KISA SÜRELİ TEKE SPERMASINININ SAKLANMASINDA

Yıl 2017, Cilt: 6 Sayı: 3, 104 - 107, 31.12.2017

Çiğdem Çebi Şen Recai Kulaksız

Öz

GİRİŞ ve AMAÇ: Bu çalışmanın amacı, Ankara tekesi spermasının kısa süreli saklanmasında sperma sulandırıcılarına katılan farklı miktarlardaki farklı şekerlerin (glukoz ve fruktoz) ve yumurta sarısının teke sperması kalitesi üzerine etkinliğinin belirlenmesidir.

YÖNTEM ve GEREÇLER: : 4 Ankara tekesinden suni vajen yardımıyla alınan ejakulatlar 8 eşit kısma ayrılarak farklı dozlarda glukoz ve fruktoz içeren ve yumurta sarısı içermeyen yağsız süt tozu sulandırıcısı ile sulandırıldı. Sulandırılan sperma örnekleri 0.25 ml’lik payetlere çekilerek +4°C’de 0, 24, 48, 72 ve 96 saatlerde spermatozoa motilitesi yönünden muayene edildi.

BULGULAR: Yumurta sarısı içermeyen grubun kısa süreli saklama sonucu motilite değerlerindeki düşüşün özellikle ilk 24 saat içerisinde hızla gerçekleştiği gözlendi. Farklı dozda glukoz veya fruktozun sulandırıcıya eklenmesi ise spermatozoa motilitesi bakımından 0-96. saatler arasında istatistiksel olarak önemli bir farklılık oluşturmadı..

TARTIŞMA ve SONUÇ: Sonuç olarak teke spermasının kısa sureli saklanması için süt bazlı sulandırıcılara şeker olarak eklenen glukoz veya fruktozun kullanımı arasında bir fark yoktur.

Anahtar Kelimeler

Glukoz, fruktoz, teke sperması, yumurta sarısı

INFLUENCE OF SPERM QUALITY OF DIFFERENT SUGARS DURING SHORT-TERM STORAGE OF GOAT SEMEN

Öz

INTRODUCTION: The purpose of the present study is to determine the effect of different sugars (glucose and fructose) and egg yolks effect on the quality of the Ankara goat semen.

METHODS: Ejaculates collected from artificial vagina from 4 Ankara goats were divided into 8 equal parts. Sperma samples containing glucose and fructose at different doses and containing no egg yolk were diluted with skimmed milk.Semen samples were withdrawn with 0.25 ml straws and spermatozoa motility was examined at 0, 24, 48, 72 and 96 hours at + 4 ° C.

RESULTS: The decrease in the short term storage motility values of the group without egg yolks observed especially during the first 24 hours. The addition of different doses of glucose and fructose to the diluent, spermatozoa motility did not make a statistically significant difference between the 0-96 hours.

DISCUSSION AND CONCLUSION: As a result, there is no difference between the use of glucose or fructose added as a milk-based diluent sugar for short-term storage of goat semen.

Anahtar Kelimeler

Glucose, fructose, goat semen

Kaynakça

• 1. Ax RL, Dally MA, Lenz RW, Love CC, Varner DD, Hafez B, Bellin ME. Semen Evaluation. In: Reproduction in Farm Animals, Hafez, B. and E.S.E. Hafez (Eds.). 7th Edn. Lippincott Williams and Wilkins, Philadelphia, ISBN-13: ISBN-13: 978-0683305777, pp: 365-375. 2000.
• 2. Aboagla EM, Terada T. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod. 2003;69,1245-1250.
• 3. Iritani A, Nishika WA. Studies on the egg yolk coagulating factors in goat semen: II properties of the coagulating factor and influential conditions for foagulation. In: Proceedings of Silver Jubilee Laboratory of Animal Husbandry, Kyoto University, 97-104, 1961.
• 4. Sawyer DE, Brown DB. The use on an in vitro sperm activation assay to detect chemically induced damage of human sperm nuclei. Reprod Toxicol, 1995;9, 351-357.
• 5. R Kulaksız, A Daşkın. Teke spermasının kısa ve uzun süreli saklanması. Veteriner Hekimler Derneği Dergisi, 2007;78(4),51-56.
• 6. Pellicer-Rubio MT, Magallon T, Yves. Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycoprotein with triglyceride lipase activity. Biology of Reproduction, 1997;57,1023-1031.
• 7. Ahmad E, Aksoy M. Trehalose as a cryoprotecive agent for the sperm cells: A mini review. Animal Health, Prod. and Hyg, 2012;1(2),123-129.
• 8. Ferdinand N, Thomas TT, Augustave K, Henry DF, Fernand T, Etienne PT. Effects of Buck Age, Storage Duration, Storage Temperature and Diluent on Fresh West African Dwarf Buck Semen. J Reprod Infertility, 2012;3(3),58-66.
• 9. Mann T, Lutwak-Mann C. Examination of spermatozoa and isolated structural components. In: Mann, T. and Lutwak-Mann, C. (Eds) Male reproductive function and semen. Berlin Heidelberg, New York, pp . 63-69, 1981.
• 10. Corteel JM. Viabilite’ des spermatozoid de bouc conserves et congele's avec ou sans leur plasma seminal: effect du glucose (viability of spermatozoa deep frozen with or without seminal plasma: glucose effect). Ann Biol Anim Biochem Biophys, 1974;14, 741-745.
• 11. Fukuhara R, Nishikawa Y. Effects of pH, sperm concentration, washing and substrate concentration on respiration and motility of goat spermatozoa. Jpn J Zootech Sci, 1973;44,266-270.
• 12. Purdy PH. A review on goat sperm cryopreservation. Small Ruminant Res, 2006;63(3),215-225.
• 13. Ponglowhapan S, Essén-Gustavsson B, Forsberg CL. Influence of glucose and fructose in the extender during long-term storage of chilled canine semen. Theriogenology, 2004;62(8),1498-1517.
• 14. Naing SW, Wahid H, Mohd Azam K, Rosnina Y, Zuki AB, Kazhal S, Bukar MM, Thein M, Kyaw T, San MM. Effect of sugars on characteristics of Boer goat semen after cryopreservation. Anim Reprod Sci, 2010;122(1-2),23-28.