Synthesis, Characterization and In Vitro Antioxidant Activities of New Benzimidazole Ruthenium- (II) Complexes

Yıl 2017, Cilt: 38 Sayı: 4, 738 - 747, 08.12.2017

Serap Şahin Bölükbaşı Neslihan Şahin

https://doi.org/10.17776/csj.349326

Öz

In
this study; it was first intended to synthesize 1-substituted benzimidazole
ligands containing a nitrogen-bound bulky or functional group and then
preparing Ru- (II) -N coordinated metal complexes of these ligands. At last
stage of study it has been investigated that the antioxidant activities of
synthesized metal-complexes. Inert atmosphere-vacuum system has been used for the synthesis of all
complexes. Their structure were characterized by spectroscopic techniques. The in
vitro antioxidant activities of metal complexes were investigated based on the
scavenging of hydroxyl, superoxide and 2,2-diphenyl-1-picrylhydrazyl (DPPH)
radicals. In addition, total antioxidant activity of each metal-complex was
also determined. It was determined that total antioxidant activities
of metal-complexes increased by the order of 
3, 4 and 2. Because of decreasing IC₅₀ values indicates that
increasing antioxidant activity, DPPH radical scavenging activity of complex 2, which has lower IC₅₀ value,
was greater than complex 4. IC₅₀
values have not seen for complex 3
for DPPH. It was observed that hydroxyl radical scavenging activities of
complexes increased 4, 3 and 2, while superoxide
radical scavenging activities of complexes increased by the order of 3,
2 and 4, respectively.  As a result; newly
synthesized Ru (II) -azole complexes have been found to have significant
antioxidant activity against DPPH, hydroxyl and superoxide radicals.

Anahtar Kelimeler

Benzimidazole, Ruthenium, DPPH, Hydroxyl Radical, Superoxide Radical

Yeni Benzimidazol Rutenyum-(II) Komplekslerinin Sentezi, Karakterizasyonu ve İn Vitro Antioksidan Aktiviteleri

Öz

Bu
çalışmada; ilk olarak azota bağlı hacimli veya fonksiyonel grup içeren
1-sübstitüye benzimidazol ligantlarının sentezlenmesi
ve daha sonra sentezlenen bu ligantların Ru-(II)-N-koordine metal
komplekslerinin  hazırlanması
amaçlanmıştır. Son
aşamada ise sentezlenen metal-komplekslerin antioksidan aktiviteleri çalışılmıştır. Bileşeklerin sentezlenmesinde inert atmosfer-vakum
sistemi kullanılmıştır. Tüm komplekslerin 
yapıları spektroskopik tekniklerle aydınlatılmıştır. Sentezlenen metal
komplekslerin in vitro antioksidan
aktiviteleri; hidroksil, süperoksit ve DPPH radikallerini süpürme özellikleri
ile araştırılmıştır. Ayrıca her bir metal-kompleksin toplam antioksidan
aktivitesi de saptanmıştır. Metal-komplekslerin toplam antioksidan aktivitelerinin 3, 4 ve 2 sıralamasıyla arttığı saptanmıştır. Azalan IC₅₀ değeri
artan antioksidan aktiviteye işaret ettiğinden, daha düşük IC₅₀ değerine
sahip olan 2 numaralı bileşiğin DPPH
radikali süpürme aktivitesinin 4
numaralı bileşikten daha yüksek olduğu bulunmuştur. DPPH radikali süpürme
aktivitesinde, 3 numaralı bileşik
için IC₅₀ değeri gözlenmemiştir. Bileşiklerin hidroksil radikali
süpürme aktivitelerinin 4, 3 ve 2 sıralamasıyla artarken, süperoksit
radikali süpürme aktivitelerinin ise; 3,
2 ve 4 sıralamasıyla arttığı
bulunmuştur. Sonuç olarak; yeni
sentezlenen Ru(II)-azol komplekslerinin DPPH, hidroksil ve süperoksit
radikallerine karşı önemli antioksidan aktiviteye sahip oldukları bulunmuştur.

Anahtar Kelimeler

Benzimidazol, Rutenyum, DPPH, Hidroksil Radikali, Süperoksit Radikali

Kaynakça

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