KANTİTATİF TAYİNDE YEŞİL YÖNTEM:ATR-FTIR; FARMASÖTİK PREPARATTA PARASETAMOL TAYİNİ

Year 2023, Volume: 22 Issue: 44, 425 - 433, 31.12.2023

Nükte Topraksever Gamze Özgül Artuç

https://doi.org/10.55071/ticaretfbd.1375697

Abstract

Bu çalışmada azaltılmış toplam yansıma Fourier dönüşümlü kızıl ötesi (ATR-FTIR) spektroskopisi kullanılarak farmasötik preparatta parasetamol tayin edilmiştir. Parasetamol standart konsantrasyonları NaCl ile seyreltilerek % 20 ile % 100 (a/a) arasında hazırlanmıştır. Parasetamol standardının FTIR spektrumu alınarak karakteristik C=O pikinin değişen yüzde geçirgenliği kullanılarak kalibrasyon eğrisi çizilmiştir. Regresyon katsayısı (R2) 0.9993 ile iyi bir doğrusallık göstermiştir. Parasetamol içeren ticari farmasötik preparattaki miktar tayini yapılmıştır. Farmasötik preparattaki parasetamol miktar tayininin geri kazanımı  % 100’dür. Farmasötik preparata uygulanan ATR-FTIR yöntemi parasetamolün rutin tayini için uygundur.

Keywords

ATR-FTIR, Farmasötik preparat, Parasetamol, Yeşil Kimya

GREEN METHOD FOR QUANTITATIVE DETERMINATION: ATR-FTIR; DETERMINATION OF PARACETAMOL IN PHARMACEUTICAL PREPARATION

Abstract

In this study, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy was used for the determination of paracetamol in pharmaceutical preparation. Paracetamol standard concentrations were prepared between 20% and 100% (w/w) by dilution with NaCl. The FTIR spectrum of the paracetamol standard was taken and the calibration curve was plotted using the varying percentage transmittance of the characteristic C=O peak. This curve showed good linearity with a regression coefficient (R2) of 0,9993. Quantification was carried out in commercial pharmaceutical preparation containing paracetamol. The recovery of the quantification of paracetamol in pharmaceutical preparation is  100%. The ATR-FTIR method applied to pharmaceutical preparation is suitable for the routine determination of paracetamol.

Keywords

ATR-FTIR, Pharmaceutical preparation, Paracetamol, Green Chemistry

References

• Alsaad, A. A. A., Alassadi, E. A. S., Al-Salman, H. N. K. & Hussein, H. H. (2019). The simultaneous determination of ibuprofen and paracetamol in pharmaceutical formulations by high-performance liquid chromatography with ultraviolet detection. Asian Journal of Pharmaceutics 13(2) 141-152.
• Boltia, S. A., Soudi, A. T., Elzanfaly, E. S. & Zaazaa, H. E. (2020). Development and validation of chromatographic methods for simultaneous determination of paracetamol, orphenadrine citrate and caffeine in presence of p-aminophenol; quantification of p-aminophenol nephrotoxic impurity using LC-MS/MS. Journal of Chromatographic Science, 58(3), 223-233. https://doi.org/10.1093/chromsci/bmz094.
• Bosch, M. E., Sánchez, A. J. R., Rojas, F. S. & Ojeda, C. B. (2006). Determination of paracetamol: Historical evolution. Journal of Pharmaceutical and Biomedical Analysis 42(3) 291-321. https://doi.org/10.1016/j.jpba.2006.04.007.
• Dinç, E., Ertekin, Z. C. & Ünal, N. (2020). Three-way analysis of pH-UV absorbance dataset for the determination of paracetamol and its pKa value in presence of excipients. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 230. https://doi.org/10.1016/j.saa.2020.118049.
• Doğan, B., Elik, A. & Altunay, N. (2020). Determination of paracetamol in synthetic urea and pharmaceutical samples by shaker-assisted deep eutectic solvent microextraction and spectrophotometry. Microchemical Journal, 154. https://doi.org/10.1016/j.microc.2020.104645.
• Dong, N. T., Trang, N. T. T., Hoang, N. V., Linh, P. T. & Yen, P. T. (2023). Simultaneous determination of paracetamol and diclofenac in wastewater by high-performance liquid chromatography method. Vietnam Journal of Science and Technology, 61(4), 599-608. https://doi.org/10.15625/2525-2518/16418.
• Han, D. (2023). Simultaneous determination of paracetamol, propyphenazone, aspirin and caffeine in white wine samples by liquid chromatography-triple quadrupole tandem mass spectrometry. Research Square, https://doi.org/10.21203/rs.3.rs-2794814/v1.
• Hasan, M. H., El-sadek, M. E. & El-Adl, S. M. (2021). The using of dansyl chloride for novel spectrofluorimetric determination of paracetamol in tablet dosage form. Analytical Chemistry Letters, 11(3), 427-436. https://doi.org/10.1080/22297928.2021.1925586.
• Hejaz, H. A., Kanan, A., Al Mohtaseb, M. & Ja’bari, A. (2020). Development and characterization of paracetamol medicated lollipops. Pharmaceutical Technology in Hospital Pharmacy, 5(1). https://doi.org/10.1515/pthp-2020-0012.
• Husain, F., Ysrafil, Y., Daud, R. P. A., Aisyah, A. N., Fadri, A. & Nur, S. (2023). Authentication of medicinal chemicals in traditional herbal products (Jamu) by UV-Vis spectrophotometry. Hacettepe University Journal of the Faculty of Pharmacy, 43(2), 100-110. https://doi.org/10.52794/hujpharm.1090673.
• ICH (2014). Harmonised Tripartite Guideline Validation Of Analytical Procedures: Text And Methodology Q2(R1) The international conference on harmonisation of technical requirements for registration of pharmaceuticals for human use. www.somatek.com adresinden 12 Ekim 2023 tarihinde alınmıştır.
• Kokilambigai, K. S. & Lakshmi, K. S. (2021). Utilization of green analytical chemistry principles for the simultaneous estimation of paracetamol, aceclofenac and thiocolchicoside by UV spectrophotometry. Green Chemistry Letters and Reviews, 14(1) 97-105. https://doi.org/10.1080/17518253.2020.1862311.
• Mahesar, S. A., Sherazi, S. T. H., Kandhro, A. A., Bhanger, M. I., Khaskheli, A. R. & Talpur, M.Y. (2011). Evaluation of important fatty acid ratios in poultry feed lipids by ATR FTIR spectroscopy. Vibrational Spectroscopy, 57(2), 177-181. https://doi.org/10.1016/j.vibspec.2011.06.009.
• Mallah, M. A., Sherazi, S. T. H., Mahesar, S. A. & Khaskheli, A. R. (2012). Simultaneous quantification of ibuprofen and paracetamol in tablet formulations using transmission fourier transform infrared spectroscopy. American Journal of Analytical Chemistry, 03(08), 503-511. https://doi.org/10.4236/ajac.2012.38067.
• Nugrahani, I. & Khalida, F. N. (2018). Green method for act and ibuprofen simultaneous assay in the combination tablet using FTIR. International Journal of Applied Pharmaceutics, 10(3), 77-85. https://doi.org/10.22159/ijap.2018v10i3.23034.
• Pasha, C. (2020). Determination of paracetamol in pharmaceutical samples by spectrophotometric method. Ecletica Quimica, 45(3), 37-46. https://doi.org/10.26850/1678-4618eqj.v45.3.2020.p37-46.
• Pereira, F. J., Rodríguez-Cordero, A., López, R., Robles, L. C. & Aller, A. J. (2021). Development and validation of an rp-hplc-pda method for determination of paracetamol, caffeine and tramadol hydrochloride in pharmaceutical formulations. Pharmaceuticals, 14(5). https://doi.org/10.3390/ph14050466.
• Qasim, B. H. (2010). Quantitative Determination of Paracetamol in Pharmaceutical Formulations by FTIR Spectroscopy. Eng. & Tech. Journal, 28(15). www.pdffactory.com adresinden 12 Ekim 2023 tarihinde alınmıştır.
• Sabbagh, B. A., Kumar, P. V., Chew, Y. L., Chin, J. H. & Akowuah, G. A. (2022). Determination of metformin in fixed-dose combination tablets by ATR-FTIR spectroscopy. Chemical Data Collections, 39. https://doi.org/10.1016/j.cdc.2022.100868.
• Saeed, A. M., Mohammed, O. J. & Hussein, N. G. (2023). Validation of liquid chromatographic analytical method for determination of aspirin, caffeine and paracetamol in some pharmaceutical tablets form in Iraqi market. Research Journal of Pharmacy and Technology, 16(1), 215-220. https://doi.org/10.52711/0974-360X.2023.00040.
• SDBS, (2023) https://sdbs.db.aist.go.jp/sdbs/cgi-bin/direct_frame_top.cgi adresinden 12 Ekim 2023 tarihinde alınmıştır.
• Sherazi, S. T. H., Ali, M. & Mahesar, S. A. (2011). Application of Fourier-transform infrared (FT-IR) transmission spectroscopy for the estimation of roxithromycin in pharmaceutical formulations. Vibrational Spectroscopy, 55(1), 115-118. https://doi.org/10.1016/j.vibspec.2010.09.010.
• Shishov, A., Bulatov, A., Locatelli, M., Carradori, S. & Andruch, V. (2017). Application of deep eutectic solvents in analytical chemistry. A review. Microchemical Journal, 135, 33-38. https://doi.org/10.1016/j.microc.2017.07.015.
• Vichare, V., Mujgond, P. & Tambe, V. (2010). Simultaneous Spectrophotometric determination of paracetamol and caffeine in tablet formulation. International Journal of PharmTech Research CODEN 2(4).