Research Article
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Year 2018, Volume: 2 Issue: 1, 26 - 33, 30.04.2018

Abstract

References

  • Ministry of Health. (2014). Türkiye diyabet programı 2015-2020, T.C. Sağlık Bakanlığı, Türkiye Halk Sağlığı Kurumu Ankara, 2. Basım: Ekim 2014.2.
  • Paneni F, Beckman JA, Creager MA, Cosentino F. Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: part I. Eur Heart J 2013; 34(31):2436–2443.3.
  • Kanter M, Coskun O, Korkmaz A, Oter S. Effects of Nigella sativa on oxidative stress and β-cell damage in streptozotocin-induced diabetic rats. Anat Rec A Discov Mol Cell Evol Biol 2004; 279(1):685-691.4.
  • Kajimoto Y, Kaneto H. Role of oxidative stress in pancreatic beta-cell dysfunction. Ann NY Acad Sci 2004; 1011:168–176.5.
  • Rahimi-Madiseh M, Malekpour-Tehrani A, Bahmani M, Rafieian-Kopaei M. The research and development on the antioxidants in prevention of diabetic complications. Asian Pac J Trop Med 2016; 9(9):825-831.6.
  • Demirci F, Berber H, İşcan G. Biyokatalizörler yardımıyla p-simen’den timokinon ve benzeri biyoaktif metabolitlerin üretimi. Temel Bilimler Araştırma Grubu, Proje No: 106T117, 2008. Eskişehir.7.
  • Pari L, Sankaranarayanan C. Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin–nicotinamide induced diabetic rats. Life Sci 2009; 85(23-26):830-834.8.
  • Bourgou S, Bettaieb I, Saidani M, Marzouk B. Fatty Acids, Essential Oil, And Phenolics Modifications of Black Cumin Fruit under NaCl Stress Conditions. J Agric Food Chem 2010; 58(23):12399–12406.9.
  • Abdelmeguid NE, Fakhoury R, Kamal SM, AL Wafa RJ. Effects of nigella sativa and thymoquinone on biochemical and subcellular changes in pancreatic β-cells of streptozotocin-induced diabetic rats. J Diabetes 2010; 2(4):256–266.10.
  • Kanter M. Protective effects of thymoquinone on streptozotocin-induced diabetic nephropathy. J Mol Histol 2009; 40(2):107–115.11.
  • Yüksek Ş. Nigella Sativa Sulu Ekstresinin Oksidatif DNA Hasarına Etkileri, Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi 2011; Afyonkarahisar.
  • Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total Phenolics and flavonoids Content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules 2010; 15(6):4324-4333.13.
  • Gökpınar Ş, Koray T, Akçiçek E, Göksan T, Durmaz Y. Algal Antioksidanlar. EgeJFAS 2006; 23(1/1):85-89.14. Meerza D, Iqbal S, Zaheer S, Naseem I. Retinoids have therapeutic action in type 2 diabetes. Nutrition 2016; 32(7-8):898-903.15.
  • Joergensen C, Gall MA, Schmedes A, Tarnow L, Parving HH, et al. Vitamin D levels and mortality in type 2 diabetes. Diabetes Care 2010; 33(10):2238-2243.16.
  • Mathieu C, Gysemans C, Giulietti A, Bouillon R. Vitamin D and diabetes. Diabetologia 2005; 48(7):1247-1257.17.
  • Chiu KC, Chu A, Go VL, Saad MF. Hypovitaminosis D is associated with insulin resistance and beta cell dysfunction. Am J Clin Nutr 2004; 79(5):820-825.18.
  • Chan KM, Decker EA. Endogenous skeletal muscle antioxidants. Crit Rev Food Sci Nutr 1994; 34(4):403-426.19.
  • Vardı N, Iraz M, Öztürk F, et al. Deneysel diyabetin sıçan böbreklerinde meydana getirdiği histolojik değişiklikler üzerine melatoninin iyileştirici etkileri. İnönü Üniv Tıp Fak Derg 2005; 12(3):145-152.20.
  • Miller KW, Yang CS. An Isocratic High-Performance Liquid Chromatography Method for the Simultaneous Analysis of Plasma Retinol, α-tocopherol and Various Carotenoids. Anal Biochem 1985; 145(1):21-26.21.
  • Zaspel BJ, Csallany AS. Determination of Alpha-Tocopherol in Tissues and Plasma by High-Performance Liquid Chromatography. Analytical Biochemistry 1983; 130(1):146-150.22.
  • Reynolds SL, Judd HJ. Rapid Procedure for the Determination of Vitamins A and D in Fortified Skimmed Milk Powder Using High-Performance Liquid Chromatography. Analyst 1984; 109(4):489-492.23.
  • Usta A. Deneysel diyabetli ratlarda timokinon uygulanmasının nükleer faktör kappa B (Nf-κB) ve DNA hasarı üzerine etkisi, Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Biyokimya Anabilim Dalı, Doktora tezi 2014; Van.24.
  • Abukhader MM. Thymoquinone: a promising antidiabetic agent. Int J Diabetes Dev Ctries 2012; 32(2):65–68.25.
  • Yonca SS. Timokinonun streptozotosin ile oluşturulan tip 1 diyabette damar hasarı üzerine etkisi. Fırat Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Farmakoloji Anabilim Dalı, Yüksek Lisans Tezi 2015; Elazığ.26.
  • Liu H, Liu HY, Jiang YN, Li N. Protective effect of thymoquinone improves cardiovascular function, and attenuates oxidative stress, inflammation and apoptosis by mediating the PI3K/Akt pathway in diabetic rats. Mol Med Rep 2016; 13(3):2836-2842.27.
  • Fouad AA, Alwadani F. Ameliorative effects of thymoquinone against eye lens changes in streptozotocin diabetic rats. Environ Toxicol Pharmacol 2015; 40(3):960-965. 28.
  • Sezen S. Timokinonun insan hücre hattı modelinde insülin sinyal yolaklarına etkilerinin moleküler düzeyde araştırılması. Erciyes Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji Anabilim Dalı, Yüksek Lisans Tezi 2015; Kayseri.29.
  • Kutlu M, Naziroğlu M, Simşek H, Yilmaz T, Sahap Kükner A. Moderate exercise combined with dietary vitamins C and E counteracts oxidative stress in the kidney and lens of streptozotocin-induced diabetic-rat. Int J Vitam Nutr Res 2005; 75(1):71-80.30.
  • Lipinski B. Pathophysiology of oxidative stress in diabetes mellitus. Journal of Diabetes and Its Complications 2001; 15:203–210.31. Z
  • ujko ME, Witkowska AM, Górska M, Wilk J, Krętowski A. Reduced intake of dietary antioxidants can impair antioxidant status in type 2 diabetes patients. Pol Arch Med Wewn 2014; 124(11): 599-607.32.
  • Sankaranarayanan C, Pari L. Thymoquinone ameliorates chemical induced oxidative stress and β-cell damage in experimental hyperglycemic rats. Chem Biol Interact 2011; 190( 2-3):148-154.33.
  • Gren A. Effects of vitamin E, C and D supplementation on inflammation and oxidative stress in streptozotocin-induced diabetic mice. Int J Vitam Nutr Res 2013; 83(3):168-175.34.
  • Rağbetli C, Dede S, Tanritanir P, Yoruk IH, Rağbetli MC. Determination of micronutrients and oxidative stress status in the blood of STZ-induced experimental diabetic rat models. Cell Biochem Biophys 2014; 70(2): 933-93835.
  • Yosaee S, Akbari Fakhrabadi M, Shidfar F. Positive evidence for vitamin A role in prevention of type 1 diabetes. World J Diabetes 2016; 7(9):177-188.36.
  • Nyomba BL, Bouillon R, De Moor P. Influence of vitamin D status on insulin secretion and glucose tolerance in the rabbit. Endocrinology 1984; 115(1):191-197.37.
  • Mezza T, Muscogiuri G, Sorice GP, et al. Vitamin D deficiency: a new risk factor for type 2 diabetes? Ann Nutr Metab 2012; 61(4):337-348.38.
  • Sharma A, Kharb S, Chugh SN, Kakkar R, Singh GP. Singh Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients. Metabolism 2000; 49(2):160-162.39.
  • Rafraf M, Bazyun B, Sarabchian MA, Safaeiyan A, Gargari BP. Vitamin E Improves Serum Paraoxonase-1 Activity and Some Metabolic Factors in Patients with Type 2Diabetes: No Effects on Nitrite/Nitrate Levels. J Am Coll Nutr 2016; 35(6):521-528.40.
  • Farid N, Inbal D, Nakhoul N, Evgeny F, Miller-Lotan R et al. Vitamin E and diabetic nephropathy in mice model and humans. World J Nephrol 2013; 2(4):111-124.

The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats

Year 2018, Volume: 2 Issue: 1, 26 - 33, 30.04.2018

Abstract

Objectives: Thymoquinone, the basic bioactive phytochemical constituent of seeds oil of Nigella sativa,
is one of these herbal drugs with known antidiabetic effects. In this study, effects of thymoquinone (TQ)
on antioxidant vitamin levels in experimental diabetic rats were investigated.

Materials and Methods:
Twenty-eight male Wistar-Albino rats (200-250 g) were used as material. The rats were divided into
four groups of control (C), thymoquinone (T), diabetes (D) and diabetes + thymoquinone (DT). D and
DT groups were treated with 45 mg/kg streptozotocin (STZ) (i.p). TQ was administered as 30 mg/kg/21
days by oral gavage in DT and T groups.

Results: The retinol concentrations were observed to have significantly decreased in the diabetes and
diabetes + TQ groups (p ≤ 0.05). There was no difference between the group delivered TQ and the
control group. It was identified that the tocopherol concentrations were lower in all study groups than
in the control group, and it was identified that the reduction in the diabetes and diabetes + TQ groups
was significant when compared to the control group (p ≤ 0.05). It was identified that the vitamin D3
levels were significantly lower in the study groups when compared to the control group (p ≤ 0.05).

Conclusion: It was identified that the serum antioxidant vitamin levels decreased in the group with
experimental diabetes and that TQ administration did not significantly affect the antioxidant vitamin
levels significantly in the group with experimental diabetes.

References

  • Ministry of Health. (2014). Türkiye diyabet programı 2015-2020, T.C. Sağlık Bakanlığı, Türkiye Halk Sağlığı Kurumu Ankara, 2. Basım: Ekim 2014.2.
  • Paneni F, Beckman JA, Creager MA, Cosentino F. Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: part I. Eur Heart J 2013; 34(31):2436–2443.3.
  • Kanter M, Coskun O, Korkmaz A, Oter S. Effects of Nigella sativa on oxidative stress and β-cell damage in streptozotocin-induced diabetic rats. Anat Rec A Discov Mol Cell Evol Biol 2004; 279(1):685-691.4.
  • Kajimoto Y, Kaneto H. Role of oxidative stress in pancreatic beta-cell dysfunction. Ann NY Acad Sci 2004; 1011:168–176.5.
  • Rahimi-Madiseh M, Malekpour-Tehrani A, Bahmani M, Rafieian-Kopaei M. The research and development on the antioxidants in prevention of diabetic complications. Asian Pac J Trop Med 2016; 9(9):825-831.6.
  • Demirci F, Berber H, İşcan G. Biyokatalizörler yardımıyla p-simen’den timokinon ve benzeri biyoaktif metabolitlerin üretimi. Temel Bilimler Araştırma Grubu, Proje No: 106T117, 2008. Eskişehir.7.
  • Pari L, Sankaranarayanan C. Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin–nicotinamide induced diabetic rats. Life Sci 2009; 85(23-26):830-834.8.
  • Bourgou S, Bettaieb I, Saidani M, Marzouk B. Fatty Acids, Essential Oil, And Phenolics Modifications of Black Cumin Fruit under NaCl Stress Conditions. J Agric Food Chem 2010; 58(23):12399–12406.9.
  • Abdelmeguid NE, Fakhoury R, Kamal SM, AL Wafa RJ. Effects of nigella sativa and thymoquinone on biochemical and subcellular changes in pancreatic β-cells of streptozotocin-induced diabetic rats. J Diabetes 2010; 2(4):256–266.10.
  • Kanter M. Protective effects of thymoquinone on streptozotocin-induced diabetic nephropathy. J Mol Histol 2009; 40(2):107–115.11.
  • Yüksek Ş. Nigella Sativa Sulu Ekstresinin Oksidatif DNA Hasarına Etkileri, Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi 2011; Afyonkarahisar.
  • Ghasemzadeh A, Jaafar HZE, Rahmat A. Antioxidant activities, total Phenolics and flavonoids Content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules 2010; 15(6):4324-4333.13.
  • Gökpınar Ş, Koray T, Akçiçek E, Göksan T, Durmaz Y. Algal Antioksidanlar. EgeJFAS 2006; 23(1/1):85-89.14. Meerza D, Iqbal S, Zaheer S, Naseem I. Retinoids have therapeutic action in type 2 diabetes. Nutrition 2016; 32(7-8):898-903.15.
  • Joergensen C, Gall MA, Schmedes A, Tarnow L, Parving HH, et al. Vitamin D levels and mortality in type 2 diabetes. Diabetes Care 2010; 33(10):2238-2243.16.
  • Mathieu C, Gysemans C, Giulietti A, Bouillon R. Vitamin D and diabetes. Diabetologia 2005; 48(7):1247-1257.17.
  • Chiu KC, Chu A, Go VL, Saad MF. Hypovitaminosis D is associated with insulin resistance and beta cell dysfunction. Am J Clin Nutr 2004; 79(5):820-825.18.
  • Chan KM, Decker EA. Endogenous skeletal muscle antioxidants. Crit Rev Food Sci Nutr 1994; 34(4):403-426.19.
  • Vardı N, Iraz M, Öztürk F, et al. Deneysel diyabetin sıçan böbreklerinde meydana getirdiği histolojik değişiklikler üzerine melatoninin iyileştirici etkileri. İnönü Üniv Tıp Fak Derg 2005; 12(3):145-152.20.
  • Miller KW, Yang CS. An Isocratic High-Performance Liquid Chromatography Method for the Simultaneous Analysis of Plasma Retinol, α-tocopherol and Various Carotenoids. Anal Biochem 1985; 145(1):21-26.21.
  • Zaspel BJ, Csallany AS. Determination of Alpha-Tocopherol in Tissues and Plasma by High-Performance Liquid Chromatography. Analytical Biochemistry 1983; 130(1):146-150.22.
  • Reynolds SL, Judd HJ. Rapid Procedure for the Determination of Vitamins A and D in Fortified Skimmed Milk Powder Using High-Performance Liquid Chromatography. Analyst 1984; 109(4):489-492.23.
  • Usta A. Deneysel diyabetli ratlarda timokinon uygulanmasının nükleer faktör kappa B (Nf-κB) ve DNA hasarı üzerine etkisi, Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Biyokimya Anabilim Dalı, Doktora tezi 2014; Van.24.
  • Abukhader MM. Thymoquinone: a promising antidiabetic agent. Int J Diabetes Dev Ctries 2012; 32(2):65–68.25.
  • Yonca SS. Timokinonun streptozotosin ile oluşturulan tip 1 diyabette damar hasarı üzerine etkisi. Fırat Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Farmakoloji Anabilim Dalı, Yüksek Lisans Tezi 2015; Elazığ.26.
  • Liu H, Liu HY, Jiang YN, Li N. Protective effect of thymoquinone improves cardiovascular function, and attenuates oxidative stress, inflammation and apoptosis by mediating the PI3K/Akt pathway in diabetic rats. Mol Med Rep 2016; 13(3):2836-2842.27.
  • Fouad AA, Alwadani F. Ameliorative effects of thymoquinone against eye lens changes in streptozotocin diabetic rats. Environ Toxicol Pharmacol 2015; 40(3):960-965. 28.
  • Sezen S. Timokinonun insan hücre hattı modelinde insülin sinyal yolaklarına etkilerinin moleküler düzeyde araştırılması. Erciyes Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji Anabilim Dalı, Yüksek Lisans Tezi 2015; Kayseri.29.
  • Kutlu M, Naziroğlu M, Simşek H, Yilmaz T, Sahap Kükner A. Moderate exercise combined with dietary vitamins C and E counteracts oxidative stress in the kidney and lens of streptozotocin-induced diabetic-rat. Int J Vitam Nutr Res 2005; 75(1):71-80.30.
  • Lipinski B. Pathophysiology of oxidative stress in diabetes mellitus. Journal of Diabetes and Its Complications 2001; 15:203–210.31. Z
  • ujko ME, Witkowska AM, Górska M, Wilk J, Krętowski A. Reduced intake of dietary antioxidants can impair antioxidant status in type 2 diabetes patients. Pol Arch Med Wewn 2014; 124(11): 599-607.32.
  • Sankaranarayanan C, Pari L. Thymoquinone ameliorates chemical induced oxidative stress and β-cell damage in experimental hyperglycemic rats. Chem Biol Interact 2011; 190( 2-3):148-154.33.
  • Gren A. Effects of vitamin E, C and D supplementation on inflammation and oxidative stress in streptozotocin-induced diabetic mice. Int J Vitam Nutr Res 2013; 83(3):168-175.34.
  • Rağbetli C, Dede S, Tanritanir P, Yoruk IH, Rağbetli MC. Determination of micronutrients and oxidative stress status in the blood of STZ-induced experimental diabetic rat models. Cell Biochem Biophys 2014; 70(2): 933-93835.
  • Yosaee S, Akbari Fakhrabadi M, Shidfar F. Positive evidence for vitamin A role in prevention of type 1 diabetes. World J Diabetes 2016; 7(9):177-188.36.
  • Nyomba BL, Bouillon R, De Moor P. Influence of vitamin D status on insulin secretion and glucose tolerance in the rabbit. Endocrinology 1984; 115(1):191-197.37.
  • Mezza T, Muscogiuri G, Sorice GP, et al. Vitamin D deficiency: a new risk factor for type 2 diabetes? Ann Nutr Metab 2012; 61(4):337-348.38.
  • Sharma A, Kharb S, Chugh SN, Kakkar R, Singh GP. Singh Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients. Metabolism 2000; 49(2):160-162.39.
  • Rafraf M, Bazyun B, Sarabchian MA, Safaeiyan A, Gargari BP. Vitamin E Improves Serum Paraoxonase-1 Activity and Some Metabolic Factors in Patients with Type 2Diabetes: No Effects on Nitrite/Nitrate Levels. J Am Coll Nutr 2016; 35(6):521-528.40.
  • Farid N, Inbal D, Nakhoul N, Evgeny F, Miller-Lotan R et al. Vitamin E and diabetic nephropathy in mice model and humans. World J Nephrol 2013; 2(4):111-124.
There are 39 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section 2018 Volume 2 Number 1
Authors

Ayse Usta

Semiha Dede

Ibrahim Hakki Yoruk 0000-0002-0525-0346

Publication Date April 30, 2018
Submission Date March 5, 2018
Published in Issue Year 2018 Volume: 2 Issue: 1

Cite

APA Usta, A., Dede, S., & Yoruk, I. H. (2018). The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats. Turkish Journal of Veterinary Research, 2(1), 26-33.
AMA Usta A, Dede S, Yoruk IH. The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats. TJVR. April 2018;2(1):26-33.
Chicago Usta, Ayse, Semiha Dede, and Ibrahim Hakki Yoruk. “The Effect of Thymoquinone on Serum Antioxidant Vitamin Levels in Diabetic Rats”. Turkish Journal of Veterinary Research 2, no. 1 (April 2018): 26-33.
EndNote Usta A, Dede S, Yoruk IH (April 1, 2018) The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats. Turkish Journal of Veterinary Research 2 1 26–33.
IEEE A. Usta, S. Dede, and I. H. Yoruk, “The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats”, TJVR, vol. 2, no. 1, pp. 26–33, 2018.
ISNAD Usta, Ayse et al. “The Effect of Thymoquinone on Serum Antioxidant Vitamin Levels in Diabetic Rats”. Turkish Journal of Veterinary Research 2/1 (April 2018), 26-33.
JAMA Usta A, Dede S, Yoruk IH. The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats. TJVR. 2018;2:26–33.
MLA Usta, Ayse et al. “The Effect of Thymoquinone on Serum Antioxidant Vitamin Levels in Diabetic Rats”. Turkish Journal of Veterinary Research, vol. 2, no. 1, 2018, pp. 26-33.
Vancouver Usta A, Dede S, Yoruk IH. The effect of thymoquinone on serum antioxidant vitamin levels in diabetic rats. TJVR. 2018;2(1):26-33.