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The microbiota of long-living and cancer-free blind mole rat $\textit{(Nannospalax xanthodon)}$ from the edge of its distribution in Northern Anatolia

Year 2023, Volume: 32 Issue: 2, 105 - 118, 30.12.2023
https://doi.org/10.53447/communc.1281221

Abstract

The mammalian gut is colonized by microorganisms that affect development, immune system, energy metabolism, and reproduction. The majority of studies focused on laboratory or domestic animals in artificial setups, leaving the research focused on wild species underrepresented. The Anatolian Blind Molerat (hereafter ABMR), $\textit{Nannospalax xanthodon}$, is a subterranean rodent that receives much attention due to its unique traits, such as tolerance to extreme hypoxic stress, resistance to cancer, and longer lifespan compared to similarly sized rodents. In this study, we characterize the gut microbiota of ABMR from its northernmost geographic distribution using 16S rRNA metabarcoding and compare our results with the microbiome characteristics of a few other ABMR populations studied previously, as well as other rodent species. The 16S rRNA barcode dataset revealed that approximately 90% of the ABMR gut microbiota comprises Firmicutes and Bacteriodota bacterial phyla, typical of most mammals. In addition, the ABMR gut microbiota has a high abundance of performance- and longevity-linked bacterial families. Overall, our results generally align well with the previous studies on blind molerats and emphasize the importance of studying the microbiome of natural populations.

Thanks

This study was supported by TÜBİTAK grant 220Z032, and the collection of samples was funded by TUBITAK 119Z400. DC and JK were supported by Czech Science Foundation (19-19307S). The numerical calculations reported in this paper were partially performed at TÜBİTAK ULAKBİM, High Performance and Grid Computing Center (TRUBA resources).

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Year 2023, Volume: 32 Issue: 2, 105 - 118, 30.12.2023
https://doi.org/10.53447/communc.1281221

Abstract

References

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  • van Leeuwen, P., Mykytczuk, N., Mastromonaco, G.F., Schulte-Hostedde, A.I., Effects of captivity, diet, and relocation on the gut bacterial communities of white-footed mice. Ecology and Evolution, 10 (11) (2020), 4677–4690. https://doi.org/10.1002/ece3.6221.
  • Belheouane, M., Vallier, M., Čepić, A., Chung, C.J., Ibrahim, S., Baines, J.F., Assessing similarities and disparities in the skin microbiota between wild and laboratory populations of house mice. The ISME Journal, 14 (10) (2020), 2367–2380. https://doi.org/10.1038/s41396-020-0690-7.
  • Wang, J., Linnenbrink, M., Künzel, S., Fernandes, R., Nadeau, M.-J., Rosenstiel, P., Baines, J.F., Dietary history contributes to enterotype-like clustering and functional metagenomic content in the intestinal microbiome of wild mice. Proceedings of the National Academy of Sciences of the United States of America, 111 (26) (2014), E2703-10. https://doi.org/10.1073/pnas.1402342111.
  • Kreisinger, J., Cížková, D., Vohánka, J., Piálek, J., Gastrointestinal microbiota of wild and inbred individuals of two house mouse subspecies assessed using high-throughput parallel pyrosequencing. Molecular Ecology, 23 (20) (2014), 5048–5060. https://doi.org/10.1111/mec.12909.
  • Weldon, L., Abolins, S., Lenzi, L., Bourne, C., Riley, E.M., Viney, M., The gut microbiota of wild mice. Plos One, 10 (8) (2015), e0134643. https://doi.org/10.1371/journal.pone.0134643.
  • Debebe, T., Biagi, E., Soverini, M., Holtze, S., Hildebrandt, T.B., Birkemeyer, C., Wyohannis, D., Lemma, A., Brigidi, P., Savkovic, V., König, B., Candela, M., Birkenmeier, G., Unraveling the gut microbiome of the long-lived naked mole-rat. Scientific Reports, 7 (1) (2017), 9590. https://doi.org/10.1038/s41598-017-10287-0.
  • Ley, R.E., Hamady, M., Lozupone, C., Turnbaugh, P.J., Ramey, R.R., Bircher, J.S., Schlegel, M.L., Tucker, T.A., Schrenzel, M.D., Knight, R., Gordon, J.I., Evolution of mammals and their gut microbes. Science, 320 (5883) (2008), 1647–1651. https://doi.org/10.1126/science.1155725.
  • Zhou, T., Liu, S., Jiang, A., Comparison of gut microbiota between immigrant and native populations of the Silver-eared Mesia (Leiothrix argentauris) living in mining area. Frontiers in Microbiology, 14 (2023), 1076523. https://doi.org/10.3389/fmicb.2023.1076523.
  • Linnenbrink, M., Wang, J., Hardouin, E.A., Künzel, S., Metzler, D., Baines, J.F., The role of biogeography in shaping diversity of the intestinal microbiota in house mice. Molecular Ecology, 22 (7) (2013), 1904–1916. https://doi.org/10.1111/mec.12206.
  • Arslan, A., Kryštufek, B., Matur, F., Zima, J., Review of chromosome races in blind molerats (Spalax and Nannospalax). Folia Zoologica, 65 (4) (2016), 249–301. https://doi.org/10.25225/fozo.v65.i4.a1.2016.
  • Shams, I., Avivi, A., Nevo, E., Oxygen and carbon dioxide fluctuations in burrows of subterranean blind molerats indicate tolerance to hypoxic-hypercapnic stresses. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 142 (3) (2005), 376–382. https://doi.org/10.1016/j.cbpa.2005.09.003.
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Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Halil Mert Solak 0000-0001-6877-9274

Efe Sezgin 0000-0002-8000-7485

Dagmar Cizkova 0000-0001-5031-7792

Jakub Kreisinger 0000-0001-9375-9814

Faruk Çolak 0000-0003-3985-7864

Ortaç Çetintaş 0000-0001-7601-2540

Alexey Yanchukov 0000-0002-9613-8770

Early Pub Date November 21, 2023
Publication Date December 30, 2023
Acceptance Date June 24, 2023
Published in Issue Year 2023 Volume: 32 Issue: 2

Cite

Communications Faculty of Sciences University of Ankara Series C-Biology.

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