IN VITRO EVALUATION OF ELECTROSPUN POLYSACCHARIDE BASED NANOFIBROUS MATS AS SURGICAL ADHESION BARRIERS

Year 2020, Volume: 30 Issue: 2, 99 - 107, 28.06.2020

Şerife Şafak Özgür Vatan Nilüfer Çinkılıç Esra Karaca

https://doi.org/10.32710/tekstilvekonfeksiyon.548460

Abstract

Postoperative adhesions are one of the most important
problems that the patients and surgeons face. In this study, nanofibrous mats
as a novel surgical adhesion barrier were produced from polysaccharide-based
polymers, hyaluronic acid, carboxymethyl cellulose and sodium alginate, via
electrospinning. The produced nanofibrous mats were crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride and
N-hydroxysulfosuccinimide. Furthermore, the morphology, and the in vitro degradation, cytotoxicity and
cell adherence potential of the nanofibrous mats aimed to be used as adhesion
barriers were evaluated and compared with a commercial adhesion barrier. After the crosslinking process,
the nanofibrous structure was maintained, and the diameters of resulting
nanofibers were below 300 nm. Results of the in vitro experiment showed that the
nanofibrous mats have maintained their physical structures during the critical
period for adhesion formation, and had non-adherent cell feature and non-cytotoxic
nature required for an ideal adhesion barrier.

Keywords

nanofiber, adhesion barrier, polysaccharide polymers, , in vitro

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