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Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro

Yıl 2018, Sayı: 3, 72 - 84, 01.12.2018

Öz

This study includes purification of
lipoxygenase from the rind of  pistacio
khinjuke by applying ammonium sulfate precipitation (70%), dialysis, ion
exchange chromatography, and the slab electrophoresis technique. One isoenzyme
has been obtained with a special activity of 1230x10-4 unit/mg protein and a molecular
weight of 101997 dalton. The partially purified rind lipoxygenase has been
characterized and inhibited by quercitine rutinoside and melatonine. The type
of inhibition seemed to be uncompetitive and the Ki values were 1.77 and 1.62
mM, respectively. Due to the importance of lipoxygenase for the animal, this
study also deals with the determination of its affinity toward quercitine
rutinoside and melatonine in the serum of normal and diabetic male mice. The
best doses for lipoxygenase inhibition were 523.3 and 8 Mg/Kg body weight of
quercitine rutinoside and melatonine, respectively. Treatment of animals with
these doses led to a significant decrease in lipoxygenase activity, glucose
level, and total cholesterol/high density lipoprotein cholesterol ratio in
animal serum. Therefore, these natural inhibitors seem to serve as a drug for
reducing the complications of some diseases (e.g. diabetes mellitus) which are
affected by this enzyme.

Kaynakça

  • Agar, I.T., Kaska, N. & Kafkas, S. (1995). Characterization of lipids in Pistacia species grown in Turkey. Acta Horticulturae, 419: 417–422. Axelrod, B., Pistoaus E.K. & Chistopher, J.P. (1972). Comparison of three lipoxygenases in the formation of linoleic acid hydroperoxide, 280 nm-absorbing product and coupled destruction of dye. Abstracts 11th World Congress, International Soc. for Fat. Babitha, M. P., Prakash H.S. & Shetty H. S. (2006). Induction of lipoxygenae in downy mildew resistantseedlings of pearl millet in response to inoculation with sclerospora graminicola. Int. J. Agri. Biol., 8(4): 560-564 . Befani, O., Grippa, E., Sasol., Turini, P. & Mondovi, B. (2001). Inhibition of monoamine oxidase by metronidazole, Inflamm. Res., 50 : 136-137. Birari, R.B. & Bhutani K..K.. (2007). Pancreatic lipase inhibitors from natural sources:Unexplored potential. Drug Discovery Today, 12:879-889. Boyes, S., Peyera, C. & Young, H. (1992). Kiwifruit lipoxygenase: preparation and characteristics. Journal of Food Science, 57(6):1390-1394. Calder, P.C. (2010). Omega – 3 fatty acids inflammatory processes, Nutrients, 2: 355-374 . Chang, J.J., Chen, T.H & Chen, Y.T. (2004). Inhibitory effect of tanui derivatives on HMG–CoA reductase in vitro cells. Pharmacol ., 62(4) : 224-228 . Chen, G., Huo, Y.,,Tan, D., Liang, Z., Zhang, W. & Zhang, Y. (2003). Melatonin in Chinese medicinal herbs. Life Sciences,73:19-26. Dellipizzi, A., Guan, H., Tong, X.,.Takizawa, H. & Nasjletti, A. (2000). Lipoxygenase–dependent mechanisms in hypertension. Clin. Exp.Hypertens. ,22:181-192. De-Man, F.H., Cabezas, M.C., Van-Barlingen, H.H., Erkelens, D.W. & Debruin, T.W (1996). Triglycerides rich lipoprotein sin non-insulin dependent diabetes mellitus post-prandial metabolism and relation to premature atherosclerosis. Eur. J . Clin . Invest ., 26 : 89-108 . Ding, X.Z., Hennig, R. and Adrian, T.E. (2003). Lipoxygenase and cyclooxygenase metabolism: new insights in treatment and chemoprevention of pancreatic cancer. Molecular Cancer, 2:10. Huo, Y., Zhao, L., Hyman, M. C., Shashkin, P., Harry, B. L., Burcin, T., Forlow, B., Stark, M. A., Smith, D. F., Clarke, S., Srinivasan, S., Hedrick, C.C., Pratico, D., Witztum, J. L., Nadler, J. L., Funk, C.D. & Ley K. (2004). Critical role of macrophage 12 /15-lipoxygenase for atherosclerosis in apolipoprotein E- deficient mice. Circulation , 110 : 2024-2031 . Hurt, G.B. & Axelrod, B. (1977). Characterization of two isoenzymes of lipoxygenase from bush beans. Plant Physiol. 59, 695-700. Imig, J.D. (2006). Eicosanoids and renal vascular function in diseases. Clin. Sci., 111: 21-34. Iranshahi, M., Askari, M., Sahebkar, A. & Hadjipavlou-Litina, D. (2009). Evaluation of antioxidant, anti-inflammatory and lipoxygenase inhibitory activities of the prenylated coumarin umbelliprenin. DARU , 17(2):99-103. Jankun, E.S., Mccabe, N.P., Selmans, H. & Jankun, UN. J. (2000). Curcumin inhibits lipoxygenase by binding to its central cavity: theoretical and X-ray evidence. InternationalJournal of Molecular Medicine, 6: 521-526. John, D.Y., Lee, G.J. & Lee, H.J. (1981). Rice bran lipoxygenase : Purification and characterization. Korean Biochem. J., 14(3): 243-252. Kumaraswamy, M.V. & Satish, S. (2008). Free radical scavenging activity and lipoxygenase inhibition of Woodfordia fructicosa Kurz and Betula utilis Wall. Afr. J. Biotech., 7 (12): 2013-2016. Malekian, F., Rao, R.M., Prinyawiwatkul, W., Marshall, W.E., Windhauser, M. & Ahmedna M. (2000). Lipase and lipoxygenase activity, functionality, and nutrient losses in rice bran during storage. Bulletin , 870: 1-69. Mckee, T. & Mckee, J.R. (2001). Biochemistry: an introduction. Boston: The McGraw-Hill Companies , Inc. Wm. C. Brown Pulishers, p:184 . Moosmann, B. & Behl, C. (2000). Cytoprotective antioxidant function of tyrosine and tryptophan residues in transmembrane proteins. European Journal of Biochemistry, 267: 5687-5692. Murphy, R. C. & Gljon M. A. (2007). Biosynthesis and metabolism of leukotrienes. Biochem. J., 405: 379-395. Murray, R.K., Bender, D.A., Botham, K..M., Kennelly, P.J., Rodwell, V.W. & Weil, P.A. (2009). Harper’s illustrated biochemistry. (28 th ed.), Boston: McGraw-Hill Companies, Inc. Natarajan, R. & Nadler, J. L. (2004). Lipid inflammatory mediators in diabetic vascular disease. Arterioscler. Thromb. Vasc. Biol., 24: 1542-1548. Nozawa, K., Tuch, M.L., Golub, M., Eggena, P., Nadler, J.L. & Stern, N. (1990). Inhibition of lipoxygenase pathway reduces blood pressure in renovascular hypertensive rats. Am.J. Physiol., 259: 1774-1780. Panda, H. (2004). Medicinal plants cultivation and their uses. New Delhi: National Institute of industrial research , p. 3 . Pikto-Pietkiewicz, W., Wolkowska, K., & Pasierski, T., (2005). Treatment of dysliprdemia in patients with diabetes mellitus. Pharmacological Reports, 57: 10-19. Pirbalouti A. G. and Aghaee K.(2011)."Chemical Composition of Essential Oil of Pistacia khinjuk Stocks Grown in Bakhtiari Zagross Mountains, Iran" . Electronic Journal of Biology, 7(4): 67-69. Rackova, L., Oblozinsky, M., Kostalova, D., Kettmann, V. & Bezakova, L. (2007). Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. Journal of Inflammation, 4( 1): 1-7. Robyt, F.J. & White J. B. (2001). Biochemical techniques ,theory and Practice. Calefornia: Brookes/Cole publishing company. Safari, M. & Alizadeh, H. (2007). Oil composition of Iranian major nuts. J. Agric. Sci. Technol., 9: 251-256. Scharcterle, G.R. & Pollack R.L. (1973). Simplified method quantitative assay for small amounts of proteins in biological materials. Anal. Biochem., 51: 645-655. Scribner, K..A., Gadbios, T.M., Gowri, M., Azhar, S. & Reaven, G.M. (2000). Masoprocol decreases serum triglyceride concentration in rats with fructose-induced hypertriglyceridemia. Metab. Clin.Exp.,49: 1106-1110. Sharkar, S., Pranava, M. & Marita, R. (1996). Demonstration of the hypoglycemic action Momordica charantia in a validated animals of diabetes. Pharmacol. Res., 33(1) : 1- 4 . Shastry, B.S. & Rao, M.R. (1975). Studies on lipoxygenase from rice bran. Cereal Chemistry, 52(5):597-603. Shibib, B.A., Khan, L.A. & Rahman, R. (1993). Hypoglycemic activity of Coccina indicia and Momordica charantia in diabetic rats : depression of the hepatic gluconeogenic enzymes (glu-6-ase) and (fruc-1,6-Dpas) and elevation of both liver and red cells shunt enzyme glucose-6-p dehydrogenase. Biochem .J., 15 : 267-270 . Yilmaz, E. (2001). Kinetic studies with crude tomato lipoxygenase. Turk. J. Agric. For., 25 : 291-296. Al-Araji, S. B. M. (2000). Isolation lipoxygenase enzyme and tripcine inhibitor from ibaa soybean and Purifying and describing them. Ph.D thesis: College of Agriculture, Baghdad University. Al-Flayyih, K. A. (2006). Introduction to biochemistry. (3rd ed.). Mosul: The University Press. Al-Juwari, H. M. S. (2009). Comparative diagnostic study of the surface and chemical characteristics of types of Pistacia vera L. in Nineveh Governorate.M.Sc. thesis: College of Agriculture, Mosul University. Al-Sultan, A. M. A., Al-Jumaili, T. K. H., & Al-Araji, S. B. M. (2004). Isolation, purification and Description of the lipoxygenase enzyme in field pistacia. Iraqi Journal of Agricultural Sciences, 35(5), 103-112. Muhaisi, I. K, Yahya, I. N., & Al-Araji, S. B. (2008). Description of the lipoxygenase enzyme isolated and purified from Arachis hpogaea L.seeds. Damascus Universiy Journal for Basic Sciences, 24(2).
Yıl 2018, Sayı: 3, 72 - 84, 01.12.2018

Öz

Kaynakça

  • Agar, I.T., Kaska, N. & Kafkas, S. (1995). Characterization of lipids in Pistacia species grown in Turkey. Acta Horticulturae, 419: 417–422. Axelrod, B., Pistoaus E.K. & Chistopher, J.P. (1972). Comparison of three lipoxygenases in the formation of linoleic acid hydroperoxide, 280 nm-absorbing product and coupled destruction of dye. Abstracts 11th World Congress, International Soc. for Fat. Babitha, M. P., Prakash H.S. & Shetty H. S. (2006). Induction of lipoxygenae in downy mildew resistantseedlings of pearl millet in response to inoculation with sclerospora graminicola. Int. J. Agri. Biol., 8(4): 560-564 . Befani, O., Grippa, E., Sasol., Turini, P. & Mondovi, B. (2001). Inhibition of monoamine oxidase by metronidazole, Inflamm. Res., 50 : 136-137. Birari, R.B. & Bhutani K..K.. (2007). Pancreatic lipase inhibitors from natural sources:Unexplored potential. Drug Discovery Today, 12:879-889. Boyes, S., Peyera, C. & Young, H. (1992). Kiwifruit lipoxygenase: preparation and characteristics. Journal of Food Science, 57(6):1390-1394. Calder, P.C. (2010). Omega – 3 fatty acids inflammatory processes, Nutrients, 2: 355-374 . Chang, J.J., Chen, T.H & Chen, Y.T. (2004). Inhibitory effect of tanui derivatives on HMG–CoA reductase in vitro cells. Pharmacol ., 62(4) : 224-228 . Chen, G., Huo, Y.,,Tan, D., Liang, Z., Zhang, W. & Zhang, Y. (2003). Melatonin in Chinese medicinal herbs. Life Sciences,73:19-26. Dellipizzi, A., Guan, H., Tong, X.,.Takizawa, H. & Nasjletti, A. (2000). Lipoxygenase–dependent mechanisms in hypertension. Clin. Exp.Hypertens. ,22:181-192. De-Man, F.H., Cabezas, M.C., Van-Barlingen, H.H., Erkelens, D.W. & Debruin, T.W (1996). Triglycerides rich lipoprotein sin non-insulin dependent diabetes mellitus post-prandial metabolism and relation to premature atherosclerosis. Eur. J . Clin . Invest ., 26 : 89-108 . Ding, X.Z., Hennig, R. and Adrian, T.E. (2003). Lipoxygenase and cyclooxygenase metabolism: new insights in treatment and chemoprevention of pancreatic cancer. Molecular Cancer, 2:10. Huo, Y., Zhao, L., Hyman, M. C., Shashkin, P., Harry, B. L., Burcin, T., Forlow, B., Stark, M. A., Smith, D. F., Clarke, S., Srinivasan, S., Hedrick, C.C., Pratico, D., Witztum, J. L., Nadler, J. L., Funk, C.D. & Ley K. (2004). Critical role of macrophage 12 /15-lipoxygenase for atherosclerosis in apolipoprotein E- deficient mice. Circulation , 110 : 2024-2031 . Hurt, G.B. & Axelrod, B. (1977). Characterization of two isoenzymes of lipoxygenase from bush beans. Plant Physiol. 59, 695-700. Imig, J.D. (2006). Eicosanoids and renal vascular function in diseases. Clin. Sci., 111: 21-34. Iranshahi, M., Askari, M., Sahebkar, A. & Hadjipavlou-Litina, D. (2009). Evaluation of antioxidant, anti-inflammatory and lipoxygenase inhibitory activities of the prenylated coumarin umbelliprenin. DARU , 17(2):99-103. Jankun, E.S., Mccabe, N.P., Selmans, H. & Jankun, UN. J. (2000). Curcumin inhibits lipoxygenase by binding to its central cavity: theoretical and X-ray evidence. InternationalJournal of Molecular Medicine, 6: 521-526. John, D.Y., Lee, G.J. & Lee, H.J. (1981). Rice bran lipoxygenase : Purification and characterization. Korean Biochem. J., 14(3): 243-252. Kumaraswamy, M.V. & Satish, S. (2008). Free radical scavenging activity and lipoxygenase inhibition of Woodfordia fructicosa Kurz and Betula utilis Wall. Afr. J. Biotech., 7 (12): 2013-2016. Malekian, F., Rao, R.M., Prinyawiwatkul, W., Marshall, W.E., Windhauser, M. & Ahmedna M. (2000). Lipase and lipoxygenase activity, functionality, and nutrient losses in rice bran during storage. Bulletin , 870: 1-69. Mckee, T. & Mckee, J.R. (2001). Biochemistry: an introduction. Boston: The McGraw-Hill Companies , Inc. Wm. C. Brown Pulishers, p:184 . Moosmann, B. & Behl, C. (2000). Cytoprotective antioxidant function of tyrosine and tryptophan residues in transmembrane proteins. European Journal of Biochemistry, 267: 5687-5692. Murphy, R. C. & Gljon M. A. (2007). Biosynthesis and metabolism of leukotrienes. Biochem. J., 405: 379-395. Murray, R.K., Bender, D.A., Botham, K..M., Kennelly, P.J., Rodwell, V.W. & Weil, P.A. (2009). Harper’s illustrated biochemistry. (28 th ed.), Boston: McGraw-Hill Companies, Inc. Natarajan, R. & Nadler, J. L. (2004). Lipid inflammatory mediators in diabetic vascular disease. Arterioscler. Thromb. Vasc. Biol., 24: 1542-1548. Nozawa, K., Tuch, M.L., Golub, M., Eggena, P., Nadler, J.L. & Stern, N. (1990). Inhibition of lipoxygenase pathway reduces blood pressure in renovascular hypertensive rats. Am.J. Physiol., 259: 1774-1780. Panda, H. (2004). Medicinal plants cultivation and their uses. New Delhi: National Institute of industrial research , p. 3 . Pikto-Pietkiewicz, W., Wolkowska, K., & Pasierski, T., (2005). Treatment of dysliprdemia in patients with diabetes mellitus. Pharmacological Reports, 57: 10-19. Pirbalouti A. G. and Aghaee K.(2011)."Chemical Composition of Essential Oil of Pistacia khinjuk Stocks Grown in Bakhtiari Zagross Mountains, Iran" . Electronic Journal of Biology, 7(4): 67-69. Rackova, L., Oblozinsky, M., Kostalova, D., Kettmann, V. & Bezakova, L. (2007). Free radical scavenging activity and lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. Journal of Inflammation, 4( 1): 1-7. Robyt, F.J. & White J. B. (2001). Biochemical techniques ,theory and Practice. Calefornia: Brookes/Cole publishing company. Safari, M. & Alizadeh, H. (2007). Oil composition of Iranian major nuts. J. Agric. Sci. Technol., 9: 251-256. Scharcterle, G.R. & Pollack R.L. (1973). Simplified method quantitative assay for small amounts of proteins in biological materials. Anal. Biochem., 51: 645-655. Scribner, K..A., Gadbios, T.M., Gowri, M., Azhar, S. & Reaven, G.M. (2000). Masoprocol decreases serum triglyceride concentration in rats with fructose-induced hypertriglyceridemia. Metab. Clin.Exp.,49: 1106-1110. Sharkar, S., Pranava, M. & Marita, R. (1996). Demonstration of the hypoglycemic action Momordica charantia in a validated animals of diabetes. Pharmacol. Res., 33(1) : 1- 4 . Shastry, B.S. & Rao, M.R. (1975). Studies on lipoxygenase from rice bran. Cereal Chemistry, 52(5):597-603. Shibib, B.A., Khan, L.A. & Rahman, R. (1993). Hypoglycemic activity of Coccina indicia and Momordica charantia in diabetic rats : depression of the hepatic gluconeogenic enzymes (glu-6-ase) and (fruc-1,6-Dpas) and elevation of both liver and red cells shunt enzyme glucose-6-p dehydrogenase. Biochem .J., 15 : 267-270 . Yilmaz, E. (2001). Kinetic studies with crude tomato lipoxygenase. Turk. J. Agric. For., 25 : 291-296. Al-Araji, S. B. M. (2000). Isolation lipoxygenase enzyme and tripcine inhibitor from ibaa soybean and Purifying and describing them. Ph.D thesis: College of Agriculture, Baghdad University. Al-Flayyih, K. A. (2006). Introduction to biochemistry. (3rd ed.). Mosul: The University Press. Al-Juwari, H. M. S. (2009). Comparative diagnostic study of the surface and chemical characteristics of types of Pistacia vera L. in Nineveh Governorate.M.Sc. thesis: College of Agriculture, Mosul University. Al-Sultan, A. M. A., Al-Jumaili, T. K. H., & Al-Araji, S. B. M. (2004). Isolation, purification and Description of the lipoxygenase enzyme in field pistacia. Iraqi Journal of Agricultural Sciences, 35(5), 103-112. Muhaisi, I. K, Yahya, I. N., & Al-Araji, S. B. (2008). Description of the lipoxygenase enzyme isolated and purified from Arachis hpogaea L.seeds. Damascus Universiy Journal for Basic Sciences, 24(2).
Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Ameera A. Al-juraısı

Omar Y. Al-abbasy

Yayımlanma Tarihi 1 Aralık 2018
Yayımlandığı Sayı Yıl 2018Sayı: 3

Kaynak Göster

APA Al-juraısı, A. A., & Al-abbasy, O. Y. (2018). Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro. The Eurasia Proceedings of Science Technology Engineering and Mathematics(3), 72-84.