Sweet chestnut, Castanea sativa Mill., is a significant species in Türkiye. The tree has been encountered with numerous diseases. Chestnut blight, caused by Cryphonectria parasitica (Murrill) M. E. Barr, is one of the most common diseases having spread to every chestnut-growing region. In this study, the effects of Bellis® (25.2% Boscalid + 12.8% Pyraclotrobin), a fungicide commonly used in plant disease control, were evaluated at different doses (10 ppm and 50 ppm) against the chestnut blight agent C. parasitica, which had not been previously studied for its effects in vitro. The isolates included two virulent strains (Cp1_2023, Cp2_2023) and two hypovirulent strains (Cp7_2023, Cp9_2023). The effects of Boscalid + Pyraclotrobin on the mycelial growth of the pathogenic fungus were determined for the first time in vitro, and these effects were detected at the sdhA and sdhB genes expression level. The results showed that the mycelial growth and pathogenicity of C. parasitica isolates in the fungicide-treated medium were consistent with the gene expression profile. Specifically, the virulent Cp1_2023 isolate exhibited higher mycelial growth, pathogenicity, and gene expression levels compared to the other isolate. In conclusion, it is recommended to conduct more detailed studies before using fungicides and to employ them in controlled environments.
Ethics committee approval was not required for this study because there was no study on animals or humans.
We would like to thank the General Directory of Forestry and the Regional Directory of Forestry for providing the study materials.
Primary Language | English |
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Subjects | Gene Expression |
Journal Section | Research Articles |
Authors | |
Publication Date | May 15, 2024 |
Submission Date | March 15, 2024 |
Acceptance Date | April 19, 2024 |
Published in Issue | Year 2024 Volume: 7 Issue: 3 |