Fonofos’un Zebra Balığı (Danio rerio) Solungaçlarındaki Histopatolojik Etkileri

Yıl 2019, Cilt: 40 Sayı: 1, 35 - 41, 22.03.2019

Sezgi Arman Sema İşisağ Üçüncü

https://doi.org/10.17776/csj.424499

Öz

Organofosfatlı pestisitler dünya çapında tarımsal verimliliği artırmak
için yaygın olarak kullanılmaktadır. Ancak, bu kimyasalların genel olarak hedef
özgüllüğü göstermediği ve hedef dışındaki omurgalı canlıların da pestisit
maruziyetinden etkilendikleri ortaya konmuştur. Bu çalışmanın amacı,
organofosfatlı bir insektisit olan fonofosun zebra balığının (Danio rerio) solungaç dokularındaki
potansiyel zararlı etkilerini araştırmaktır. Ergin zebra balıkları 96 saat
boyunca 1, 2 ve 4 mg/L fonofosa maruz bırakıldı. Solungaçlar çıkartılarak Bouin
sıvısında tespit edildi ve parafine gömüldü. 5 µm kalınlıktaki kesitler
hematoksilen eozin, Gomori trikrom veya periyodik asit Schiff boyaları ile
boyandı. Örnekler ışık mikroskobunda incelenerek histolojik değişimleri %0,1
dimetilsülfoksit (DMSO) uygulanan çözücü kontrol grubu örnekleriyle
karşılaştırıldı. Fonofos solungaçlarda, epitel dokuda kalkma, sekonder
lamellerde kıvrılma, deskuamasyon, epitelyal hiperplazi ve lamellar füzyona yol
açtı. Solungaçlar fonofos maruziyeti ile belirgin histopatolojik farklılıklar
gösterdi. Fonofosun sucul ekosistemlerde yaşayan hedef dışı organizmalar için
bir tehdit oluşturduğu belirlenmiştir.

Anahtar Kelimeler

Fonofos, insektisit, solungaç, histopatoloji, zebra balığı, Danio rerio

Histopathological Effects of Fonofos on Gills of Zebrafish (Danio rerio)

Öz

Organophosphate pesticides are commonly used to increase agricultural
efficiency worldwide. However, it has been proved that these chemicals
generally do not show target specificity and non-target vertebrate organisms
are adversely affected by pesticide exposure. The aim of the present study was
to examine the potential harmful effects of fonofos, an organophosphate
insecticide, on the gill tissues of zebrafish (Danio rerio). Adult zebrafish were exposed to 1, 2 and 4 mg/L
fonofos for 96 hours. Gill tissues were removed, fixed with Bouin’s fluid and
embedded in paraffin. 5 µm serial sections were stained with hematoxylin-eosin,
Gomori trichrome or periodic acid-Schiff. Samples were investigated by light
microscopy and histological alterations were compared with the 0.1% dimethyl
sulfoxide (DMSO) exposed solvent control samples. Fonofos caused epithelial
lifting, curling of secondary lamellae, desquamation, epithelial hyperplasia,
and lamellar fusion in the gills. Gills showed distinct histopathological
changes through fonofos exposure. It was determined that fonofos is a threat to
non-target organisms living in aquatic ecosystems.

Anahtar Kelimeler

Fonofos, insecticide, gill, histopathology, zebrafish, Danio rerio

Kaynakça

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