Antioxidant and Antimicrobial Activities of Some Newly Synthesized 4-[1- (2,6-Dimethylmorpholin-4-yl-methyl)-3-alkyl(aryl)-4,5-dihydro-1H-1,2,4- triazol-5-on-4-yl-azomethine]-2-methoxyphenyl benzoates

Yıl 2018, Sayı: 2, 72 - 80, 19.08.2018

Yonca Yilmaz Ahmet Harmankaya Sevda Manap Haydar Yuksek Ozlem Gursoy Kol Muzaffer Alkan

Öz

In this study, 4-(3-alkyl/aryl-4,5-dihydro-1H-1,2,4-triazol-5-one-4-yl-azomethine)-2-methoxyphenyl
benzoates were treated with 2,6-dimethylmorpholine in the presence of
formaldehyde according to the Mannich reaction to synthesize six novel
4-[1-(2,6-dimethylmorpholin-4-yl-methyl)-3-alkyl(aryl)-4,5-dihydro-triazol-5-on-4-yl-azomethine]-2-methoxyphenyl
benzoates. The structures of synthesized six novel heterocyclic compounds were
characterized by IR, ¹³C-NMR and ¹H-NMR spectroscopic
methods. The novel 4-[1-(2,6-dimethylmorpholin-4-yl-methyl)-3-alkyl(aryl)-4,5-dihydro-triazol-5-on-4-yl-azomethine]-2-methoxyphenyl
benzoates were investigated in vitro
antioxidant properties by using reducing
power, free radical scavenging and metal chelating activity. For the
measurement of the reductive ability, Fe³⁺-Fe²⁺ transformation
was investigated in the presence of compound using by the method of Oyaizu (1986). The hydrogen atoms or electrons
donation ability of the synthesized compound was measured by DPPH^. using the method of Blois (1958). The chelating effect of ferrous ions by the
compound was determined according to the method of Dinis, Madeira & Almeida (1994). BHT, BHA, EDTA and α-tocopherol
were used as reference antioxidant compounds. The new compounds were
examined in-vitro antimicrobial
properties against 6 different microorganisms (Bacillus substilis (ATCC11774), BacilluscCereus (ATCC11778),
Staphylococcus aureus (ATCC6538), Escherichia
coli (ATCC25922), Pseudomonas
aeruginosa (ATCC27853) and Klebsiella pneumonia (ATCC4352))
by the agar well method and the obtained results were evaluated. 

Anahtar Kelimeler

Mannich base, 4, 5-dihydro-1H-1, 2, 4-triazol-5-one, Antioxidant, Antimicrobial

Kaynakça

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