Effect of Direct Electrical Current on Protoscoleces of Echinococcus Granulosus in vitro and on Immune Response of BALB/c Mice Against Infection with Secondary Hydatid Disease

Yıl 2018, Sayı: 3, 90 - 102, 01.12.2018

Asmaa Abdelazeez Alı Fouad Salım

Öz

The present study investigated the
effect of the direct electrical current on the viability of the protoscoleces
of Echinococcus granulosus in vitro using different voltages of electrical
current 3, 6, 9, 12 and 15, for different durations 3, 6, 9, 12 and 15 minutes,
and on the immune response in BALB/c mice against infection with secondary
hydatid cysts by injecting the mice with protoscoleces treated with direct
electrical currents with viability 75%, 50%, 25% and 0%, in comparison with the
control group, through six months, depending on many criteria included numbers,
weights and diameters of the hydatid cysts and the percentage of reduction of
their numbers, non-specific immune response represented by changes in the
phagocytic index and specific immune response represented by delayed type
hypersensitivity test (DTHT).The results revealed that the effect of direct
electrical current on protoscoleces viability in vitro increased with the
increase in voltage and exposure time. the reduction in the numbers of hydatid
cysts in treated mice was 100% , in groups injected with treated protoscoleces
with viability 75%, 50% and 25%, respectively. A significant increase
(p<0.01) in innate and cellular immune response in treated mice ,
represented by elevation in the rates of phagocytic index and delayed type hypersensitivity
(foot pad thickness).

Anahtar Kelimeler

Direct electrical current, Echinococcus granulosus, Hydatid disease, Cell-mediated immunity

Kaynakça

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