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Co-infection with Border Disease Virus and Brucella melitensis in an Aborted Sheep Foetus

Yıl 2017, Cilt: 28 Sayı: 2, 65 - 68, 15.12.2017
https://doi.org/10.35864/evmd.516685

Öz

In this study, we investigated the potential roles of BDV and Brucella melitensis infections in a case of sheep abortion. Internal organ specimens from aborted sheep foetus and EDTA whole blood sample from mother of the foetus were collected from a sheep flock in the Konya Province in the Central Anatolia region of Turkey in 2017. The presence of Brucella spp. directly assessed by bacterial isolation and detection of BDV was carried by real time RT-PCR. Genetic characterization of the BDV field isolate was conducted by sequencing the 5’- end untranslated region (UTR) region of BDV. Brucella strain was isolated from the samples of aborted sheep foetus, and it was identified as Brucella melitensis by biochemical characteristics, agglutination with monospecific A and M sera. BDV RNA was detected in EDTA whole blood sample and aborted sheep foetus. Phylogenetic analysis in 5’-UTR region allocated the field isolate of BDV obtained in this study into BDV-7 genotype. To the best of our knowledge, this is the first report on the dual infection of aborted sheep foetus with BDV and Brucella melitensis.

Kaynakça

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  • 3. Díaz Aparicio E, (2013). Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Tech. 32, 43-51, 53-60.
  • 4. European Commission, Health & Consumers Directorate- General (2012). Eradication programme for Sheep and Goat Brucellosis (B. Melitensis). Erişim adresi: https:// ec.europa.eu/food/sites/food/files/safety/docs/cff_animal_ vet-progs_2012_dec-2011-807-ec_ov-cap-brucellosis_grc. pdf, Erişim tarihi: 08.08.2017
  • 5. Hawari AD, (2012). Epidemiological Studies, Seroprevalance and Some Risk Factors of Brucellosis in Sheep and Goats in the South Province of West Bank. Asian J Anim Vet Adv. 7, 535-539.
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  • 11. Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ, (1994). Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol. 136, 309-323.
  • 12. Office International des Epizooties (OIE), (2017). Border Disease. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. OIE, Paris, 1-13. Available at: http:// www.oie.int/fileadmin/Home/eng/Health_standards/ tahm/2.07.01_BORDER_DIS.pdf (accessed on 25 August 2017).
Toplam 12 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Tam Sayı
Yazarlar

Murat Şevik Bu kişi benim

Yasin Gülcü Bu kişi benim

Müge Doğan Bu kişi benim

Yayımlanma Tarihi 15 Aralık 2017
Gönderilme Tarihi 18 Eylül 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 28 Sayı: 2

Kaynak Göster

APA Şevik, M., Gülcü, Y., & Doğan, M. (2017). Co-infection with Border Disease Virus and Brucella melitensis in an Aborted Sheep Foetus. Etlik Veteriner Mikrobiyoloji Dergisi, 28(2), 65-68. https://doi.org/10.35864/evmd.516685


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