Araştırma Makalesi
BibTex RIS Kaynak Göster

Molecular Survey of Hepatozoon canis in Dogs from Samsun Province of Northern Part of Turkey

Yıl 2016, Cilt: 27 Sayı: 2, 104 - 107, 15.12.2016
https://doi.org/10.35864/evmd.514510

Öz

We attempt to address new information of haematozoan infections in dogs from Samsun province of Turkey. The diagnostic techniques using genus specific polymerase chain reaction designed to amplify a fragment of ∼666 bp located in 18S ribosomal RNA (rRNA) gene of Hepatozoon spp. include microscopic investigations. H. canis identities were confirmed by sequencing the 18S rRNA gene. Two hundred (170 stray and 30 owned dogs) blood samples from asymptomatic dogs treated for endo and ecto parasites were examined. There were no detected ticks on dogs in the clinical examinations. None of 200 thin blood smears were positive for the presence of Hepatozoon spp. gametocytes. Only one dog sample from 200 with a prevalence of 0.5% was found to be positive for the presence of Hepatozoon DNA demonstrates a chronic infection. Partial sequences of the 18S rRNA gene shared 99-100% similarity with the corresponding H. canis isolates. Contrary the other molecular surveys on H. canis among Turkey, this research revealed a very low prevalence of H. canis in dogs from Samsun province of Turkey, and it indicates that ectoparasite control programs have a great impact on decreasing the vector borne parasitic diseases especially in the stray and owned dogs.

Kaynakça

  • 1. Aktas M, Ozubek S, Altay K, Balkaya I, Utuk AE, Kırbas A, Simsek S, Dumanlı N, (2015). A molecular and parasitological survey of Hepatozoon canis in domestic dogs in Turkey. Vet Parasitol. 209(3), 264-267.
  • 2. Aktas M, Ozubek S, Ipek DNS, (2013). Molecular investigations of Hepatozoon species in dogs and developmental stages of Rhipicephalus sanguineus. Parasitol Res. 112(6), 2381-2385.
  • 3 Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, (1990). Basic local alignment search tool. J Mol Biol. 215(3), 403-410.
  • 4. Aydin MF, Sevinc F, Sevinc M, (2015). Molecular detection and characterization of Hepatozoon spp. in dogs from the Central part of Turkey. Ticks and Tick-borne Diseases. 6(3), 388-392.
  • 5. Baneth G, (2011). Perspectives on canine and feline hepatozoonosis. Vet Parasitol. 181(1), 3-11.
  • 6. Baneth G, Bourdeau P, Bourdoiseau G, Bowman D, Breitschwerdt E, Capelli G, Cardoso L, Dantas-Torres F, Day M, Dedet JP, Dobler G, Ferrer L, Irwin P, Kempf V, Kohn B, Lappin M, Little S, Maggi R, Miro G, Naucke T, Oliva G, Otranto D, Penzhorn B, Pfeffer M, Roura X, Sainz A, Shaw S, Shin S, Solano-Gallego L, Straubinger R, Traub R, Trees A, Truyen U, Demonceau T, Fitzgerald R, Gatti D, Hostetler J, Kilmer B, Krieger K, Mencke N, Mendao C, Mottier L, Pachnicke S, Rees B, Siebert S, Stanneck D, Tarancon Mingote M, von Simson C, Weston S, (2012). Vector-Borne Diseasesconstant challenge for practicing veterinarians: recommendations from the CVBD World Forum. Parasites & Vectors, 5, 55-58.
  • 7. Baneth G, Vincent-Johnson N, (2005). Hepatozoonosis. Shaw SE, Day MJ. eds. Arthropod-borne Infectious Diseases of the Dog and Cat. Manson Publishing, London. p. 78-89.
  • 8. Düzlü Ö, İnci A, Yıldırım A, Önder Z, Çiloğlu A, (2014). Köpeklerde kene kaynaklı bazı protozoon ve rickettsial enfeksiyonların real time PCR ile araştırılması ve saptanan izolatların moleküler karakterizasyonları. Ankara Univ Vet Fak Derg. 61, 275-282.
  • 9. Inokuma H, Okuda M, Ohno K, Shimoda K, Onishi T, (2002). Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Vet Parasitol, 106, 265-271.
  • 10. Karagenc TI, Pasa S, Kirli G, Hosgor M, Bilgic HB, Ozon YH, Atasoy A, Eren H, (2006). A parasitological, molecular and serological survey of Hepatozoon canis infection in dogs around the Aegean coast of Turkey. Vet Parasitol. 135(2), 113-119.
  • 11. Otranto D, Dantas-Torres F, Weigl S, Latrofa MS, Stanneck D, Decaprariis D, Baneth G, (2011). Diagnosis of Hepatozoon canis in young dogs by cytology and PCR. Parasites & Vectors, 4(1), 55.
  • 12. Smith TG, (1996). The genus Hepatozoon (Apicomplexa: Adeleina). J Parasitol. 82, 565-585.
  • 13. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 28(10), 2731-2739.
  • 14. Thompson JD, Higgins DG, Gibson TJ, (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22(22), 4673-4680.
  • 15. Tuzdil AN, (1933). Bizde ilk defa görülen bir Hepatozoon canis vakası. Türk Bay Cem Mec. 13, 35.
  • 16. Voyvoda H, Pasa S, Uner A, (2004). Clinical Hepatozoon canis infection in a dog in Turkey. J Small Anim Pract. 45(12), 613-617.
Toplam 16 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Tam Sayı
Yazarlar

Cenk Soner Bölükbaş

Didem Pekmezci Bu kişi benim

Ali Tümay Gürler Bu kişi benim

Gökmen Zafer Pekmezci Bu kişi benim

Murat Güzel Bu kişi benim

Mustafa Açıcı Bu kişi benim

Şinasi Umur Bu kişi benim

Yayımlanma Tarihi 15 Aralık 2016
Gönderilme Tarihi 6 Ekim 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 27 Sayı: 2

Kaynak Göster

APA Bölükbaş, C. S., Pekmezci, D., Gürler, A. T., Pekmezci, G. Z., vd. (2016). Molecular Survey of Hepatozoon canis in Dogs from Samsun Province of Northern Part of Turkey. Etlik Veteriner Mikrobiyoloji Dergisi, 27(2), 104-107. https://doi.org/10.35864/evmd.514510


15430