Sivas’ta Kuşburnu (Rosa canina L.)’nda Zarar Yapan Diplolepis fructuum (Rübsaamen 1895) (Hymenoptera: Cynipoidea)’un Parazitoitleri ve Parazitoitlik Durumlarının Belirlenmesi

Yıl 2017, Cilt: 38 Sayı: 4, 699 - 710, 08.12.2017

Lütfiye Gençer Merve Gül

https://doi.org/10.17776/csj.362657

Cited By: 2

Öz

Bu
çalışma kuşburnu bitkisinde gal oluşturan Diplolepis fructuum’ un
(Hymenoptera: Cynipoidea)'un parazitoitleri ve bu parazitoitlerin,
parazitoitlik durumlarını belirlemek için yapılmıştır. Çalışma sonucu
parazitoit olarak Chalcidoidea (Hymenoptera) üstfamilyasına ait 13 tür tespit
edilmiştir. Parazitoitlerin teşhis edilmesinde iki yol izlenmiştir. Bunlardan
ilki ergin ve larva üzerinden morfolojik incelemelerle yapılan teşhistir.
İkincisi morfolojik olarak değerlendirilen ergin ve larvalarının genomik DNA
izolasyonu yapılıp, çekirdek (ITS2) ve mitokondri (COI) genomlarına ait
belirteçler dizilenerek dizi bilgileri karşılaştırılmıştır. Ayrıca açılan
gallerin incelenmesi sonucu parazitoitlerin tamamının ektoparazitoit yaşam
stratejisine sahip oldukları tespit edilmiştir. 

Anahtar Kelimeler

Diplolepis fructuum, ITS2, Parazitoit, Rosa canina, Total çekirdek genomu

Determination of Parasitoids and its Parasitoids Life of Diplolepis fructuum (Rübsaamen) (Hymenoptera: Cynipoidea) as Pest of Rosehip (Rosa canina) in Sivas

Öz

This
study was made to determine parasitoids and their parasitoids life of D.
fructuum which are gall-inducing on Rosa canina. In the results of
the study, as parasitoids were recorded thirteen species belong to Chalcidoidea
superfamily. Identification of parasitoids were observed in two ways. The first
of these,  made by morphological
examination of adult and larva. Second, it was done by genomic DNA isolation of
adult and larva evaluated morphologically and was compared by sequencing of
nuclear (ITS2) and mitochondrial (COI) genome. In addition, examination of
opened gall were determined as ectoparasitoid of life strategy of all
parasitoids. 

Anahtar Kelimeler

Diplolepis fructuum, ITS2, Parasitoids, Rosa canina, Total Core Genome

Kaynakça

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