Oxidative Damage of Chronic Water Avoidance and the Effect of Fulvic Acid in Male Rat Tissues with Erectile Dysfunction

Yıl 2018, Cilt: 5 Sayı: 3, 112 - 119, 28.09.2018

İbrahim Söğüt Ezgi Yaprak Söyük Esra Çikler-dülger Canan Hürdağ

Öz

Objectives : We aimed to utilize the antioxidant property
of fulvic acid in our study to prevent penile tissue damage associated with
erectile dysfunction.

Material and
Methods: 18 Sprague-Dawley
male adult rats were distributed into 3 experimental groups: Control (C),
Chronic Stress (CS) and Water Avoidance Stress+Fulvic Acid (CS+FA). The
histological sections obtained from the testes has been stained with the
Hematoxylin&Eosin (H&E) and Masson Trichrome and endothelial and n
nitric oxide sentase (eNOS &nNOS) immünohistochemistry labelling has been
made to. Total antioksidant status (TAS), total oksidant status (TOS),
oxidative stress (OSI), catalase (CAT), glutation peroksidase (GPx) and
superoxide dismutase (SOD) levels has been evaluated biochemically.

Results: In CS group were examined, it was observed
that trabecular endothelial cells are lost, collagen fibers had shrunk,
connective tissue and smooth muscle distributions were unevenly distributed
compared to the control group. When CS + FA group were examined, damage in the
CS group showed considerable recovery in tissues that were treated. In CS group
both eNOS and nNOS reactivity had decreased compare to the control group
whereas caveolin-1 reactivity had increased. histochemical data. It was also observed increased TOS and OSI levels and
decreased TAS, SOD, CAT and GPx levels in the KS group.

Conclusion: The fulvic acid used was found to have therapeutic effects but
the dosage used for treatment was not sufficient. We suggest to work at
different but higher dosages to ensure that the therapeutic effect is achieved.

Anahtar Kelimeler

Erectile dysfunction, water avoidance stress, oxidative stress, infertility, fulvic acid

Kronik Sudan Kaçınma Stresi ile Oluşturulan Oksidatif Hasar ve Fulvik Asidin Erkek Sıçan Dokusunda Erektil Disfonksiyon Üzerine Etkisi

Öz

Amaç: Çalışmamızda fulvik asitin antioksidan özelliğinden yararlanarak erektil
disfonksiyon ile bağlantılı olan penil doku hasarın üzerine olan etkisini
amaçladık.

Gereç ve Yöntem:
Çalışmamızda 18 adet Sprague-Dawley (250-300gr) erişkin erkek sıçan 3 eşit
gruba ayrıldı: Kontrol (K), Kronik Stres (KS) ve Kronik Stres+Fulvik Asit
(KS+FA). Penis doku kesitleri Hematoksilen&Eozin (H&E) ve Masson
trikrom ile histokimyasal; endoteliyal; nöronal
nitrik oksit sentaz (eNOS;nNOS), Kaveloin-1, immünohistokimya işaretlemeler
incelenmiştir. Biyokimyasal olarak dokulardaki total antioksidan seviyesi
(TAS), total oksidan seviyesi (TOS), oksidatif stres indeksi (OSİ), katalaz
(KAT), glutatyon peroksidaz (GPx) ve süperoksit dismutaz (SOD) düzeyleri ölçülmüştür.

Bulgular: KS grubu
incelendiğinde
kontrol grubuna kıyasla trabeküler endotel
hücrelerinde kayıp, kollajen liflerde azalmalar ve incelmeler, bağ dokusu ve
düz kas dağılımında bozulmalar gözlenmiştir. KS+FA grubu incelendiğinde KS
grubuna kıyasla oluşan hasarlarda tam bir iyileşme gözlenmemekle beraber
dokularda yer yer düzelmeler rastlanmıştır. 
KS grubunda eNOS ve nNOS aktivitesinin kontrol grubuna göre azalmış,
kaveolin-1 reaksiyonun ise artmış olduğu gözlenmiştir. Aynı
grupta TOS ve OSI değerlerinin arttığı, TAS, SOD, KAT ve GPx değerlerinin de
azaldığı görülmüştür. KS+FA grubunda fulvik asit kullanımı ile kontrol grubuna
yakın olduğu gözlenmiştir.

Sonuç: Kullanılan fulvik asitin tedavi edici etkileri olduğu fakat
tedavi için kullanılan dozun yeterli miktarda olmadığı saptanmıştır. Tedavi
edici etkinin yeterli miktarda olması için farklı dozlarda çalışılmasını ileri
sürmekteyiz.

Anahtar Kelimeler

Erektil disfonksiyon, Sudan kaçınma stresi, Oksidatif stres, İnfertilite, Fulvik asit

Kaynakça

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