Accurate and Sensitive Determination of Mefenpyr-diethyl in Barley, Oat and Corn Silk Matrices by Gas Chromatography – Flame Ionization Detector (GC–FID)

Year 2024, Volume: 14 Issue: 1, 23 - 27, 29.04.2024

Hakan Serbest

Abstract

Pesticides are frequently utilized in the cultivation of agricultural products for human consumption to prevent / minimize the detrimental effects caused by pests and to keep the yield at the desired levels at harvest time. Besides the benefits of pesticides, unconscious use of pesticides causes the occurrence of different diseases. Monitoring the levels of pesticide residues is of vital importance for the environment, human, and other living organisms. In the present study, an analytical method was reported for the determination of mefenpyr-diethyl (MFD) as a member of the herbicide group by gas chromatography flame ionization detector (GC-FID) system with high accuracy and sensitivity. An in-column temperature program was established to effectively separate the analyte, and MFD was determined at a retention time of 5.2 min. The limit of detection (LOD), the limit of quantitation (LOQ), and the linear working range were found to be 0.01 mg/L, 0.04 mg/L, and 0.07-29.7 mg/L, respectively. The applicability of the determination method was investigated by recovery studies with barley, oat, and corn silk matrices. No analytical signal was recorded for MFD in blank samples of all three species. Recovery results close to 100% showed that MFD could be determined with high accuracy in barley, oat, and corn silk matrices.

Keywords

GC-FID, mefenpyr-diethyl, herbicide, grain matrix

Ethical Statement

No ethical approval is required.

Supporting Institution

None

Thanks

None

Mefenpir-dietilin Arpa, Yulaf ve Mısır Püskülü Matrikslerinde Gaz Kromatografisi - Alev İyonizasyon Dedektörü (GC-FID) ile Doğru ve Hassas Tayini

Abstract

İnsan tüketiminde olan zirai ürünlerin yetiştirilmesinde zararlıların neden olacağı bozucu etkileri önlemek / en aza indirmek ve hasat sırasında verimin istenen seviyelerde tutmak amacıyla pestisitlerden sıklıkla yararlanılmaktadır. Pestisitlerin faydalarının yanı sıra, bilinçsiz kullanımı farklı hastalıkların ortaya çıkmasına neden olmaktadır. Pestisit kalıntılarının seviyelerinin izlenmesi çevre, insanlar ve diğer canlı organizmalar için hayati önem taşımaktadır. Bu çalışmada, herbisit grubunun bir üyesi olan mefenpir-dietilin (MFD) gaz kromatografisi alev iyonizasyon dedektörü (GC-FID) sistemi ile yüksek doğruluk ve hassasiyetle tayini için analitik bir yöntem rapor edilmiştir. Analitin etkili bir şekilde ayrılması için kolon içi sıcaklık programı oluşturulmuş ve 5,2 dakikalık bir alıkonma süresinde MFD tayin edilmiştir. Gözlenebilme limiti (GL), tayin limiti (TL) ve doğrusal çalışma aralığı sırasıyla 0,01 mg/L, 0,04 mg/L, ve 0,07-29,7 mg/L olarak bulunmuştur. Tayin yönteminin uygulanabilirliği arpa, yulaf ve mısır püskülü örnekleri ile yapılan geri kazanım çalışmaları ile araştırılmıştır. Her üç türün kör numunelerinde MFD için analitik bir sinyal kaydedilmemiştir. %100'e yakın geri kazanım sonuçları arpa, yulaf ve mısır püskülü örneklerinde yüksek doğrulukta MFD’nin tayin edilebileceğini göstermiştir.

Keywords

GC-FID, mefenpir-dietil, herbisit, tahıl matriksi

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