Electrochemical Behavior of Cholecalciferol on a Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode

Yıl 2018, Cilt: 39 Sayı: 4, 1081 - 1088, 24.12.2018

Şerife Eryılmaz Kahya Mustafa Cittan Ali Çelik

https://doi.org/10.17776/csj.439630

Cited By: 9

Öz

Herein,
electrochemical behavior of cholecalciferol on a multiwalled carbon nanotube
modified glassy carbon electrode was evaluated by using cyclic voltammetry.
Voltammetric determination of cholecalciferol on the prepared modified
electrode was carried out using linear sweep voltammetry. A good linearity was
obtained with a correlation coefficient of 0.9914 between 5×10^-5 – 1×10^-3 M. The LOD and LOQ values were
calculated as 1.7×10^-5
and 5.1×10^-5 M, respectively. The response of the proposed electrode was
sufficiently repeatable for determining cholecalciferol. Finally, the proposed modified
electrode was successfully applied to the determination of cholecalciferol in a
commercial oral solution that contains 300.000 I.U cholecalciferol/mL. A simple
liquid-liquid extraction technique by using methanol was followed to extract cholecalciferol from the oral liquid. The
results obtained with the proposed method were in agreement with
cholecalciferol content of the commercial oral solution.

Anahtar Kelimeler

Cholecalciferol, Multiwalled carbon nanotube, Voltammetry

Çok Duvarlı Karbon Nanotüp Modifiye Camsı Karbon Elektrot Üzerinde Kolekalsiferolün Elektrokimyasal Davranışı

Öz

Bu çalışmada,
kolekalsiferolün çok duvarlı karbon nanotüp modifiye camsı karbon elektrot
üzerindeki elektrokimyasal davranışı, döngüsel voltammetri yöntemi kullanılarak
değerlendirilmiştir. Hazırlanan modifiye elektrot yüzeyinde kolekalsiferolün
voltammetrik tayini, doğrusal taramalı voltammetri yöntemi kullanılarak
gerçekleştirilmiştir. 5×10^-5– 1x10^-3 M konsantrasyon aralığında
0.9914 korelasyon katsayısı ile iyi bir doğrusallık elde edilmiştir. LOD ve LOQ
değerleri sırasıyla 1.7×10^-5 ve 5.1×10^-5 M olarak
hesaplanmıştır. Önerilen elektrodun cevabı kolekalsiferolün belirlenmesi için
yeterince tekrarlanabilirdir. Önerilen modifiye elektrot, 300.000 I.U kolekalsiferol/mL
içeren ticari bir oral solüsyonda kolekalsiferol tayini için başarıyla
uygulanmıştır. Oral solüsyondan kolekalsiferolü ekstrakte etmek için metanol
kullanılarak gerçekleştirilen basit bir sıvı-sıvı ekstraksiyon tekniği
izlenmiştir. Önerilen yöntem ile elde edilen sonuçlar, ticari oral solüsyonun
kolekalsiferol içeriği ile uyum içerisindedir.

Anahtar Kelimeler

Kolekalsiferol, Çok duvarlı karbon nanotüp, Voltammetri

Kaynakça

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