Investigation of in vitro antidiabetic and antioxidant activity of hawthorn vinegar obtained from Endemic Crataegus tanacetifolia (Poir.) Pers.

Year 2023, Volume: 6 Issue: 2, 104 - 108, 30.12.2023

Feyza Akgün Nigar Sıla Tuğlu Yasemin Gülbahar Açıl Nuraniye Eruygur

https://doi.org/10.46239/ejbcs.1228402

Abstract

In this study, the in vitro antidiabetic, antioxidant activity and total flavonoid content (TFC) and total phenolic content (TPC) of vinegar obtained from endemic Crataegus tanacetifolia (Lam.) Pers. (Rosaceae), (hawthorn) were examined. The hawthorn vinegar obtained from Malatya province (MS) and the vinegar (TS) obtained from Konya were used as study material. Their antidiabetic activity was determined by α-amylase and α-glucosidase inhibitory methods. Antioxidant activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferrous iron chelating (FCA) assays. The absorbance were read in the Elisa reader and evaluated with Excel and GraphPad programs. The MS has been found to have higher α- amylase (95.12± 3.71%) and α-glucosidase inhibitory (81.62 ± 0.33%) effects. The TS demonstrated (94.13 ± 3.85%) α-amylase and (75.35 ± 2.19%) α-glucosidase inhibitory activity, respectively. The TPC was found to be in TS (467.59 ± 6.73) mg GAE/mL MS (328.46 ± 5.50) mg GAE/mL. The TFC was found as (1.94 ± 10.36) mg CE/mL and (1.32 ± 10.96) mg CE/mL in TS and MS vinegar, respectively. The FCA was found to be in TS (33.37 ± 0.53%) MS (31.08 ± 10.87%). The DPPH radical scavenging activity was found as (73.82 ± 2.12%) in TS and (80.12 ± 4.45%) in MS. ABTS radical scavenging activity was found to be the highest in TS with (82.51± 0.78%) and in MS found as (78.65 ± 0.55%). The antidiabetic, antioxidant activity, TPC and TFC determinations of these vinegars were performed for the first time with these methods.

Keywords

Bioactivity, Crataegus tanacetifolia, Phytochemical, Vinegar

Supporting Institution

TUBITAK 2209/A Undergraduate Student Project

Project Number

1919B012102103

Thanks

We would like to thank the TUBITAK 2209/A Undergraduate Student Project for the financial support (NO: 1919B012102103).

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