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DÜŞÜK DOZ X IŞINLARININ SIÇAN MİDESİNDE OLUŞTURDUĞU HASARA KARŞI PRUNUS ARMENIACA L. (KAYISI)’NIN KORUYUCU VE TEDAVİ EDİCİ ETKİSİ

Yıl 2014, Cilt: 3 Sayı: 2, 108 - 113, 31.08.2014

Öz

AMAÇ: Düşük doz radyasyon gastrointestinal sistem üzerinde zararlı etkiler göstermektedir.


YÖNTEMLER: Bu çalışmada, 60 adet Sprague-Dawley sıçan 6 gruba ayrıldı. Grup 1: 28 hafta normal diyet alan grup. Grup 2: 20 hafta normal diyet alıp, son 8 hafta normal diyet+radyasyon alan grup. Grup 3: 28 hafta kayısılı diyet alan grup. Grup 4: 20 hafta kayısılı diyet alıp, son 8 hafta kayısılı diyet+radyasyon alan grup. Grup 5: 8 hafta normal diyet alıp, devam eden 20 hafta kayısılı diyet alan grup. Grup 6: 8 hafta normal diyet alıp, devam eden 20 hafta kayısılı diyet+radyasyon alan grup. Deney sonunda mide dokuları çıkarıldı. Doku örnekleri rutin histolojik prosedürden geçirildi. Kesitlere hematoksilen-eozin (H-E) boyası yapıldı. Histopatolojik hasar (mukoza hasar, konjesyon, infiltrasyon, gastrik bezlerin dilatasyonu, hemoraji) skoru hesaplandı. Maksimum skor 15 idi. İstatistiksel analizler için SPSS 13.0 ve MedCalc 11.0 programları kullanıldı. Tüm veriler aritmetik ortalama±Standart hata olarak ifade edildi.

BULGULAR: Grup 1, 3 ve 5’e ait kesitler normal histolojik görünümdeydi. Grup 2’de radyasyona bağlı yaygın mide hasarı tespit edildi. Ortalama histopatolojik hasar skoru 8.20±0.32 idi. Grup 4 ve 6’ya ait hasar skoru sırasıyla 2.30±0.47 ve 2.20±0.66 idi. Grup 4 ve 6’ya ait hasar skoru, grup 2’den anlamlı derecede düşüktü (P=0.0001).


SONUÇ: Sonuç olarak, radyasyon ile oluşan mide hasarında kayısının faydalı olduğunu düşünmekteyiz.

Kaynakça

  • 1. Fenech M, Chang WP, Krisch VM, Holland N, Bonnassi S, Zeiger E. HUMN Project: Detailed description of the scoring criteria for the cytokinesis block micronucleus assay using isolated human lymphocyte cultures. Mutat Res. 2003;534:65-75.
  • 2. Yaren H, Karayılanoğlu T. Raditation and effects on human health. TSK Koruyucu Hekimlik Bülteni. 2005;4(4):199-208.
  • 3. Ugras M, Kurus M, Ates B, Soylemez H, Otlu A, Yılmaz I. Prunus armeniaca L. (apricot) protects rat testes from detrimental effects of low-dose x-rays. Nutrition Research. 2010;30:200-8.
  • 4. Apte MV, Wilson JS, Korsten MA. Alcohol related pancreatic damage: Mechanisms and Treatment. 1997;21:13-20.
  • 5. Husain K, Scott BR, Reddy SK, Somani SM. Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system. Alcohol. 2001;25:89-97.
  • 6. Aase S. Disturbances in the balance between aggressive and protective factors in the gastric and duodenal mucosa. Scand J Gastroenteral. 1989;24:17-23.
  • 7. Allen A, Leonarn JA. The mucos barrier: Its role in gastroduodenal mucus protection. J Clin Gastroenterol. 1980;1:593-98.
  • 8. Reinhart WH, Muller O, Halter F. Influence of long term 16, 16 dimethyl prostaglandin E2 treatment on the rat gastrointestinal mucosa. Gastroenterology. 1983;85:1003-10.
  • 9. Hamilton E, Franks LM. Cell proliferation and aging in mouse colon late effects of repeated xirradiation in young and old mice. Europ J Cancer. 1980;16:663-69.
  • 10. Black WC, Gomez LS, Yuhas JM, Kligermen MM. Quantitation of the late effects of X radiation on the large intestine. Cancer. 1980;45(3):444-51.
  • 11. Parks DA, Bulkley GB, Granger DN. Role of oxygen derived free radicals indigestive tract diseases. Surgery. 1983;94:415-22.
  • 12. Kurus M, Ugras M, Ates B, Otlu A. Apricot ameliorates alcohol induced testicular damage in rat model. Food and Chemical Toxicology. 2009;47:2666-72.
  • 13. Ozturk F, Gul M, Ates B, Ozturk IC, Cetin A, Vardi N, Otlu A, Yılmaz I. Protective effect of apricot (Prunus armeniaca L.) on hepatic steatosis damage induced by carbon tetrachloride in Wistar rats. British Journal of Nutrition. 2009;102:1767-75.
  • 14. Güçlü K, Altun M, Özyürek M, Karademir SE, Apak R. Antioxidant capacity of fresh, sun- and sulphited-dried Malatya apricot (Prunus armeniaca) assayed by CUPRAC, ABTS/TEAC and folin methods. International Journal of Food Science and Technology. 2006;41(1):76-85.
  • 15. Munzuroglu O, Karatas F, Geckil H. The vitamin and selenium contents of apricot fruit of different varieties cultivated in different geographical regions. Food Chemistry. 2003;83:205-12.
  • 16. Parlakpınar H, Olmez E, Acet A, Ozturk F, Tasdemir S, Ates B, et al. Beneficial effects of apricot-feeding on myocardial ischemia-reperfusion injury in rats. Food Chem Toxicol. 2009;47:802-8.
  • 17. Kaya A. İyonizan radyasyonun biyolojik etkileri. Dicle Tıp dergisi. 2002;29:3.
  • 18. Özalpan A. Temel Radyobiyoloji. 1. Basım. İstanbul: Haliç Üniversitesi Yayınları. 2001:1-218.
  • 19. Withers RH. Biologic basis of radiation therapy. Principles and practice of radiation oncology. Perez C, Brady L, ed. LippincottCo. Philadelphia.1992;64-98.
  • 20. Steel GG. The Significance of Radiobiology for Radiotheraphy. In; Steel GG (Ed.). Basic Clinical Radiobiology. New York: Co-published in the USA by Oxford University Pres. 1997:1-7.
  • 21. Raguso CA, Leverve X, Pichard C. Protective effects of recombinant growth hormone on intestinal mucosa in rats receiving abdominal radiotheraphy. ClinicalNutrition. 2002;21:487-90.
  • 22. Abdel-Salam OM, Hadajat I, Bayomy AR, El-Shinawy S, Arbid MS. Acute effect of gamma irradiation on gastric acid secretion and gastric mucosal integrity in the rat. Scientific World Journal. 2005;5:195-204.
  • 23. Dubois A, Walker RI. Prospects for managementof gastrointestinal injury associated with the acute radiation syndrome. Gastroenterology. 1988;95(2):500-7.
  • 24. Breiter N, Trott KR, Sassy T. Effect of X-irradiation on the stomach of the rat. Int J Radiat Oncol Biol Phys. 1989;17(4):779-84.
  • 25. Dubois A, Danquechin DE, Wood LR, Rogers JE, O'Connell L, Durakovic A, et al. Effect of gamma-irradiation on the healing of gastric biopsy sites in monkeys. An experimental model for peptic ulcer disease and gastric protection.Gastroenterology. 1985;88:375-81.
  • 26. Tsay F-W, Lo G-H, Lai K-H, Lin C-K, Chen H-H, Hsu P-I. Successful Treatment of Radiation-Induced Hemorrhagic Gastritis by Prednisolone. J Int Med Taiwan. 2006;17(1):39-42.
  • 27. Marshall GT, Thirlby RC, Bredfeldt JE, Hampson NB. Treatment of gastrointestinal radiation injury with hyperbaric oxygen. Undersea Hyperb Med. 2007;34(1):35-42.
  • 28. Yanagihara K, Nii M, Numoto M, Kamiya K, Tauchi H, Sawada S, et al. Radiation-induced apoptotic cell death in human gastric epithelial tumour cells; correlation between mitotic death and apoptosis. Int J Radiat Biol. 1995;67(6):677-85.
  • 29. Dubois A, Dorval ED, Steel L, Fiala NP, Conklin JJ. Effect of ionizing radiation on prostaglandins and gastric secretion in rhesus monkeys. Radiat Res. 1987;110(2);289-93.

PROTECTIVE AND THERAPEUTIC EFFECTS OF PRUNUS ARMENIACA L. (APRICOT) AGAINST LOW DOSE RADIATION-INDUCED GASTRIC DAMAGE

Yıl 2014, Cilt: 3 Sayı: 2, 108 - 113, 31.08.2014

Öz

OBJECTIVE: Low-dose of radiation is harmful effects to the gastrointestinal system.


METHODS: 60 male Sprague-Dawley rats divided into 6 groups. Group 1: Rats on a regular diet for 28 weeks. Group 2: Rats on a regular diet for 28 weeks, X-Ray on last day of eighth week. Group 3: Rats on an apricot diet for 28 weeks, Group 4: Rats on an apricot diet for 28 weeks, X-Ray on last day of eighth week. Group 5: Rats on a regular diet for 8 weeks, followed by an apricot diet for the following 20 weeks, Group 6: Rats on a regular diet for 8 weeks, X-Ray on last day of eighth week, followed by an apricot diet for 20 weeks. At the end of the experimentation stomach tissuses were removed. The tissue samples were processed routine histological procedures. Sections stained with H-E. Histopathologic damage score was calculated in regard to damage of mucosa, vascular congestion, infiltration, dilatation of the gastric glands and hemorrhage. Maximum score was 15. Statistical analysis was carried out using the SPSS 13.0 and MedCalc 11.0 statistical programs. All data are expressed as arithmetic mean±SE.

RESULTS: The sections from group 1, 3 and 5 were normal in histological appearance. In group 2, severe radiation-induced gastric damage was detected. The MHDS was 8.20±0.32 in this group. Findings in Group 4 and 6, were significantly lower than Group 2 (P=0.0001). The MHDS of Group 4 and 6 was 2.30±0.47 and 2.20±0.66, respectively.


CONCLUSION: We concluded that apricot administration reduced radiation-induced stomach injury.

Kaynakça

  • 1. Fenech M, Chang WP, Krisch VM, Holland N, Bonnassi S, Zeiger E. HUMN Project: Detailed description of the scoring criteria for the cytokinesis block micronucleus assay using isolated human lymphocyte cultures. Mutat Res. 2003;534:65-75.
  • 2. Yaren H, Karayılanoğlu T. Raditation and effects on human health. TSK Koruyucu Hekimlik Bülteni. 2005;4(4):199-208.
  • 3. Ugras M, Kurus M, Ates B, Soylemez H, Otlu A, Yılmaz I. Prunus armeniaca L. (apricot) protects rat testes from detrimental effects of low-dose x-rays. Nutrition Research. 2010;30:200-8.
  • 4. Apte MV, Wilson JS, Korsten MA. Alcohol related pancreatic damage: Mechanisms and Treatment. 1997;21:13-20.
  • 5. Husain K, Scott BR, Reddy SK, Somani SM. Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system. Alcohol. 2001;25:89-97.
  • 6. Aase S. Disturbances in the balance between aggressive and protective factors in the gastric and duodenal mucosa. Scand J Gastroenteral. 1989;24:17-23.
  • 7. Allen A, Leonarn JA. The mucos barrier: Its role in gastroduodenal mucus protection. J Clin Gastroenterol. 1980;1:593-98.
  • 8. Reinhart WH, Muller O, Halter F. Influence of long term 16, 16 dimethyl prostaglandin E2 treatment on the rat gastrointestinal mucosa. Gastroenterology. 1983;85:1003-10.
  • 9. Hamilton E, Franks LM. Cell proliferation and aging in mouse colon late effects of repeated xirradiation in young and old mice. Europ J Cancer. 1980;16:663-69.
  • 10. Black WC, Gomez LS, Yuhas JM, Kligermen MM. Quantitation of the late effects of X radiation on the large intestine. Cancer. 1980;45(3):444-51.
  • 11. Parks DA, Bulkley GB, Granger DN. Role of oxygen derived free radicals indigestive tract diseases. Surgery. 1983;94:415-22.
  • 12. Kurus M, Ugras M, Ates B, Otlu A. Apricot ameliorates alcohol induced testicular damage in rat model. Food and Chemical Toxicology. 2009;47:2666-72.
  • 13. Ozturk F, Gul M, Ates B, Ozturk IC, Cetin A, Vardi N, Otlu A, Yılmaz I. Protective effect of apricot (Prunus armeniaca L.) on hepatic steatosis damage induced by carbon tetrachloride in Wistar rats. British Journal of Nutrition. 2009;102:1767-75.
  • 14. Güçlü K, Altun M, Özyürek M, Karademir SE, Apak R. Antioxidant capacity of fresh, sun- and sulphited-dried Malatya apricot (Prunus armeniaca) assayed by CUPRAC, ABTS/TEAC and folin methods. International Journal of Food Science and Technology. 2006;41(1):76-85.
  • 15. Munzuroglu O, Karatas F, Geckil H. The vitamin and selenium contents of apricot fruit of different varieties cultivated in different geographical regions. Food Chemistry. 2003;83:205-12.
  • 16. Parlakpınar H, Olmez E, Acet A, Ozturk F, Tasdemir S, Ates B, et al. Beneficial effects of apricot-feeding on myocardial ischemia-reperfusion injury in rats. Food Chem Toxicol. 2009;47:802-8.
  • 17. Kaya A. İyonizan radyasyonun biyolojik etkileri. Dicle Tıp dergisi. 2002;29:3.
  • 18. Özalpan A. Temel Radyobiyoloji. 1. Basım. İstanbul: Haliç Üniversitesi Yayınları. 2001:1-218.
  • 19. Withers RH. Biologic basis of radiation therapy. Principles and practice of radiation oncology. Perez C, Brady L, ed. LippincottCo. Philadelphia.1992;64-98.
  • 20. Steel GG. The Significance of Radiobiology for Radiotheraphy. In; Steel GG (Ed.). Basic Clinical Radiobiology. New York: Co-published in the USA by Oxford University Pres. 1997:1-7.
  • 21. Raguso CA, Leverve X, Pichard C. Protective effects of recombinant growth hormone on intestinal mucosa in rats receiving abdominal radiotheraphy. ClinicalNutrition. 2002;21:487-90.
  • 22. Abdel-Salam OM, Hadajat I, Bayomy AR, El-Shinawy S, Arbid MS. Acute effect of gamma irradiation on gastric acid secretion and gastric mucosal integrity in the rat. Scientific World Journal. 2005;5:195-204.
  • 23. Dubois A, Walker RI. Prospects for managementof gastrointestinal injury associated with the acute radiation syndrome. Gastroenterology. 1988;95(2):500-7.
  • 24. Breiter N, Trott KR, Sassy T. Effect of X-irradiation on the stomach of the rat. Int J Radiat Oncol Biol Phys. 1989;17(4):779-84.
  • 25. Dubois A, Danquechin DE, Wood LR, Rogers JE, O'Connell L, Durakovic A, et al. Effect of gamma-irradiation on the healing of gastric biopsy sites in monkeys. An experimental model for peptic ulcer disease and gastric protection.Gastroenterology. 1985;88:375-81.
  • 26. Tsay F-W, Lo G-H, Lai K-H, Lin C-K, Chen H-H, Hsu P-I. Successful Treatment of Radiation-Induced Hemorrhagic Gastritis by Prednisolone. J Int Med Taiwan. 2006;17(1):39-42.
  • 27. Marshall GT, Thirlby RC, Bredfeldt JE, Hampson NB. Treatment of gastrointestinal radiation injury with hyperbaric oxygen. Undersea Hyperb Med. 2007;34(1):35-42.
  • 28. Yanagihara K, Nii M, Numoto M, Kamiya K, Tauchi H, Sawada S, et al. Radiation-induced apoptotic cell death in human gastric epithelial tumour cells; correlation between mitotic death and apoptosis. Int J Radiat Biol. 1995;67(6):677-85.
  • 29. Dubois A, Dorval ED, Steel L, Fiala NP, Conklin JJ. Effect of ionizing radiation on prostaglandins and gastric secretion in rhesus monkeys. Radiat Res. 1987;110(2);289-93.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Meltem Kuruş Bu kişi benim

Hülya Elbe Bu kişi benim

Elif Taşlıdere Bu kişi benim

Murat Uğraş Bu kişi benim

Ali Otlu Bu kişi benim

Yayımlanma Tarihi 31 Ağustos 2014
Gönderilme Tarihi 20 Aralık 2013
Yayımlandığı Sayı Yıl 2014 Cilt: 3 Sayı: 2

Kaynak Göster

APA Kuruş, M., Elbe, H., Taşlıdere, E., Uğraş, M., vd. (2014). DÜŞÜK DOZ X IŞINLARININ SIÇAN MİDESİNDE OLUŞTURDUĞU HASARA KARŞI PRUNUS ARMENIACA L. (KAYISI)’NIN KORUYUCU VE TEDAVİ EDİCİ ETKİSİ. Balıkesir Sağlık Bilimleri Dergisi, 3(2), 108-113.

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