Antibacterial, Antioxidant, Antidiabetic Potentials and Chemical Composition of Nicotiana glauca Graham Leaf Extract

Year 2023, Volume: 8 Issue: 4, 700 - 706, 31.12.2023

Melis Sümengen Özdenefe Aysun Mercimek Takcı Fikret Büyükkaya Kayış

https://doi.org/10.35229/jaes.1325678

Abstract

The purpose of this study is to investigate the chemical composition and potentiality of Nicotiana glauca G. leaf extract taken from the Turkish Republic of Northern Cyprus (TRNC), such as antibacterial, antioxidant, and antidiabetic compounds. In February 2020, a leaf of N. glauca was gathered in the Kyrenia district of Northern Cyprus. Methanol was used to extract the leaf sample. The content of essential oils in the extract was determined using gas chromatography and a headspace system test. Several methods were used to investigate the extract's antibacterial, antioxidant activities, and antidiabetic potential. On all microorganisms examined, the leaf methanol extract of N. glauca had no antibacterial activity. Total phenolic and flavonoid content, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, metal chelating, and phosphomolybdenum assays were all active with the methanol extract. The extract showed no antidiabetic efficacy. Furthermore, the essential oil was not found in the extract's chemical composition. According to the findings, the presence of antioxidant components in N. glauca leaf extract can protect against the detrimental effects of free radicals. Its application in Northern Cyprus becomes even more crucial as a result of this potential feature.

Keywords

Bioactive compounds, doctor tree, methanol extract, Northern Cyprus

Nicotiana glauca Graham Yaprak Ekstraktının Antibakteriyel, Antioksidan, Antidiyabetik Potansiyelleri ve Kimyasal Bileşimi

Abstract

Bu çalışmanın amacı, Kuzey Kıbrıs Türk Cumhuriyeti'nden (KKTC) alınan Nicotiana glauca G. yaprak ekstraktının kimyasal bileşimini ve antibakteriyel, antioksidan ve antidiyabetik bileşikler gibi potansiyelini araştırmaktır. Şubat 2020'de Kuzey Kıbrıs'ın Girne ilçesinde N. glauca yaprağı toplandı. Yaprak örneğini ekstrakte etmek için metanol kullanıldı. Ekstrakttaki uçucu yağların içeriği, gaz kromatografisi ve headspace sistem testi kullanılarak belirlendi. Ekstraktın antibakteriyel, antioksidan aktivitelerini ve antidiyabetik potansiyelini araştırmak için çeşitli yöntemler kullanıldı. İncelenen tüm mikroorganizmalar üzerinde, N. glauca'nın yaprak metanol ekstraktının antibakteriyel aktivitesi yoktu. Toplam fenolik ve flavonoid içeriği, 1,1-difenil-2-pikrilhidrazil radikal süpürme aktivitesi, ferrik indirgeyici antioksidan gücü, metal şelatlama ve fosfomolibden deneylerinin tümü metanol ekstraktı ile aktifti. Ekstrakt antidiyabetik etkinlik göstermedi. Ayrıca, ekstraktın kimyasal bileşiminde uçucu yağ bulunmadı. Elde edilen bulgulara göre, N. glauca yaprak ekstraktında antioksidan bileşenlerin bulunması, serbest radikallerin zararlı etkilerine karşı koruma sağlayabiliyor. Bu potansiyel özelliği nedeniyle Kuzey Kıbrıs'ta uygulanması daha da önem kazanmaktadır.

Keywords

Biyoaktif bileşikler, doktor ağacı, metanol ekstrakt, Kuzey Kıbrıs

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