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Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Yıl 2018, Cilt: 7 Sayı: 2, 179 - 184, 26.12.2018
https://doi.org/10.29278/azd.476349

Öz

Light is not only
effective in photosynthesis, growth and development, but also play an important
role in biosynthesis of primary and secondary metabolites. The present research
was aimed to determine effect of light on biosynthesis of alkamide, caffeic
acid derivatives and echinacoside in cell suspension cultures of
Echinacea purpurea L. Stem explants
derived from sterile plants were subjected to callus culture in a B5 media
supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA. Afterward, 8-day cell cultures
were incubated in light and dark environments and cell harvesting, with a
three-day interval, was carried out five times. The contents of alkamide,
caffeic acid derivatives and echinacoside were determined using HPLC. Alkamide,
caftaric acid and echinacoside content increased regularly with light
application. Light application increased alkamide content by 57%, as an
average, compared to darkness. The cell cultures exposed to 12 days of
incubation in light produced the highest alkamide content, with a 2.11-fold
increase, compared to dark. Subjecting the cell cultures to light for 12 days
resulted in 70% and 63% increases in caftaric acid and echinacoside. This study
showed that light application in
Echinacea
purpurea
L. cell suspension culture had a great potential for increasing some
important phytochemicals.

Kaynakça

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Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Muhammed Akif Açıkgöz Bu kişi benim

Şevket Metin Kara

Ebru Batı Ay Bu kişi benim

Sedanur Odabaş Bu kişi benim

Yayımlanma Tarihi 26 Aralık 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 7 Sayı: 2

Kaynak Göster

APA Açıkgöz, M. A., Kara, Ş. M., Batı Ay, E., Odabaş, S. (2018). Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures. Akademik Ziraat Dergisi, 7(2), 179-184. https://doi.org/10.29278/azd.476349