Design of Reversed Phase Chromatography (RPC) Column Packing Material for Protein Separation: Part 1- Synthesis and Characterization

Yıl 2017, Cilt: 38 Sayı: 4, 788 - 797, 08.12.2017

Berna Saraçoğlu Kaya Ebru Sağ

https://doi.org/10.17776/csj.349325

Cited By: 1

Öz

In the first part of the two-part study, the column packed
materials in the form of monodisperse and porous particles were synthesized
with regard to test their column performances in Reverse Phase Chromatography
(RPC). Particles in the form of poly(vinyl acetate-co-divinylbenzene)
[poly(VAc-co-DVB)] and poly(divinylbenzene) [poly(DVB)] were produced by
“multi-stage micro-suspension polymerization”. The surface of particles were
converted to “hydroxyl” form by base hydrolysis that were used as
poly(vinylalcohol-co-divinylbenzene) [poly(VA-co-DVB)] particles, having
appropriate structural properties like surface and porosity for chromatographic
applications. Particle characterization was assessed by Scanning Electron
Microscopy (SEM), BET surface area and porosity measurement system and Fourier
Transform Infrared Spectroscopy (FTIR).

Anahtar Kelimeler

Monodisperse-Porous Particle, Multi-Stage Micro-Suspension Polymerization, Column Packed Material, Column Packed Material

Proteinleri Ayırmak İçin Ters Faz Kromatografisi (RPC) Kolon Dolgu Materyali Tasarımı: Bölüm 1- Sentez ve Karakterizasyon

Öz

İki bölümden oluşan çalışmanın ilk bölümünde
Ters Faz Kromatografisi (Reversed Phase Chromatography, RPC) için eş boyutlu ve
gözenekli partikül formunda poli(vinil asetat-co-divinilbenzen)
[poli(VAc-co-DVB)] ve poli(divinilbenzen) [poli(DVB)] kolon dolgu materyalleri
“Çok Basamaklı Mikrosüspansiyon Polimerizasyonu” yöntemi ile sentezlenmiştir.
Partiküller bazik ortamda hidrolizlenerek, partikül yüzeyi “hidroksil” formuna
dönüştürülmüş ve kromatografik kullanım için uygun yüzey ve gözenek yapısına
sahip poli(vinilalkol-co-divinilbenzen) [poli(VA-co-DVB)] partiküller elde
edilmiştir. Partiküllerin karakterizasyonu Taramalı Elektron Mikroskobu
(Scanning Electron Microscopy, SEM), yüzey alanı ve gözeneklilik ölçüm sistemi
(Brunauer–Emmett–Teller, BET) ve Fourier Transfer Infrared Spektrofotometre
(Fourier Transform Infrared Spectroscopy, FTIR) ile yapılmıştır.

Anahtar Kelimeler

Eşboyutlu-Gözenekli Partikül, Çok Basamaklı Mikrosüspansiyon Polimerizasyonu, Kolon Dolgu Materyali, Kolon Dolgu Materyali

Kaynakça

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