Bacterial agent isolation from calves with arthritis and antibiotic susceptibility of isolates

Year 2023, Volume: 8 Issue: 3, 161 - 171, 31.12.2023

Elif Çelik Celal Şahin Ermutlu Aliye Gülmez Sağlam Özgür Çelebi Özgür Aksoy Mustafa Reha Coşkun İsa Özaydın

Abstract

Septic arthritis is frequently encountered in neonatal calves, and it causes significant economic losses as a result of high mortality rates. The main objective of this study was to identify the etiology of septic arthritis by determinnig the antibiotic susceptibility of the isolated species and to isolate and molecularly identify the bacterial agents that cause arthritis in calves, and to contribute to the selection of appropriate antibiotics in the treatment of the disease caused by these agents.This study bacteriologically examined synovial fluid samples taken from 65 calves 0–3 months old that were brought to the animal hospital clinics of the Faculty of Veterinary Medicine of Kafkas University between 2018 and 2020. Conventional techniques were applied to isolate and identify the agents. While the agent was isolated from 5 samples, it could not be isolated from 60 samples. Erysipelothrix spp., Staphylococcus aureus, and Mycoplasma bovis were each isolated in a single sample, Corynebacterium pyogenes was isolated in two samples, and C. pyogenes and Escherichia coli were detected together in one sample. Results of direct PCR from the synovial fluid samples found C. pyogenes DNA in 18 samples, S. aureus DNA in 8 samples, and C. pyogenes and S. aureus DNA together in 3 samples. The results of the antibiogram test found that E. coli was resistant to amoxicillin-clavulanic acid, gentamicin, ampicillin-sulbactam, ciprofloxacin, and trimethoprim sulfamethoxazole; S. aureus was resistant to penicillin G, oxytetracycline, erythromycin, cloxacillin, chlorampheniol, neomycin, and lincomycin; and C. pyogenes was resistant to neomycin, gentamicin, erythromycin, oxytetracycline, and chloramphenicol. The isolates were sensitive to streptomycin, ofloxacin, danofloxacin, ampicillin, cefoperazone, and vancomycin. Erisipelothrix spp. were sensitive to erythromycin, ciprofloxacin, ampicillin, oxytetracycline, ampicillin-sulbactam, and chloramphenicol antibiotics, and resistant to vancomycin, trimethoprim-sulfamethoxazole, penicillin G, amoxicillin-clavulanic acid, gentamicin, neomycin, and lincomycin.

Keywords

Calf, arthritis, C. pyogenes, Erysipelothrix spp., M. bovis, S. aureus, PCR, antibiogram

Project Number

2018-TS-01

References

• Arican, M., Elma, E., & Ozkan, K. (1998). Clinical evaluations of calves with infectious arthritis. Veteriner Cerrahi Dergisi, 4(1-2), 5-7.
• Arican, M., Erol, H., Altan, S., Koylu, O. (2022). The use of infrared thermogrophy ın the early diagnosis of septic arthritis in calves. Bulgarian Journal of Veterinary Medicine, 25(2), 298-307. DOI: 10.15547/bjvm.2020-0035.
• Ayhanci, T., Terzi, H.A., Köroğlu, M., Erkin, A., & Altındis, M. (2018). A rare case of osteomyelitis caused by Erysipelothrix rhusiopathiae. Online Türk Sağlık Bilimleri Dergisi, 3(4), 258-262. https://doi.org/10.26453/otjhs.433710.
• Bailey, J.V. (1985). Bovine arthritidies classification, diagnosis, prognosis, and treatment. Veterinary clinics of North America,: Food Animal Practice, 1(1), 39-51. https://doi.org/10.1016/S0749-0720(15)31349-9.
• Balboni, V.G., Shapiro, I. & Kydd, D.M. (1945). The Penetration of penicillin in to the joint fluid following intramuscular administration. American Journal of Medical Sciences, 210 : 588-591.
• Balootaki, P.A., Amin, M., Haghparasti, F., & Rokhbakhsh-Zamin, F. (2017). Isolation and detection of Erysipelothrix rhusiopathiae and its distribution in humans and animals by phenotypical and molecular methods in Ahvaz-Iran in 2015. Iranian Journal of Medical Sciences, 42(4), 377-383. PMID: 28761204.
• Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45, 493-496. PMID: 5325707.
• Beccati, F., Gialletti, R., Passamonti, F., Nannarone, S., Di Meo, A. & Pepe, M. (2015). Ultrasonographic findings in 38 horses with septic arthritis/ tenosynovitis. Veterinary Radiology & Ultrasound, 56:68-76.
• Bumin, A., Temizsoylu, M.D., Kibar, M., & Alkan, Z. (2001). Clinical, radiographic and arthroscopic evaluation of purulent arthritis in calves. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 48(3), 183-187. https://doi.org/10.1501/Vetfak_0000001615.
• Carter, G.K. (1991). Inflammatory, infectious and immune diseases. In P. T. Colahan, I. G. Mayhew, A. M. Merritt, & J. N. Moore (Eds.), Equine Medicine and Surgery, (4th ed., pp. 1281–1293). Am Vet Public, Goleta, CA.
• Cihan, M., Ozaydin, I., Baran, V., & Kılıç, E. (2002). Treatment of acute arthritis with inraarticular administariton of dimethylsulfoxide (DMSO) in calves. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 8 (1), 11-15.
• Clinical and Laboratory Standards Institute (CLSI). (2020). Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI supplement M100. Wayne, PA, Clinical and Laboratory Standards Institute.
• Cakır, S., Canpolat, I., & Yerlikaya, Z. (2019). The treatment of septic arthritis in the calves with arthroscopic lavage. International VETEXPO, Veterinary Sciences Congress September 20-22, Istanbul, Turkey.
• Dakhael, K.H., Rahim, R.A., Neela, V.K., Al-Obaidi, J.R., Hun, T.G., & Yusoff, K. (2016). Methicillin-resistant Staphylococcus aureus biofilms and their influence on bacterial adhesion and cohesion. BioMed Research International, 1-14. https://doi.org/10.1155/2016/4708425.
• Desrochers, A. (2004a). Septic arthritis. In S. L. Fubini, & N. G. Ducharme (Eds), Farm Animal Surgery (pp. 494–497). Saunders.
• Desrochers, A. (2004b). Septic Arthritis. In S. L. Fubini, & N. G. Ducharme (Eds), Farm Animal Surgery (pp. 330–336). Elseiver, St Louis, MI.
• Dogan, E., Yanmaz, L.E., Okumus, Z., Kaya, M., Senocak, M.G., & Cengiz, S. (2016). Radiographic, ultrasonographic and thermographic findings in neonatal calves with septic arthritis: 82 cases (2006-2013). Atatürk Universtiy Journal of Veterinary Sciences, 11, 6-12. https://doi.org/10.17094/avbd.51116.
• Francoz, D., Desrochers, A., Fecteau, G., Desautels, C., Latouche, S.C., & Fortin, M. (2005). Synovial fluid changes in induced infectious arthritis in calves. Journal of Veterinary Internal Medicine, 19, 336-343. https://doi.org/10.1892/0891-6640(2005)19[336:sfciii]2.0.co;2.
• Giguère S., Prescott J.F., Baggot J.D., Walker R.D. & Dowling P.M. 2010. Terapia antimicrobiana em medicina veterinária. 4ª ed. Roca, São Paulo. 683p.
• Gharagozlou, M., Najafi, J., Tabatabayi, A.H., & Khazrainia, P. (2004). A pathologic and microbiologic study on bovine arthritis associated with Mycoplasma spp. Archives Razi Institute, 58, 97-104. https://doi.org/10.22092/ARI.2004.103829.
• Goodarzi, M., Khamesipour, F., Mahallati, S.A., Dehkordi, M.K., & Azizi, S. (2015). Study on prevalence of bacterial causes in calves arthritis. Journal of Agricultural and Biological Science, 10, 206-212.
• Gokhan, N., & Ozturk, S. (2016). Evaluation of the clinical, radiological, ultrasonographical and histopathological findings of arthritis in calves. Erciyes University Journal of Faculty of Veterinary Medicine, 13(1), 19-29.
• Haerdi-Landerer, M.C., Habermacher, J., Wenger, B., Suter, M.M. & Steiner, A. (2010). Slow release antibiotics for treatment of septic arthritis in large animals. Veterinary Journal, 184:14-20.
• Hall, M.S., Pollock, P.J. & Russell, T. (2012). Surgical treatment of septic physitis in 17 foals. Australian Veterinary Journal. 90:479-484.
• Hananeh, W.M., Al Momani, W.M., Ababneh, M.M., & Abutarbush, S.M. (2018). Mycoplasma bovis arthritis and pneumonia in calves in Jordan: An emerging disease. Veterinary World, 1(12), 1663-1666. https://doi.org/10.14202/vetworld.2018.1663-1668.
• Herrell, W.E., Nichols, D.R. & Heilman, D.H. (1944). Penicillin. Its usefulness, diffusion and detection with analysis of 150 cases in which it was employed. Journal of the American Medical Associatio. 125 15: 1003-1011.
• Ismail, Z.B., Al-Rukibat, R., Al-Tarazi, Y., & Al-Zghoul, M.B. (2007). Synovial fluid analysis and bacterial findings in arthritic joints of juvenile male camel (Camelus dromedarius) calves. Journal of Veterinary Medicine, 54, 66-69. https://doi.org/10.1111/j.1439-0442.2007.00889.x.
• Jesse, F.F.A., Bitrus, A.A., Abba, Y., Mahadzar, M., Hambali, I.U., Peter, I.D., Haron, A.W., Lila, M.A.M., Saharee, A.Z. (2017). Clinical management of septic arthritis in a sheep: A case report. Advances in Animal and Veterinary Sciences, 5(6), 267-270. Doı: 10.17582/journal.aavs/2017/5.6.267.270
• Jost, A., & Sickinger, M. (2021). Helcococcus ovis associated with septic arthritis and bursitis in calves–a case report. BMC Veterinary Research, 17:291. https://doi.org/10.1186/s12917-021-02996-6.
• Kallo, O.J., Abdul-Ghani, Z.G. & Khalid, F.R. (1997). Incidence of joint infection in calves. The Bovine Practitioner, 31, 2.
• Madison, J.B., Sommer, M., & Spencer, P.A. (1991). Relations among synovial membrane histopathologic findings, synovial fluid cytologic findings, and bacterial culture results in horses with suspected infectious arthritis: 64 cases (1979-1987). Journal of the American Veterinary Medical Association, 198, 1655-1661. PMID: 2061187
• Marmur, J. (1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology, 3, 208-218.
• Martineau, F., Picard, F.J., Roy, P.H., Quellette, M., & Bergeron, M.G. (1998). Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. Journal of Clinical Microbiology, 36(3), 618-623. https://doi.org/10.1128/JCM.36.3.618-623.1998.
• Maunsell, F.P., Woolums, A.R., Francoz, D., Rosenbusch, R.F., Step, D.L., Wilson, D.J., & Janzen, E.D. (2011). Mycoplasma bovis infections in cattle. Journal of Veterinary Internal Medicine, 25, 772-783. https://doi.org/10.1111/j.1939-1676.2011.0750.x.
• Morton, A.J. (2005). Diagnosis and treatment of septic arthritis. Vet. Clin. North Am., Equine Pract. 21:627-649.
• Motta, R. G., Martins, L. S.A., Motta, I. G., Guerra, S. T., de Paula, C.L., Bolanos, C.A.D., da Silva,R.C., & Ribeiro, M.G. (2017). Multidrug resistant bacteria isolated from septic arthritis in horses. Pesq. Vet. Bras. 37(4):325-330. DOI: 10.1590/S0100-736X2017000400005
• Mulon, P.-Y., Desrochers, A., & Francoz, D., (2016a). Surgical management of septic arthritis. Vet. Clin. North Am. Food Anim. Pract. 32: 777–795. https://doi.org/10.1016/j. cvfa.2016.05.014.
• Oktem, B., Anteplioglu, H. (1967). Evcil hayvanların artritis prulentalarının lokal olarak penisilin enjeksiyonlarıyla tedavisi üzerine araştırmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 14(3), 321-343. https://doi.org/10.1501/Vetfak_0000002052
• Otlu, S. (1996). Kars yöresinde koyun pnömonilerinden mikoplazmaların izolasyonu, identifikasyonu ve antibiyotiklere olan duyarlılıklarının belirlenmesi. PhD Dissertation, Kafkas University, Kars, Turkey (in Turkish).
• Ozaydin, I. (1990). Eklem hastalıklarının tanısında sinoviyal sıvı bulgularının önemi. Veteriner Hekimler Derneği Dergisi, 60(1-2), 35-42. (in Turkish)
• Power, H.T., & Rebhun, W.C. (1983). Bacterial endocarditis in adult dairy cattle. Journal of American Veterinary Medical Association, 182(8), 806–808. PMID: 6343319.
• Pritchard, D.G., Shreeve, J., & Bradley, R. (1979). Experimental infection of calves with a British strain of Haemophilus somnus. Research in Vaterinary Science, 26(1), 7–11. PMID: 472490
• Quinn, P.J., Carter, M.E., Markey, B., & Carter, G.R. (1994). Clinical Veterinary Microbiology. 118-155, Mosby-Wolfe, London.
• Ribeiro, M.G., Risseti, R.M., Bolaños, C.A.D., Caffaro, K.A., Morais, A.C.B., Lara, G.H.B., Zamprogna, T.O., Paes, A.C., Listoni, F.J.P. & Franco, M.M.J. (2015). Trueperella pyogenes multispecies infections in domestic animals: a retrospective study of 144 cases (2002-2012). Vet. Quart. 35:1-6.
• Riley, C.B., & Farrow, C.S. (1998). Partial carpal arthrodesis in a calf with chronic infectious arthritis of the carpus and osteomyelitis of the carpal and metacarpal bones. The Canadian Veterinary Journal, 39, 438-441. PMID: 9759514
• Rohde, C., Anderson, D.E., Desrochers, A., St-Jean, G., Hull, B.L., & Rings, D.M. (2000). Synovial fluid analysis in cattle: A review of 130 cases. Veterinary Surgery, 29, 341-346. https://doi.org/10.1053/jvet.2000.5605.
• Sababoglu, E., Sahan Yapıcıer, Ö., Oztürk, D., Pehlivanoglu, F., Kaya, M., & Türütoglu, H. (2018). Bacterial agents isolated from calves with arthritis: a retrospective study. Veterinary Journal of Mehmet Akif Ersoy University, 3(1), 36-42. https://doi.org/10.24880/maeuvfd.400884.
• Samsar, E., & Akın, F. (2000). Genel Cerrahi. (pp.367-416), Medipres Matbaacılık, Malatya.
• Stalheim, O.H.V., & Stone, S.S. (1975). Isolation and identification of Mycoplasma agalactiae subsp. bovis from arthritic cattle in Iowa and Nebraska. Journal of Clinical Microbiology, 2, 169- 172. https://doi.org/10.1128/jcm.2.3.169-172.1975.
• Sutradhar, B.C., Biswas, S., Datta, A., Biswas, S., Chowdhury, J., & Bristi, S.Z.T. (2019). Management of septic arthritis in a calf. Bangladesh Journal of Veterinary and Animal Science, 7(2),75-79.
• Ulbegi-Mohyla, H., Hijazin, M., Alber, J., Lämmler, C., Hassan, A.A., Abdulmawjood, A., Prenger-Berninghoff, E., Weiß, R., & Zschöck, M. (2010). Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties. Journal of Veterinary Science, 11(3), 265-267. https://doi.org/10.4142/jvs.2010.11.3.265.
• Watkins, G.H. & Sharp, M.W. (1998). Bacteria isolated from arthritic and omphalitic lesions in lambs in England and Wales. Veterinary Journal, 156, 235-238. https://doi.org/10.1016/S1090-0233(98)80132-9.
• Yurdakul, I. (2018). Evaluation of clinical, radiological, ultrasonographic and microbiological findings of septic arthritis in 50 calves. Revista Mexicana de Ciencias Pecuarias, 10(1), 254-266. http://dx.doi.org/10.22319/rmcp.v10i1.4727.
• Yurdakul, I., & Apaydın Yıldırım, B. (2019). Evaluation of total antioxidant capacity and total oxidant status, malondialdehyde levels in cases of arthritis and intestinal atresia, fracture, omphalitis of newborn calves.. 4th International Scientific research Congress, 14-17 February, Yalova.