Research Article
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Year 2024, Volume: 7 Issue: 3, 298 - 304, 15.05.2024
https://doi.org/10.47115/bsagriculture.1450790

Abstract

References

  • Abdul Kayum M, Nath UK, Park JI, Biswas MK, Choi EK, Song JY, Kim HT, Nou IS. 2018. Genome-wide identification, characterization, and expression profiling of glutathione S-transferase (GST) family in pumpkin reveals likely role in cold-stress tolerance. Genes, 9(2): 84.
  • Ambachew D, Asfaw A, Blair MW. 2023. Phenotypic variability for root traits in andean common beans grown with and without aluminum stress conditions. Agronomy, 13: 619.
  • Ambachew D, Blair MW. 2021. Genome wide association mapping of root traits in the andean gene pool of common bean (Phaseolus vulgaris L.) grown with and without aluminum toxicity. Front Plant Sci, 12: 628687.
  • Baloch FS, Nadeem MA. 2022. Unlocking the genomic regions associated with seed protein contents in Turkish common bean germplasm through genome-wide association study. Turk J Agric For, 46(1): 113-128.
  • Bartoli G, Sanità di Toppi L, Andreucci A, Ruffini Castiglione, M. 2017. Aluminum effects on embryo suspensor polytene chromosomes of Phaseolus coccineus L. Plant Biosys, 152(4): 880-888
  • Butare L, Rao IM, Lepoivre P, Cajiao C, Polania J, Cuasquer J, Beebe S. 2012. Phenotypic evaluation of interspecific recombinant inbred lines (RILs) of Phaseolus species for aluminium resistance and shoot and root growth response to aluminium-toxic acid soil. Euphytica, 186: 715-730.
  • Cançado GMA, DeRosa VE, Fernandez JH, Maron LG, Jorge RA, Menossi M. 2005. Glutathione S-transferase and aluminum toxicity in maize. Funct Plant Biol, 32: 1045-1055.
  • Çelik A, Emiralioğlu O, Yeken MZ, Çiftçi V, Özer G, Kim Y, Baloch FS, Chung YS. 2023. A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris. Front Genet, 14: 1136794.
  • Delhaize E, Ryan PR, Randall P. 1993. Aluminium tolerance in wheat (Triticum aestivum L.) II. Aluminium-stimulated excretion of malic acid from root apices. Plant Physiol, 103: 695-702.
  • Dmitriev AA, Krasnov GS, Rozhmina TA, Kishlyan NV, Zyablitsin AV, Sadritdinova AF, Snezhkina AV, Fedorova MS, Yurkevich OY, Muravenko OV, Bolsheva NL, Kudryavtseva AV, Melnikova NV. 2016. Glutathione S-transferases and UDP-glycosyltransferases are involved in response to aluminum stress in flax. Front Plant Sci, 7: 1920.
  • dos Santos Neto J, Delfini J, Willian ST, Akihide HA, Marcos NJ, Simões Azeredo Gonçalves L, Moda-Cirino V. 2020 Response of common bean cultivars and lines to aluminum toxicity. Agronomy, 10: 296
  • Eticha D, Zahn M, Bremer M, Yang Z, Rangel AF, Rao IM, Horst WJ. 2010. Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaseolus vulgaris) genotypes. Ann Bot, 105(7): 1119-1128.
  • Ezaki B, Suzuki M, Motoda H, Kawamura M, Nakashima S, Matsumoto H. 2004. Mechanism of gene expression of Arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress. Plant Physiol, 134: 1672-1682.
  • FAO. 2023. Food and Agriculture Statistics. Retrieved May 20, 2023 from: https://www.fao.org/faostat/en/#home (accessed date: June 15, 2023).
  • Ikegawa H, Yamamoto Y, Matsumoto H. 2000. Responses to aluminium of suspension-cultured tobacco cells in a simple calcium solution. Soil Science and Plant Nutrition, 46(2): 503-514.
  • Jiang HW, Liu MJ, Chen C, Huang CH, Chao LY, Hsieh HL. 2010. A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development. Plant Physiol, 154: 1646-1658.
  • Kariya K, Sameeullah M, Sasaki T, Yamamoto Y. 2017. Overexpression of the sucrose transporter gene NtSUT1 alleviates aluminum-induced inhibition of root elongation in tobacco (Nicotiana tabacum L.). Soil Sci Plant Nutr, 63(1): 45-54.
  • Kopittke PM, Menzies NW, Wang P, Blamey FPC. 2016. Kinetic and nature of aluminium rhizotoxic effects: A review. J Exp Bot, 67: 4451-4467.
  • Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods, 25: 402-408.
  • Mhamdi A, Hager J, Chaouch S, Queval G, Han Y, Taconnat L, Saindrenan P, Gouia H, Issakidis-Bourguet E, Renou JP, Noctor G. 2010. Arabidopsis glutathione reductase1 plays a crucial role in leaf responses to intracellular hydrogen peroxide and in ensuring appropriate gene expression through both salicylic acid and jasmonic acid signaling pathways. Plant Physiol, 153: 1144-1160.
  • Nanda AK, Andrio E, Marino D, Pauly N, Dunand C. 2010. Reactive oxygen species during plant-microorganism early interactions. J Integr Plant Biol, 52: 195-204.
  • Oliveira MB, de Andrade RV, Grossi-de-Sá MF, Petrofeza S. 2015. Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum–Phaseolus vulgaris interaction. Front Microbiol, 6: 1162.
  • Palacıoğlu G, Özer G, Yeken MZ, Ciftci, V, Bayraktar H. 2021. Resistance sources and reactions of common bean (Phaseolus vulgaris L.) cultivars in Turkey to anthracnose disease. Genet Resour Crop Evol, 68(8): 3373-3381.
  • Panda SK, Matsumoto H. 2010. Changes in antioxidant gene expression and induction of oxidative stress in pea (Pisum sativum L.) under Al stress. Biometals, 23: 753-762.
  • Rangel AF, Rao IM, Braun HP, Horst WJ. 2010. Aluminum resistance in common bean (Phaseolus vulgaris) involves induction and maintenance of citrate exudation from root apices. Physiol Plant, 138: 176-190.
  • Rangel AF, Rao IM, Horst WJ. 2007. Spatial aluminium sensitivity of root apices of two common bean (Phaseolus vulgaris L.) genotypes with contrasting aluminium resistance. J Exp Bot, 58: 3895-3904.
  • Şen K, Koca AS, Kaçar G. 2020. Fasulye tohum böceği Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae)’un önemi, biyolojisi, zararı ve mücadelesi. JIST, 10(3): 1518-1527.
  • Tóth B, Moloi MJ, Szőke L, Danter M, Grusak MA. 2021. Cultivar differences in the biochemical and physiological responses of common beans to aluminum stress. Plants, 10(10): 2097.
  • Yamamoto Y, Kobayashi Y, Matsumoto H. 2001. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol, 125(1): 199-208.
  • Yang ZB, Eticha D, Rotter B, Rao IM, Horst WJ. 2011. Physiological and molecular analysis of polyethylene glycol‐induced reduction of aluminium accumulation in the root tips of common bean (Phaseolus vulgaris). New Phytol, 192(1): 99-113.
  • Yeken MZ. 2023. Investigation of genotype × environment interactions for the seed mineral composition in Phaseolus vulgaris L. J Food Compost Anal, 124: 105657.
  • Yeken MZ, Çelik A, Emiralioğlu O, Çiftçi V, Baloch FS, Özer G. 2024. Exploring differentially expressed genes in Phaseolus vulgaris L. during BCMV infection. Physiol Mol Plant Pathol, 130: 102238.

Assessing Aluminum Stress Resilience in Common Bean Roots: Phenotypic, Histochemical, and PvGST/PvPOD Gene Expression Analysis

Year 2024, Volume: 7 Issue: 3, 298 - 304, 15.05.2024
https://doi.org/10.47115/bsagriculture.1450790

Abstract

Common bean (Phaseolus vulgaris L.) is grown in various parts of the world. Aluminum (Al) toxicity poses a significant and widespread challenge in marginal areas with unfavorable soil qualities where common bean is grown. In acidic soils, stable forms of Al dissolve into the soil solution and inhibit root growth and function by injuring the root apex with phytotoxic ions. This leads to the development of a smaller root system, adversely affecting crop yield. In this study, the phenotypic evaluation for relative root elongation of 10 common bean genotypes/cultivars under Al stress (50 µM), the impact of Al toxicity using different histochemical dyes (Evan’s blue and Schiff’s reagent) and the expression levels of PvGST (Glutathione S-transferases) and PvPOD (peroxidase) genes in the root tissues of the most resistant/sensitive common beans under Al stress (50 µM) and control conditions (0 µM) were investigated. The maximum relative root elongation value (71.9%) was found in Önceler-98 cultivar, while the lowest value (14.1%) was obtained from Blksr-19 genotype. Histochemical applications used in the study supported phenotypic results. The cracks at the root tip and high blue color intensity were detected in Schiff’s reagent and Evan’s blue dyes in the Blksr-19, respectively. The expression levels of PvGST and PvPOD genes in the root tissue of the Blksr-19 (Al-sensitive) were highly upregulated at 24 h of Al stress treatment. The results revealed that these genes might be involved in the common bean root tissue's defense mechanism against Al stress for the first time. The findings herein will help plant breeders develop common bean cultivars tolerant to Al toxicity.

References

  • Abdul Kayum M, Nath UK, Park JI, Biswas MK, Choi EK, Song JY, Kim HT, Nou IS. 2018. Genome-wide identification, characterization, and expression profiling of glutathione S-transferase (GST) family in pumpkin reveals likely role in cold-stress tolerance. Genes, 9(2): 84.
  • Ambachew D, Asfaw A, Blair MW. 2023. Phenotypic variability for root traits in andean common beans grown with and without aluminum stress conditions. Agronomy, 13: 619.
  • Ambachew D, Blair MW. 2021. Genome wide association mapping of root traits in the andean gene pool of common bean (Phaseolus vulgaris L.) grown with and without aluminum toxicity. Front Plant Sci, 12: 628687.
  • Baloch FS, Nadeem MA. 2022. Unlocking the genomic regions associated with seed protein contents in Turkish common bean germplasm through genome-wide association study. Turk J Agric For, 46(1): 113-128.
  • Bartoli G, Sanità di Toppi L, Andreucci A, Ruffini Castiglione, M. 2017. Aluminum effects on embryo suspensor polytene chromosomes of Phaseolus coccineus L. Plant Biosys, 152(4): 880-888
  • Butare L, Rao IM, Lepoivre P, Cajiao C, Polania J, Cuasquer J, Beebe S. 2012. Phenotypic evaluation of interspecific recombinant inbred lines (RILs) of Phaseolus species for aluminium resistance and shoot and root growth response to aluminium-toxic acid soil. Euphytica, 186: 715-730.
  • Cançado GMA, DeRosa VE, Fernandez JH, Maron LG, Jorge RA, Menossi M. 2005. Glutathione S-transferase and aluminum toxicity in maize. Funct Plant Biol, 32: 1045-1055.
  • Çelik A, Emiralioğlu O, Yeken MZ, Çiftçi V, Özer G, Kim Y, Baloch FS, Chung YS. 2023. A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection in Phaseolus vulgaris. Front Genet, 14: 1136794.
  • Delhaize E, Ryan PR, Randall P. 1993. Aluminium tolerance in wheat (Triticum aestivum L.) II. Aluminium-stimulated excretion of malic acid from root apices. Plant Physiol, 103: 695-702.
  • Dmitriev AA, Krasnov GS, Rozhmina TA, Kishlyan NV, Zyablitsin AV, Sadritdinova AF, Snezhkina AV, Fedorova MS, Yurkevich OY, Muravenko OV, Bolsheva NL, Kudryavtseva AV, Melnikova NV. 2016. Glutathione S-transferases and UDP-glycosyltransferases are involved in response to aluminum stress in flax. Front Plant Sci, 7: 1920.
  • dos Santos Neto J, Delfini J, Willian ST, Akihide HA, Marcos NJ, Simões Azeredo Gonçalves L, Moda-Cirino V. 2020 Response of common bean cultivars and lines to aluminum toxicity. Agronomy, 10: 296
  • Eticha D, Zahn M, Bremer M, Yang Z, Rangel AF, Rao IM, Horst WJ. 2010. Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaseolus vulgaris) genotypes. Ann Bot, 105(7): 1119-1128.
  • Ezaki B, Suzuki M, Motoda H, Kawamura M, Nakashima S, Matsumoto H. 2004. Mechanism of gene expression of Arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress. Plant Physiol, 134: 1672-1682.
  • FAO. 2023. Food and Agriculture Statistics. Retrieved May 20, 2023 from: https://www.fao.org/faostat/en/#home (accessed date: June 15, 2023).
  • Ikegawa H, Yamamoto Y, Matsumoto H. 2000. Responses to aluminium of suspension-cultured tobacco cells in a simple calcium solution. Soil Science and Plant Nutrition, 46(2): 503-514.
  • Jiang HW, Liu MJ, Chen C, Huang CH, Chao LY, Hsieh HL. 2010. A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development. Plant Physiol, 154: 1646-1658.
  • Kariya K, Sameeullah M, Sasaki T, Yamamoto Y. 2017. Overexpression of the sucrose transporter gene NtSUT1 alleviates aluminum-induced inhibition of root elongation in tobacco (Nicotiana tabacum L.). Soil Sci Plant Nutr, 63(1): 45-54.
  • Kopittke PM, Menzies NW, Wang P, Blamey FPC. 2016. Kinetic and nature of aluminium rhizotoxic effects: A review. J Exp Bot, 67: 4451-4467.
  • Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods, 25: 402-408.
  • Mhamdi A, Hager J, Chaouch S, Queval G, Han Y, Taconnat L, Saindrenan P, Gouia H, Issakidis-Bourguet E, Renou JP, Noctor G. 2010. Arabidopsis glutathione reductase1 plays a crucial role in leaf responses to intracellular hydrogen peroxide and in ensuring appropriate gene expression through both salicylic acid and jasmonic acid signaling pathways. Plant Physiol, 153: 1144-1160.
  • Nanda AK, Andrio E, Marino D, Pauly N, Dunand C. 2010. Reactive oxygen species during plant-microorganism early interactions. J Integr Plant Biol, 52: 195-204.
  • Oliveira MB, de Andrade RV, Grossi-de-Sá MF, Petrofeza S. 2015. Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum–Phaseolus vulgaris interaction. Front Microbiol, 6: 1162.
  • Palacıoğlu G, Özer G, Yeken MZ, Ciftci, V, Bayraktar H. 2021. Resistance sources and reactions of common bean (Phaseolus vulgaris L.) cultivars in Turkey to anthracnose disease. Genet Resour Crop Evol, 68(8): 3373-3381.
  • Panda SK, Matsumoto H. 2010. Changes in antioxidant gene expression and induction of oxidative stress in pea (Pisum sativum L.) under Al stress. Biometals, 23: 753-762.
  • Rangel AF, Rao IM, Braun HP, Horst WJ. 2010. Aluminum resistance in common bean (Phaseolus vulgaris) involves induction and maintenance of citrate exudation from root apices. Physiol Plant, 138: 176-190.
  • Rangel AF, Rao IM, Horst WJ. 2007. Spatial aluminium sensitivity of root apices of two common bean (Phaseolus vulgaris L.) genotypes with contrasting aluminium resistance. J Exp Bot, 58: 3895-3904.
  • Şen K, Koca AS, Kaçar G. 2020. Fasulye tohum böceği Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae)’un önemi, biyolojisi, zararı ve mücadelesi. JIST, 10(3): 1518-1527.
  • Tóth B, Moloi MJ, Szőke L, Danter M, Grusak MA. 2021. Cultivar differences in the biochemical and physiological responses of common beans to aluminum stress. Plants, 10(10): 2097.
  • Yamamoto Y, Kobayashi Y, Matsumoto H. 2001. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol, 125(1): 199-208.
  • Yang ZB, Eticha D, Rotter B, Rao IM, Horst WJ. 2011. Physiological and molecular analysis of polyethylene glycol‐induced reduction of aluminium accumulation in the root tips of common bean (Phaseolus vulgaris). New Phytol, 192(1): 99-113.
  • Yeken MZ. 2023. Investigation of genotype × environment interactions for the seed mineral composition in Phaseolus vulgaris L. J Food Compost Anal, 124: 105657.
  • Yeken MZ, Çelik A, Emiralioğlu O, Çiftçi V, Baloch FS, Özer G. 2024. Exploring differentially expressed genes in Phaseolus vulgaris L. during BCMV infection. Physiol Mol Plant Pathol, 130: 102238.
There are 32 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering (Other)
Journal Section Research Articles
Authors

Mehmet Zahit Yeken 0000-0003-0490-371X

Publication Date May 15, 2024
Submission Date March 11, 2024
Acceptance Date April 19, 2024
Published in Issue Year 2024 Volume: 7 Issue: 3

Cite

APA Yeken, M. Z. (2024). Assessing Aluminum Stress Resilience in Common Bean Roots: Phenotypic, Histochemical, and PvGST/PvPOD Gene Expression Analysis. Black Sea Journal of Agriculture, 7(3), 298-304. https://doi.org/10.47115/bsagriculture.1450790

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