Kırmızı Alglerden Gracilaria verrucosa'nın Biyomas Verimi ve Kimyasal Kompozisyonu Üzerine Fotoperiyot Uygulamalarının Etkisinin Belirlenmesi

Year 2023, Volume: 8 Issue: 4, 756 - 763, 31.12.2023

İlknur Ak Melis Yılmaz Gülen Türker

https://doi.org/10.35229/jaes.1226819

Abstract

Bu çalışmada kırmızı alglerden Gracilaria verrucosa (Hudson) Papenfuss tank kültür sistemlerinde 100 µmol foton m-2 s -1 ışık şiddetinde, farklı foto periyotlar (24:00, 16:08, 12:12 ve 08:16 (A:K)) uygulanarak algin büyüme hızında ve kimyasal içeriğinde meydana gelen değişimler izlenmiştir. Çalışma sonucunda deneme grupları arasında en yüksek büyüme hızı 16:8 (A:K) foto periyot uygulanan grupta bulunmuştur. Deneme gruplarının klorofil a içerikleri aydınlanma süresi arttıkça azalmıştır. Büyüme hızı arttıkça algin fikosiyanin ve fikoeritrin içeriklerinin azaldığı belirlenmiştir. Çalışmada grupların ham protein içerikleri %9,14±0,13 (24:24) ile %10,92±0,25 (12:12) arasında değişim göstermiştir. Tüm deneme gruplarının yağ içerikleri %1’den az bulunmuştur. Alg talluslarının kül içerikleri arasında istatistiksel olarak önemli derece farklılıkların olmadığı görülmüştür (p>0,05). Deneme gruplarının agar içerikleri %8,36±0,24– 13,19±1,09 arasında değişim göstermiştir. En yüksek agar içeriği 8:16 foto periyot uygulanan grupta saptanmıştır. Yüksek ışık şiddeti ve uzun aydınlanma sürelerinin algin serbest radikal temizleme aktivitesini arttırdığı çalışma sonucunda saptanmıştır. Deneme gruplarının toplam fenolik madde içerikleri ile 1,82±0,03 (8:16) ile 2,84±0,04 (24:24) mg GAE g−1 ekstrakt arasında değişim göstermiştir. En yüksek toplam flavonoid içeriği 8:16 (A:K) uygulanan grupta saptanmıştır.

Keywords

agar, antioksidan, büyüme hızı, Gracilaria verrucosa, pigment

Supporting Institution

Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon birimi

Project Number

Fhd-2021-3677

Thanks

Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon birimi tarafınfan Fhd-2021-3677 proje numaralı ve “Kırmızı Alglerden Gracilaria verrucosa’nın Biyomas Verimi ve Kimyasal Kompozisyonu Üzerine Işık Şiddeti ve Fotoperiyot Uygulamalarının Etkisinin Belirlenmesi” başlıklı proje ile desteklenmiştir

Effect of Photoperiod Applications on Biomass Yield and Chemical Composition of Red Algae Gracilaria verrucosa

Abstract

In this study, the changes in the growth rate and chemical content of algal extracts from Gracilaria verrucosa (Hudson) were examined under Papenfuss tank culture systems with 100 µmol photon m-2 s -1 light intensity and different photo periods (24:00, 16:08, 12:12 and 08:16 (L:D)). As a result of the study, the highest growth rate among the experimental groups was found in the 16:8 (L:D) photoperiod group. Chlorophyll a content of the groups decreased as the illumination time increased while phycocyanin, phycoerythrin contents decreased as the growth rate increased. The crude protein content of the groups varied between 9.14±0.13 (24:24) and 10.92±0.25 (12:12). Fat content of all groups was found to less than 1%. There was no statistically significant difference in ash content of algae thallus (p>0.05). The agar contents of the experimental groups varied between %8.36±0.24-13.19±1.09. The highest agar content was found in the group treated with the 8:16 photo period. As a result of the study, it was determined that high light intensity and long illumination durations increase the free radical scavenging activity of algae. The total phenolic content of the experimental groups varied between 1.82±0.03 (8:16) and 2.84±0.04 (24:24) mg GAE g−1 extract. The highest total flavonoid content was found in the 8:16 (L:D) group.

Keywords

agar, antioxidant, growth rate, Gracilaria verrucosa, pigment

Project Number

Fhd-2021-3677

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