Genoprotective Effects of Aqueous Extracts of Rosa Canina L. Fruits on Ethyl Methanesulfonate-Induced DNA Damage in Drosophila Melanogaster

Yıl 2016, Cilt: 37 Sayı: 3, 241 - 247, 16.09.2016

Caner Kasımoğlu Handan Uysal

https://doi.org/10.17776/csj.18487

Cited By: 1

Öz

Abstract. In this study, the possible genotoxic effects of ethyl methanesulfonate (EMS) which are one of alkylating agent and genoprotective effects of Rosa canina L. fruit water extract (RC_wtr) was studied with Drosophila wing somatic mutation and recombination test (SMART). Five different application groups (distilled water, 1 mM EMS, 1% RC_wtr, 3% RC_wtr and 5% RC_wtr) were formed with preliminary studies. 3-day-old transheterozygous larvae of mwh/flr³ genotype of Drosophila melanogaster were fed chronically on the Drosophila instant medium (DIM) including the application groups. The wing slides of normal wing (mwh/flr³) phenotype individuals were prepared and examined under light microscope (400X).

As a result of experiments, the total clone frequencies of distilled water control group, 1 mM EMS, 1% RC_wtr, 3% RC_wtr and 5% RC_wtr application groups were determined as 0.15, 3.55, 2.58, 2.78 and 2.20, respectively. The difference between distilled water control group and 1 mM EMS application group is statistically significant (P<0.05). According to the results obtained from RC_wtr application groups, each group’s total clon frequencies decreased compared with 1 mM EMS application group. It was found that the differences between the 1mM EMS and RC_wtr. application groups were statistically important too (P<0.05). The findings demonstrate that the constituents of Rosa canina L. have great potential as a natural genoprotective product.

Keywords: Rosa canina, SMART, Drosophila melanogaster, Ethyl methanesulfonate, genoprotective

 

Özet. Bu çalışmada, alkilleyici ajanlardan birisi olan etil metansülfonatın olası genotoksik etkileri ve Rosa canina L. meyvelerinin su ekstraktının (RC_su) genomik koruyucu etkisi Drosophila kanat somatik mutasyon ve rekombinasyon testi (SMART) ile araştırılmıştır. Yapılan ön çalışmalar ile beş farklı uygulama grubu (saf su, 1 mM EMS, 1% RC_su, 3%  RC_su ve 5% RC_su) hazırlanmıştır. Drosophila melanogaster’in mwh/flr³ genotipli 3. evre trans-heterozigot larvaları, uygulama gruplarını içeren hazır Drosophila besiyerinde kronik olarak beslenmiştir. Normal kanat fenotipli (mwh/flr³) bireylerin kanat preparatları hazırlanmış ve ışık mikroskobunda incelenmiştir (400X).

Deneylerin sonucunda saf su kontrol grubu, 1 mM EMS, %1 RC_su, %3 RC_su ve %5 RC_su uygulama gruplarının toplam klon frekansları sırasıyla 0.15, 3.55, 2.58, 2.78 ve 2.20 olarak belirlenmiştir. Saf su kontrol grubu ve 1 mM EMS uygulama grupları arasındaki fark istatistiksel olarak önemlidir (P<0.05). RC_su uygulama gruplarından elde edilen sonuçlara göre ise, 1 mM EMS uygulama grubu ile karşılaştırıldığında her grubun toplam klon frekansı azalmıştır. 1mM EMS ve RC_su uygulama grupları arasındaki farkların istatistiksel olarak oldukça önemli olduğu bulunmuştur (P<0.05). Bulgular, Rosa canina L. bileşenlerinin doğal bir genomik koruyucu ürün olarak büyük bir potansiyele sahip olduğunu göstermektedir.

Anahtar kelimeler: Paraben, Rosa canina, SMART, Drosophila melanogaster, Etil metansülfonat, Genomik koruyucu

Ethyl Methanesulfonate-Induced DNA Damage in Drosophila Melanogaster

Öz

Deneylerin sonucunda saf su kontrol grubu, 1 mM EMS, %1 RCsu, %3 RCsu ve %5 RCsu uygulama gruplarının toplam klon frekansları sırasıyla 0.15, 3.55, 2.58, 2.78 ve 2.20 olarak belirlenmiştir. Saf su kontrol grubu ve 1 mM EMS uygulama grupları arasındaki fark istatistiksel olarak önemlidir (P

Anahtar Kelimeler

Paraben, Rosa canina, SMART, Drosophila melanogaster, Etil metansülfonat, Genomik koruyucu

Kaynakça

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