissue and Organ Culture, 64: 185-210.