DNA Biyosensör Uygulamaları İçin Karbon Nanotüp Modifiye Yüzeylerin Ferrisiyanür ve Guanin Sinyallerine Dayalı Olarak Elektrokimyasal İncelenmesi

Yıl 2019, Cilt: 40 Sayı: 1, 11 - 23, 22.03.2019

Dilşat Arıksoysal

https://doi.org/10.17776/csj.441382

Öz

Bu
çalışmada karbon nanotüpler (CNT) ile modifiye edilmiş karbon pastası (CPE) ve
laboratuvar koşullarında basılarak üretilen perde baskılı karbon (SPE)
elektrotların performansı karşılaştırılmış ve dönüşümlü voltametri(CV), kare
dalga voltametri (SWV) veya diferansiyel puls voltametri (DPV) teknikleri
kullanılarak elde edilen potasyum ferri / ferrosiyanür veya guanin
sinyallerindeki artış miktarı tayin edilmiştir. Bu çalışmada elektrotlara
kimyasal (H₂SO₄, aseton, N, N-Dimetilformamid veya NaOH)
veya elektrokimyasal (farklı potansiyel uygulamaları) gibi farklı aktivasyon
prosedürleri uygulanmıştır. Nanotüp modifiye elektrota uygulanan aktivasyon
prosedürünün sinyal zenginleşmesi üzerine güçlü etkileri olduğu gözlenmiştir.
Bu prosedürlerden NaOH ile aktivasyonda elde edilen guanin sinyalinin yaklaşık
62 kat arttığı tespit edilmiştir. Bu çalışmada ayrıca farklı nanotüp türlerinin
aktivasyon proseslerine farklı yanıtlar verdiği de bulunmuştur. Nanotüp bazlı
biyosensörün optimum şartları da ayrıca sunulmuştur.

Anahtar Kelimeler

Elektrokimyasal teknikler, DNA, biyosensör, karbon nanotüpler, perde baskılı elektrot (SPE), Nükleik asit hibridizasyonu

Electrochemical Investigation of Carbon Nanotube Modified Surfaces Based on Ferricyanide and Guanine Signals for DNA Biosensor Applications

Öz

This
study was designed to investigate the performance of carbon nanotubes (CNT)
modified carbon paste and carbon printed electrodes (SPE) produced in
laboratory conditions. The effect of carbon nanotube use on signal enrichment
was determined by using cyclic voltammetry (CV), square wave voltammetry (SWV)
or differential pulse voltammetry (DPV) techniques based on potassium
ferricyanide/ ferrocyanide or guanine signal. The application of different
activation procedures to the electrode surface such as chemical (H₂SO₄,
acetone, N,N-Dimethylformamide or NaOH) or electrochemical (different potential
applications) were presented in this study. It was observed that the activation
procedure applied to the nanotube modified electrode has strong effects on
signal enrichment. From these procedures it was determined that the guanine
signal obtained in activation with NaOH increased about 62-fold. It was also
found that different nanotube species gave different responses to the
activation processes. The optimum conditions of the nanotube-based biosensor
were also presented.

Anahtar Kelimeler

Electrochemical techniques, DNA, biosensor, carbon nanotubes, screen printed electrode(SPE), Nucleic Acid Hybridization

Kaynakça

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