Protective Effects of Crocin on Experimental Gastrocnemius Muscle Ischemia/Reperfusion Model in Rat

Yıl 2018, Sayı: 3, 116 - 120, 01.12.2018

Betul Apaydın Yıldırım Selcuk Albayrak

Öz

The aim of the present study was to define whether
Crocin, has a regulatory effect againstexperimental gastrocnemius muscle
ischemia/reperfusion injury model in rats.A total of 32 Sprague-Dawley male,
200-250g rats were randomly divided into four groups: Control, Crocin
(60mg/kg/i.p), I/R and I/R+Crocin groups. All rats were anesthetized with
xylazine and ketamine. Except for the control and Crocin group, the left lower
limbs of the other 2 groups were applied to 2 hours of ischemia and 2 hours of
reperfusion with tourniquet. SOD, MDA, GSH, CAT, GPx, MPO, NO, IMA, AOPP ve PON
levels were measured in the plasma; the levels of SOD, MDA, GSH, CAT and GPx
were determined in gastrocnemius muscle. Increases in SOD, GSH, CAT, GPx, AOPP
and PON levels, decreases in the MDA, MPO, NO and IMA levels were significant
in the Crocin groups according to the I/R group. These differences were
statistically significant. The results of this study support the possibility
that Crocin may play a protective role against skeletal muscle injury caused by
I/R in rats by reducing oxidative stress. Crocin can be safely used for
ischemia/reperfusion injury.  

Anahtar Kelimeler

Crocin, Ischemia, Rat, Oxidative stress, Reperfusion

Kaynakça

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