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Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride

Year 2020, Volume: 4 Issue: 2, 45 - 49, 30.10.2020

Abstract

Aim: The aim of this study was to determine the effects of lycopene administration as a protective agent against necrotic damage of NaF, a fluorine compound found to have high cytotoxic effects in the renal epithelial cell.
Material- Method: The renal epithelial cell was replicated in DMEM high glucose medium, containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin / streptomycin. With the MTT viability test, the non-toxic dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study groups were divided into four as control, NaF, lycopene and NaF + lycopene (the combination of NaF and lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of the identified necrotic genes were determined by real-time PCR method.
Results: While the Ripk 1 gene did not change in the group given lycopene at the 24th hour, it was found that it increased 2.6 times in the group that received only fluorine, while it increased 7 times in the group treated with NaF+lycopene. A significant difference was detected between the groups in terms of gene expression pattern. While the Ripk 3 gene increased slightly in the 24th hour applied lycopene group, it was observed that only lycopene applied group increased 8 times and NaF+lycopene applied group increased in the 9 times
Conclusion: Based on the results obtained from this study, it was seen that activation of necrotic genes is important in explaining the molecular basis of cell death from NaF, which is applied as fluorine source, in revealing the molecular basis of the necrotic pathway. It was found that the decrease in cell viability due to NaF increased with lycopene, but the use of lycopene with fluorine also increased necrotic gene expression.

Keywords: NaF, in vitro, Lycopene, Necrotic Genes

References

  • Adamek E, Pawłowska-Goral K, Bober K. In vitro and in vivo effects of fluoride ions on enzyme activity. Ann Acad Med Stetin 2005; 51(2): 69–85.
  • Agalakova NI, Gusev GP. Molecular mechanisms of cytotoxicity and apoptosis induced by inorganic fluoride. Cell Biology 2012, Article ID 403835.
  • Bramley PM. Is lycopene benefcial to human health? Phytochemistry 2000;54 (3): 233- 236.
  • Cao J, Chen J, Xie L, Wang J, Feng C, Song J. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway. Aquat Toxicol 2015;167,180-90.
  • Cetin S, Yur F, Taşpınar M, Dede S, Yüksek V. The effects of lycopene application on sodium fluoride (NaF) applied renal cell line. Int J Sec Metabolite 2017; 4 (3): 508-511.
  • Cetin S,Yur F, Taşpınar M, Yüksek V. The effect of some minerals on apoptosis and DNA damage in sodium fluoride-administered renal and osteoblast cell line. Fluoride 2019;52(3(Pt 2):362-378.
  • Cetin S, Değer Y, Dede S, Yur F. The concentration of certain trace elements in the wool of sheep with fluorosis. Fluoride 2020, 53(1 Pt 2):164-169.
  • Dharmaratne RW. Fluoride in drinking water and diet: the causative factor of chronic kidney diseases in the North Central Province of Sri Lanka. Environ Health Prev Med 2015;20(4):237-242.
  • Golstein P, Kroemer G. Cell death by necrosis: towards a molecular definition. Trends Biochem Sci 2007;32:37-43.
  • Hengartner MO, Ellis RE, Horvitz HR. Caenorhabditis elegans gene ced-9 protects cells from programmed cell death. Nature 1992; 356: 494-499.
  • Karube H, Nishitai G, Inageda K, Kurosu H, Matsuoka M. NaF activates MAPKs and induces apoptosis in odontoblast-like cells. J Dent Res, 2009; 88(5):461–465.
  • Kuang P, Deng H, Liu H,et al. Sodium fluoride induces splenocyte autophagy via the mammalian targets of rapamycin (mTOR) signaling pathway in growing mice. Aging (Albany NY), 2018; 10(7):1649-1665.
  • Kubota K, Lee DH, Tsuchiya M, et al. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem 2005; 280 (24): 23194– 23202.
  • Lu J, Chen H, Xu Q, et al.Comparative proteomics of kidney samples from puffer fish Takifugu rubripes exposed to excessive fluoride: an insight into molecular response to fluorosis. Toxicol Mech Methods, 2010;20 (6):345–354.
  • Mashima R, Witting PK, Stocker R. Oxidants and antioxidants in atherosclerosis. Curr Opin Lipidol 2001; 12(4):411-418.
  • Mansour HH, Tawfik SS. Efficacy of lycopene against fluoride toxicity in rats. Pharm Biol 2012, 50(6):707-711.
  • Matos HR, Capelozzi VL, Gomes OF, Mascio PD, Medeiros MH. Lycopene inhibitis DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys 2011, 396,171-174.
  • Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L, et al . The effects of fluoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumour cells. Toxicol Lett 2009; 190(2):179–186.
  • Nicotera P, Bernassola F, Melino G. Regulation of the apoptosis-necrosis switch. Oncogene 2004;23:2757-2765.
  • Perumal E, Paul V, Govindarajan V, Panneerselvam L. A brief review on experimental fluorosis. Toxicol Lett 2013; 223(2), 236-51.
  • Pruthi RS, Derksen E, Gaston K. Cyclooxygenase-2 as a potential target in the prevention and treatment of genitourinary tumors, a review. J Urol 2003; 169(6), 2352-2359.
  • Quadri JA, Alam MM, Sarwar S, Singh S, Shariff A, Das TK. Fluoride induced nephrotoxicity: apoptosis, ultra structural changes and renal tubular injury in experimental animals. Ayurveda and Pharma Res 2016;4(8):91-95.
  • Rousseau EJ, Davison AJ, Dunn B. Protection by beta-carotene and related compounds againist oxygen- mediated cytotoxicity and genotixicity implications for carcinogenesis and anticarcinogenesis. Free Radic Biol Med 1992; 13(4):407-433.
  • Rzeuski R, Chlubek D, Machoy Z. Interactions between fluoride and biological free radical reactions. Fluoride 1998(1): 43–44.
  • Shashi A, Singh JP, Thapar SP. Toxic effect of fluoride on rabbit kidney. Fluoride 2002; 35(1): 38–50.
  • Tan PP, Zhou BH, Zhao WP, Jia LS, Liu J, Wang HW. Mitochondria-mediated pathway regulates C2C12 cell apoptosis induced by fluoride. Biol Trace Elem Res 2018;185(2):440-447.
  • Tu W, Zhang Q, Liu Y, et al. Fluoride induces apoptosis via inhibiting SIRT1 activity to activate mitochondrial p53 pathway in human neuroblastoma SH-SY5Y cells. Toxicol Appl Pharmacol 2018;347, 60-69.
  • Varol E, İcli A, Aksoy F, et al. Evaluation of total oxidative statusand total antioxidant capacity in patients with endemic fluorosis. Toxicol Ind Health 2013;29(2): 175–180.
  • Wang YY, Zhao BL, Li XJ, Su Z, Xi WJ. Spin trapping technique studies onactive oxygen radicals from human polymorphonuclear leukocytes during fluoride stimulated respiratory burst. Fluoride 1997;30(1):1-5.
  • Xiong X, Liu J, He W, et al. Dose effect relationship between drinking water fluoride levels and damage to liver and kidney functions in children. Environm Res, 2007; 103, 112–116.
  • Yüksek V, Dede S, Taşpınar M, Çetin S. The effects of vitamins A, D, E, and C on apoptosis and DNA damage in sodium fluoride-treated renal and osteoblast cell lines. Fluoride 2017; 50(3):300-313.
  • Zhang Y, Li W, Chi HS, Chen J, DenBesten PK. JNK/c-Jun signaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro. Matrix Biol 2007;26 (8): 633–641.
  • Zhang M, Wang A, Xia T, He P. Effects of fluoride on DNA damage, S phase cell-cycle arrest and the expression of NF-kB in primary cultured rat hippocampal neurons. Toxicol Lett 2008;179(1):1–5.
Year 2020, Volume: 4 Issue: 2, 45 - 49, 30.10.2020

Abstract

References

  • Adamek E, Pawłowska-Goral K, Bober K. In vitro and in vivo effects of fluoride ions on enzyme activity. Ann Acad Med Stetin 2005; 51(2): 69–85.
  • Agalakova NI, Gusev GP. Molecular mechanisms of cytotoxicity and apoptosis induced by inorganic fluoride. Cell Biology 2012, Article ID 403835.
  • Bramley PM. Is lycopene benefcial to human health? Phytochemistry 2000;54 (3): 233- 236.
  • Cao J, Chen J, Xie L, Wang J, Feng C, Song J. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp (Cyprinus carpio) via JNK signaling pathway. Aquat Toxicol 2015;167,180-90.
  • Cetin S, Yur F, Taşpınar M, Dede S, Yüksek V. The effects of lycopene application on sodium fluoride (NaF) applied renal cell line. Int J Sec Metabolite 2017; 4 (3): 508-511.
  • Cetin S,Yur F, Taşpınar M, Yüksek V. The effect of some minerals on apoptosis and DNA damage in sodium fluoride-administered renal and osteoblast cell line. Fluoride 2019;52(3(Pt 2):362-378.
  • Cetin S, Değer Y, Dede S, Yur F. The concentration of certain trace elements in the wool of sheep with fluorosis. Fluoride 2020, 53(1 Pt 2):164-169.
  • Dharmaratne RW. Fluoride in drinking water and diet: the causative factor of chronic kidney diseases in the North Central Province of Sri Lanka. Environ Health Prev Med 2015;20(4):237-242.
  • Golstein P, Kroemer G. Cell death by necrosis: towards a molecular definition. Trends Biochem Sci 2007;32:37-43.
  • Hengartner MO, Ellis RE, Horvitz HR. Caenorhabditis elegans gene ced-9 protects cells from programmed cell death. Nature 1992; 356: 494-499.
  • Karube H, Nishitai G, Inageda K, Kurosu H, Matsuoka M. NaF activates MAPKs and induces apoptosis in odontoblast-like cells. J Dent Res, 2009; 88(5):461–465.
  • Kuang P, Deng H, Liu H,et al. Sodium fluoride induces splenocyte autophagy via the mammalian targets of rapamycin (mTOR) signaling pathway in growing mice. Aging (Albany NY), 2018; 10(7):1649-1665.
  • Kubota K, Lee DH, Tsuchiya M, et al. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem 2005; 280 (24): 23194– 23202.
  • Lu J, Chen H, Xu Q, et al.Comparative proteomics of kidney samples from puffer fish Takifugu rubripes exposed to excessive fluoride: an insight into molecular response to fluorosis. Toxicol Mech Methods, 2010;20 (6):345–354.
  • Mashima R, Witting PK, Stocker R. Oxidants and antioxidants in atherosclerosis. Curr Opin Lipidol 2001; 12(4):411-418.
  • Mansour HH, Tawfik SS. Efficacy of lycopene against fluoride toxicity in rats. Pharm Biol 2012, 50(6):707-711.
  • Matos HR, Capelozzi VL, Gomes OF, Mascio PD, Medeiros MH. Lycopene inhibitis DNA damage and liver necrosis in rats treated with ferric nitrilotriacetate. Arch Biochem Biophys 2011, 396,171-174.
  • Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L, et al . The effects of fluoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumour cells. Toxicol Lett 2009; 190(2):179–186.
  • Nicotera P, Bernassola F, Melino G. Regulation of the apoptosis-necrosis switch. Oncogene 2004;23:2757-2765.
  • Perumal E, Paul V, Govindarajan V, Panneerselvam L. A brief review on experimental fluorosis. Toxicol Lett 2013; 223(2), 236-51.
  • Pruthi RS, Derksen E, Gaston K. Cyclooxygenase-2 as a potential target in the prevention and treatment of genitourinary tumors, a review. J Urol 2003; 169(6), 2352-2359.
  • Quadri JA, Alam MM, Sarwar S, Singh S, Shariff A, Das TK. Fluoride induced nephrotoxicity: apoptosis, ultra structural changes and renal tubular injury in experimental animals. Ayurveda and Pharma Res 2016;4(8):91-95.
  • Rousseau EJ, Davison AJ, Dunn B. Protection by beta-carotene and related compounds againist oxygen- mediated cytotoxicity and genotixicity implications for carcinogenesis and anticarcinogenesis. Free Radic Biol Med 1992; 13(4):407-433.
  • Rzeuski R, Chlubek D, Machoy Z. Interactions between fluoride and biological free radical reactions. Fluoride 1998(1): 43–44.
  • Shashi A, Singh JP, Thapar SP. Toxic effect of fluoride on rabbit kidney. Fluoride 2002; 35(1): 38–50.
  • Tan PP, Zhou BH, Zhao WP, Jia LS, Liu J, Wang HW. Mitochondria-mediated pathway regulates C2C12 cell apoptosis induced by fluoride. Biol Trace Elem Res 2018;185(2):440-447.
  • Tu W, Zhang Q, Liu Y, et al. Fluoride induces apoptosis via inhibiting SIRT1 activity to activate mitochondrial p53 pathway in human neuroblastoma SH-SY5Y cells. Toxicol Appl Pharmacol 2018;347, 60-69.
  • Varol E, İcli A, Aksoy F, et al. Evaluation of total oxidative statusand total antioxidant capacity in patients with endemic fluorosis. Toxicol Ind Health 2013;29(2): 175–180.
  • Wang YY, Zhao BL, Li XJ, Su Z, Xi WJ. Spin trapping technique studies onactive oxygen radicals from human polymorphonuclear leukocytes during fluoride stimulated respiratory burst. Fluoride 1997;30(1):1-5.
  • Xiong X, Liu J, He W, et al. Dose effect relationship between drinking water fluoride levels and damage to liver and kidney functions in children. Environm Res, 2007; 103, 112–116.
  • Yüksek V, Dede S, Taşpınar M, Çetin S. The effects of vitamins A, D, E, and C on apoptosis and DNA damage in sodium fluoride-treated renal and osteoblast cell lines. Fluoride 2017; 50(3):300-313.
  • Zhang Y, Li W, Chi HS, Chen J, DenBesten PK. JNK/c-Jun signaling pathway mediates the fluoride-induced down-regulation of MMP-20 in vitro. Matrix Biol 2007;26 (8): 633–641.
  • Zhang M, Wang A, Xia T, He P. Effects of fluoride on DNA damage, S phase cell-cycle arrest and the expression of NF-kB in primary cultured rat hippocampal neurons. Toxicol Lett 2008;179(1):1–5.
There are 33 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section 2020 Volume 4 Number 2
Authors

Sedat Çetin 0000-0002-6102-8571

Veysel Yüksek 0000-0001-7432-4989

Semiha Dede 0000-0001-5744-6327

Ayşe Usta 0000-0002-5522-3469

Mehmet Taşpınar 0000-0002-7598-2665

Publication Date October 30, 2020
Submission Date April 21, 2020
Published in Issue Year 2020 Volume: 4 Issue: 2

Cite

APA Çetin, S., Yüksek, V., Dede, S., Usta, A., et al. (2020). Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. Turkish Journal of Veterinary Research, 4(2), 45-49.
AMA Çetin S, Yüksek V, Dede S, Usta A, Taşpınar M. Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. TJVR. October 2020;4(2):45-49.
Chicago Çetin, Sedat, Veysel Yüksek, Semiha Dede, Ayşe Usta, and Mehmet Taşpınar. “Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride”. Turkish Journal of Veterinary Research 4, no. 2 (October 2020): 45-49.
EndNote Çetin S, Yüksek V, Dede S, Usta A, Taşpınar M (October 1, 2020) Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. Turkish Journal of Veterinary Research 4 2 45–49.
IEEE S. Çetin, V. Yüksek, S. Dede, A. Usta, and M. Taşpınar, “Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride”, TJVR, vol. 4, no. 2, pp. 45–49, 2020.
ISNAD Çetin, Sedat et al. “Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride”. Turkish Journal of Veterinary Research 4/2 (October 2020), 45-49.
JAMA Çetin S, Yüksek V, Dede S, Usta A, Taşpınar M. Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. TJVR. 2020;4:45–49.
MLA Çetin, Sedat et al. “Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride”. Turkish Journal of Veterinary Research, vol. 4, no. 2, 2020, pp. 45-49.
Vancouver Çetin S, Yüksek V, Dede S, Usta A, Taşpınar M. Effect of Lycopene Administration on Necrotic Gene Expression in Renal Epithelial Cell Line (NRK-52E) Exposed to Sodium Fluoride. TJVR. 2020;4(2):45-9.