Influence of IAA, 28-homobrassinolide and 24-epibrassinolide on promoting grapevine adventitious rooting

Yıl 2018, Cilt: 6 Sayı: 1, 23 - 30, 17.07.2018

Zeliha Gökbayrak Ömer Uzunoğlu

Öz

In this research, influence of auxin and brassinosteroids on rooting of
American grapevine rootstocks (140 Ru and 41 B) was investigated. Hormones and
their concentrations used were indole butyric acid (IBA); 0 (control), 1000, 1500, 2000 and 4000 ppm for 5-second
dipping, 22(S),23(S)-homobrassinolide (HBR) and 24- epibrassinolide
(EBR); 0
(control), 10, 0,25, 0,50 and 1,00 ppm for 10-minute dipping. In the cuttings
allowed to grow and develop in a growth chamber were calculated firstly the
ratios of sprouting, rooting and healthy plant. Later, in the rooted and
sprouted cuttings, root development (root number, longest root length, root
development scale, fresh and dry root weights, dry root ratio) and shoot
development (primary shoot length, node number, shoot number, auxiliary shoot
number) were determined. 140 Ru had always better growth and development compared
to 41 B. Although IBA applications did not have distinctly significant effects
on root and shoot development of the two rootstocks, applications of 5 second
1500 ppm IBA and 10 minute 1.00 ppm HBR were shown to stimulate rooting and healthy
plant ratios in the rootstock 41 B. EBR, on the other hand, did not yield any
success. Along with 1000 ppm IBA, 0.50 and 1.00 ppm HBR, all EBR treatments
resulted in noticeable increase in the ratios of rooting and healthy plant in
140 Ru. This research showed that brassinosteroids might be used successfully
as auxins in inducing rooting in grapevine rootstocks cuttings.

Anahtar Kelimeler

auxin, IBA, brassinosteroid, homobrassinolide, epibrassinolide, grapevine, rooting

IAA, 28-homobrassinolid ve 24-epibrassinolidin asmanın adventif köklenmesinin teşviki üzerine etkisi

Öz

Bu araştırmada oksin ve brassinosteroidlerin
Amerikan asma anaçlarının (140 Ru ve 41 B) köklenmesi üzerine olan etkisi
araştırılmıştır. Kullanılan hormonlar ve konsantrasyonları şöyledir: indol
butirik asit (IBA) 0 (kontrol), 1000,
1500, 2000 ve 4000 ppm 5 saniyelik daldırma, 22(S),23(S)-homobrassinolid
(HBR)
ve 24-
epibrassinolid (EBR); 0 (kontrol), 10, 0,25, 0,50 ve 1,00 ppm 10
dakikalık daldırma. İklim odasında büyümesine ve gelişmesine izin verilen
çeliklerde önce sürme, köklenme ve sağlıklı bitki oranları hesaplanmıştır. Daha
sonra, köklenen ve süren çeliklerde kök gelişimi (kök sayısı, en uzun kök
uzunluğu, kök gelişimi derecesi, taze ve kuru kök ağırlıkları ile kuru kök
oranı) v sürgün gelişimi (primer sürgün uzunluğu, boğum sayısı, sürgün sayısı
ve koltuk sürgünü sayısı) belirlenmiştir. 140 Ru daima 41 B anacına göre daha
iyi büyüme ve gelişme göstermiştir. IBA uygulamalarının her iki anacın büyümesi
ve gelişmesi üzerine belirgin önemli etkileri tespit edilmemekle birlikte 5
saniye 1500 ppm IBA ve 10 dakika 1.00 ppm HBR uygulamaları 41 B anacında
köklenmeyi ve sağlıklı bitki oranlarını teşvik ettiği gösterilmiştir. Diğer
taraftan EBR herhangi bir başarı sağlamamıştır. 1000 ppm IBA, 0.50 ve 1.00 ppm
HBR ve tüm EBR uygulamaları 140 Ru anacında dikkati çeken bir köklenme ve sağlık
bitki oranı artışı vermiştir. Bu araştırma brassinosteroidlerin asma
çeliklerinin köklendirilmesinde başarılı şekilde kullanılabileceğini
göstermiştir.

Anahtar Kelimeler

oksin, IBA, brassinosteroid, homobrassinolid, epibrassinolid, asma, anaç

Kaynakça

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