Aeromonas Septicemia Infection and Histopathologic Findings in Cultured Narrow-Clawed Crayfish (Pontastacus leptodactylus, Eschscholtz, 1823)

Year 2024, Volume: 20 Issue: 2, 89 - 96, 01.06.2024

Şeydanur Kan Pınar Yıldırım Özlem Özmen Seval Bahadır Koca Ayşegül Kubilay

https://doi.org/10.22392/actaquatr.1298890

Abstract

In this study, bacterial, parasitic, and fungal analyses were carried out on 15 individuals showing disease symptoms in crayfish (Pontastacus leptodactylus). Samples were collected from internal organs for pathological examinations. As a result of the pathological examinations, no parasitic and fungal pathogens agents were found. Samplings were gathered from the hepatopancreas and streaked onto tryptic soy agar (TSA) 96 and consequently, a pure bacterial colony was obtained. As a result of the bacterial tests, the bacteria were determined to be Aeromonas sp. genus. Narrow-clawed crayfish showed 100% mortality within 21 days of disease outbreak. Histopathologically, significant changes were observed in hepatopancreas and intestinal tissues. In hepatopancreatic tissue, prominent pathological findings characterized by prominent vacuolization in the epithelium, irregular tubules, proliferation of epithelial cells, nuclear pycnosis, and inflammatory cell infiltrations were detected. The findings of this study demonstrated that Aeromonas sp. caused important pathological findings in narrow-clawed crayfish.

Keywords

Aeromonas sp., Narrow-Clawed Crayfish, İnfection, Histopathology.

Kültür Ortamında Yetiştiriciliği Yapılan Tatlısu Istakozu (Pontastacus leptodactylus, Eschscholtz, 1823)'nda Hareketli Aeromonas Septisemi ve Histopatolojik Bulgular

Abstract

Bu çalışmada yetiştiriciliği yapılan kerevitlerde (Pontastacus leptodactylus) hastalık belirtileri gösteren 15 adet bireyde bakteriyel, paraziter ve fungal incelemeler yapılmış; patolojik incelemeler için iç organlardan örnek alınmıştır. İncelemeler neticesinde paraziter ve fungal patojene rastlanılmamıştır. Kerevitlerin hepatopankreasından Triptik Soy Agar (TSA) besiyerine yapılan bakteriyolojik ekimden saf bakteri kolonisi izole edilmiştir. Yapılan testler sonucunda bakterinin Aeromonas sp. cinsine ait olduğu belirlenmiştir. Kerevitlerde hastalık çıktıktan sonraki 21 gün içinde %100 ölüm görülmüştür. Histopatolojik olarak; hepatopankreas ve bağırsak dokularında belirgin değişiklikler olduğu gözlemlenmiştir. Hepatopankreatik dokuda; epitellerde belirgin vakuolizasyon, tübüllerde düzensizlik, epitel hücrelerinde proliferasyon, nükleer piknoz ve inflamatuar hücre infiltrasyonları ile karakterize edilen belirgin patolojik bulgular tespit edilmiştir. Bu çalışmanın bulguları Aeromonas sp.’nin kerevitlerde önemli patolojik bulgular oluşturduğunu göstermiştir.

Keywords

Aeromonas sp., Kerevit, Enfeksiyon, Histopatoloji.

References

• Austin, B., & Austin, D. A. (2012). Bacterial Fish Pathogens. Diseases of Farmed and Wild Fish, 5th ed. Springer.
• Avsever, M. L., Hilmioğlu Polat, S., Türk, N., & Metin, D. Y. (2011). Tatlı su istakozlarından (Astacus leptodactylus) Saprolegnia sp. ve Aeromonas hydrophila izolasyonu. Kafkas Üniviversitesi Veterinerlik Fakültesi Dergisi, 17(5), 873-875.https://doi.org/10.9775/Kvfd.2011.4714
• Buller, N. B. (2004). Bacteria from fish and other aquatic animals: a practical identification manual. https://doi.org/10.1079/9780851997384.0000
• Cascón, A., Fregeneda, J., Aller, M., Yugueros, J., Temprano, A., Hernanz, C., Sánchez, M., Rodríguez‐Aparicio, L., & Naharro, G. (2000). Cloning, characterization, and insertional inactivation of a major extracellular serine protease gene with elastolytic activity from Aeromonas hydrophila. Journal of Fish Diseases, 23(1), 49–59. https://doi.org/10.1046/j.1365-2761.2000.00206.x
• Cerenius, L., & Söderhäll, K. (2018). Crayfish immunity – Recent findings. Developmental &ComparativeImmunology, 80, 94–98. https://doi.org/10.1016/j.dci.2017.05.010
• Chinain, M., & Vey, A. (1988). Experimental study of Fusarium solani: Infections in Astacus leptodactylus and Pacifastacus leniusculus (Crustacea, Decapoda). Diseases of Aquatic Organisms, 5, 215–223. https://doi.org/10.3354/dao005215
• Corbel, V., Zuprizal, Z., Shi, C., Huang, Sumartono, Arcier, J.-M., & Bonami, J.-R. (2001). Experimental infection of European crustaceans with white spot syndrome virus (WSSV). Journal of Fish Diseases, 24(7), 377–382. https://doi.org/10.1046/j.1365-2761.2001.00302.x
• Crandall, K. A., & De Grave, S. (2017). An updated classification of the freshwater crayfishes (Decapoda: Astacidea) of the world, with a complete species list. Journal of Crustacean Biology, 37(5), 615–653. https://doi.org/10.1093/jcbiol/rux070
• Diler, O., & Bolat, Y. (2001). Isolation of Acremonium species from crayfish, Astacus leptodactylus in Egirdir Lake. Bulletin-European Association of Fish Pathologists, 21(4), 164-168.
• Dobrzycka-Krahel, A., & Fidalgo, M. L. (2023). Euryhalinity and Geographical Origin Aid Global Alien Crayfish Invasions. Water, 15(3), 569. https://doi.org/10.3390/ w15030569
• Dragičević, P., Bielen, A., Petrić, I., & Hudina, S. (2020). Microbial pathogens of freshwater crayfish: A critical review and systematization of the existing data with directions for future research. Journal of Fish Diseases, 44(3), 221–247. https://doi.org/10.1111/jfd. 13314
• Farmer, J. J., Arduino, M. J., & Hickman-Brenner, F. W. (2006). The Genera Aeromonas and Plesiomonas. In M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer, E. Stackebrandt, (Eds). The Prokaryotes (pp. 564–596). Springer. https://doi.org/10. 1007/0-387-30746-x_19
• Halder, S. K., Maity, C., Jana, A., Ghosh, K., Das, A., Paul, T., Mohapatra, P. K. D., Pati, B. R., & Mondal, K. C. (2014). Chitinases biosynthesis by immobilized Aeromonas hydrophila SBK1 by prawn shells valorization and application of enzyme cocktail for fungal protoplast preparation. Journal of Bioscience and Bioengineering, 117(2), 170–177. https://doi.org/10.1016/j.jbiosc.2013.07.011
• Hayakijkosol, O., Owens, L., & Picard, J. (2017). Case report of bacterial infections in a redclaw crayfish (Cherax quadricarinatus) hatchery. Aquaculture, 475, 1–7. https://doi.org/10.1016/j.aquaculture.2017.03.038
• Holt, J. G. (1977). The shorter Bergey's manual of determinative bacteriology. Williams & Wilkins Co.
• Jiravanichpaisal, P., Roos, S., Edsman, L., Liu, H., & Söderhäll, K. (2009). A highly virulent pathogen, Aeromonas hydrophila, from the freshwater crayfish Pacifastacus leniusculus. Journal of Invertebrate Pathology, 101(1), 56–66. https://doi.org/10.1016/j.jip.2009.02.002
• Khalil, R. H., El-Banna, S. A., & Mahfouz, N. B. (2009). Study on micro flora of wild freshwater crayfish (Procambarus clarkii). In Proceedings of the 2nd Global Fisheries and Aquaculture Research Conference, Cairo International Convention Center, 24-26 October 2009 (pp. 257-261). Massive Conferences and Trade Fairs.
• Li, C., Wang, R., Su, B., Luo, Y., Terhune, J., Beck, B., & Peatman, E. (2013). Evasion of mucosal defenses during Aeromonas hydrophila infection of channel catfish (Ictalurus punctatus) skin. Developmental &; Comparative Immunology, 39(4), 447–455. https://doi.org/10.1016/j.dci.2012.11.009
• Loker, E. S., Adema, C. M., Zhang, S.-M., & Kepler, T. B. (2004). Invertebrate immune systems - not homogeneous, not simple, not well understood. Immunological Reviews, 198(1), 10–24. https://doi.org/10.1111/j.0105-2896.2004.0117.x
• Maestracci, V., & Vey, A. (1988). Fungal infection of gills in crayfish: Histological, cytological and physiopathological aspects of the disease. Freshwater Crayfish, 7, 187–194. https://doi.org/10.5869/fc.1988.v7.187
• Michael, J. J., & Duffey, P. S. (1988). Mesophilic aeromonads in human disease: Current taxonomy, laboratory identification, and infectious disease spectrum. Reviews of Infectious Diseases, 10, 980–997
• OIE (Office International des Epizooties). (2003). Manual of diagnostic tests for aquatic animals, 4th ed. Office International des Epizooties.
• Özdemir, F., & Arslan, S. (2021). Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi, 2(1), 13-23.
• Raissy, M., Khamesipour, F., Rahimi, E., & Khodadoostan, A. (2014). Occurrence of Vibrio spp., Aeromonas hydrophila, Escherichia coli and Campylobacter spp. in crayfish (Astacus leptodactylus) from Iran. Iranian Journal of Fisheries Sciences, 13(4), 944-954.
• Rasmussen-Ivey, C. R., Figueras, M. J., McGarey, D., & Liles, M. R. (2016). Virulence Factors of Aeromonas hydrophila: In the Wake of Reclassification. Frontiers in Microbiology, 7, 1337. https://doi.org/10.3389/fmicb.2016.01337
• Raj, N. S., Swaminathan, T. R., Dharmaratnam, A., Raja, S. A., Ramraj, D., Lal, K. K., (2019).
• Aeromonas veronii caused bilateral exophthalmia and mass mortality in cultured Nile tilapia, Oreochromis niloticus (L.) in India. Aquaculture 512, 734278. https://doi.org/10.1016/j.aquaculture. 2019.734278
• Palu, A. P., Gomes, L. M., Miguel, M., Balassiano, I. T., Queiroz, M., Freitas-Almeida, A. C., & Oliveira, S. (2006). Antimicrobial resistance in food and clinical Aeromonas isolates. Food Microbiology, 23, 504–509. https://doi.org/10.1016/j.fm.2005.07.002
• Salighehzadeh, R., Sharifiyazdi, H., Akhlaghi, M., Khalafian, M., Gholamhosseini, A., & Soltanian, S. (2019). Molecular and clinical evidence of Aeromonas hydrophila and Fusarium solani co-infection in narrow-clawed crayfish Astacus leptodactylus. Diseases of Aquatic Organisms, 132(2), 135–141. https://doi.org/10.3354/dao03309
• SamCookiyaei, A., Afsharnasab, M., Razavilar, V., Motalebi, A. A., Kakoolaki, S., Asadpor, Y., Yahyazade, M. Y., & Nekuie Fard, A. (2012). Experimentally pathogenesis of Aeromonas hydrophila in freshwater Crayfish (Astacus leptodactylus) in iran. Iranian journal of fisheries sciences, 11(3), 644-656.
• Sun, S., Qin, J., Yu, N., Ge, X., Jiang, H., & Chen, L. (2013). Effect of dietary copper on the growth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis. Fish & Shellfish Immunology, 34(5), 1195–1201. https://doi.org/10.1016/j.fsi.2013.01.021
• Tomás, J. M. (2012). The Main Aeromonas Pathogenic Factors. ISRN Microbiology, 2012, 256261. https://doi.org/10.5402/2012/256261
• Yuan, G., Zhu, L., Jiang, X., Zhang, J., Pei, C., Zhao, X., Li, L., & Kong, X. (2021). Diagnosis of co-infection with white spot syndrome virus and Aeromonas veronii in red swamp crayfish Procambarus clarkii. Aquaculture, 532, 736010. https://doi.org/10.1016 /j.aquaculture.2020.736010