MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON

Year 2018, Volume: 4 Issue: 1, 12 - 19, 01.01.2018

Burak Evren İnanan Fevzi Yılmaz

Abstract

Boron (B) is required for organisms as an essential
nutrient, whereas could be toxic in definite doses. In this study, for the
first time, the effects of water-borne and food-borne B on fish sperm motility
were initially determined and these sperm samples were cryopreserved in liquid
nitrogen aiming to reveal how the quality of these samples are affected by
cryopreservation. 12 mature male goldfish (Carassius
auratus) individuals for each treatment performed in triplicate for 45 days
were used in the study. Fish were fed with diets containing 1 mg, 5 mg and 10
mg of B per kg feed for foodborne experiments. In waterborne experiments, fish
were kept in aquariums containing 1 mg/L, 10 mg/L and 20 mg/L of B. In both
fresh and cryopreserved samples, the motility durations (s) and percentages of
the sperm samples, vitality of spermatozoa (%) were determined. In the fresh
samples, percentages of sperm motility and viability in the both B treatments
decreased compared to the control, while increasing B concentration. After
cryopreservation, the sperm samples in 5 mg of B per kg feed treatment has
shown higher duration of sperm motility. Therefore, it is shown that feed-borne
B could improve sperm resistance to cold shock.

Keywords

Boron, Carassius auratus, Sperm motility, Sperm vitality, Cryopreservation

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