Evaluation of Biocompatibility Properties of Dental Materials: xCELLigence® System

Year 2024, Volume: 13 Issue: 2, 395 - 401, 20.05.2024

Makbule Buse Dundar Sarı

https://doi.org/10.54617/adoklinikbilimler.1401043

Abstract

The toxic and biological impacts of dental materials play a pivotal role in their clinical application within dentistry. The assessment of these materials typically commences with in vitro tests upon initial development, progressing to in vivo animal experiments and clinical trials. In vitro cell culture tests afford the examination of tissue responses at the cellular level, allowing the observation of physiological activities. Moreover, these tests offer a cost-effective and time-efficient alternative to animal experiments, rendering them easily applicable and replicable. Recently, real-time cell analysis systems, such as the xCELLigence® system, have emerged as a promising substitute for traditional testing methods, potentially surpassing them in the biocompatibility evaluation of dental materials. The xCELLigence® system facilitates the concurrent observation and analysis of cells within their authentic environment, obviating the need for cell staining or marking. This review seeks to underscore the advantageous features of the xCELLigence® system, which serves to mitigate the drawbacks associated with conventional in vitro biocompatibility evaluation methods.

Keywords

Animals, Biocompatibility, Clinical trials, Cytotoxicity tests, Dentistry, Materials testing

Dental Materyallerin Biyouyumluluk Özelliklerinin Değerlendirilmesi: xCELLigence® Sistemi

Abstract

Diş hekimliğinde dental materyallerin toksik ve biyolojik etkileri klinik kullanımda büyük bir öneme sahiptir. Dental materyaller yeni geliştirildiğinde canlı dokulardaki etkisi, etik ve yasal yükümlülükler nedeniyle öncelikle in vitro testler sonrasında in vivo hayvan deneyleri ve klinik deneyler ile değerlendirilmektedir. İn vitro hücre kültürü testleri ile dokuların hücre düzeyinde yanıtları incelenebilmekte ve fizyolojik aktiviteleri taklit edilebilmektedir. Ayrıca hücre kültürü testlerinin hayvan deneylerine göre maliyeti daha düşüktür. Daha kısa süre almakta, kolaylıkla uygulanabilmekte ve tekrar edilebilmektedir. Ancak gelişen ve değişen teknolojiyle birlikte geleneksel test yöntemlerine bir alternatif olan gerçek zamanlı hücre analiz sistemleri (xCELLigence® sistemi), dental materyallerin biyouyumluluk değerlendirmelerinde tercih edilebilir. Bu sistem ile hücrelerin boyanmasına veya işaretlenmesine gerek duyulmadan, hücreleri kendi gerçek ortamlarında gerçeğe eş zamanlı olarak gözlemlemek ve analiz etmek mümkündür. Bu derleme geleneksel in vitro biyouyumluluk değerlendirme yöntemlerinin dezavantajlarını elimine eden xCELLigence® sisteminin üstün özelliklerini vurgulamayı amaçlamaktadır.

Keywords

Biyouyumluluk, Diş hekimliği, Hayvanlar, Klinik denemeler, Materyal testi, Sitotoksisite testleri

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