Bilirubin Damgalı Kitosan-Agaroz Biyomateryalinin Sentezi, Karakterizasyonu ve Bilirubin Uzaklaştırılmasında Kullanımı

Yıl 2018, Sayı: 5, 451 - 463, 31.08.2018

Arzu Kaya Koçdoğan Sema Çetin

Öz

Amaç: Bilirubin
damgalı kitosan-agaroz biyomateryalinin; sentezi, karakterizasyonu ve bilirubin
uzaklaştırılmasında kullanımının önemini tespit etmek amacıyla yapılmıştır.

Yöntem: Kitosan ve agaroz doğal polimerleri materyal olarak seçilmiştir. Bilirubin
damgalı kitosan-agaroz biyomateryalinin bilirubin uzaklaştırma kapasitesinin
belirlenmesi için insan plazması kullanılmıştır.

Bulgular: Bilirubin
uzaklaştırılması için pH 7.0, sıcaklık 37^oC ve başlangıç bilirubin
konsantrasyonu 40 mg/g optimum şartlar olarak belirlenmiştir. NaCl
konsantrasyonunun arttırılması ile bilirubin için iyonik şiddetin azaldığı
tespit edilmiştir.

Sonuç: Etkileşim
öncesi ve sonrasında alınan serum örneklerinde bilirubin analizi
gerçekleştirildi ve moleküler damgalı kitosan-agaroz biyomateryalleri ile
%87’lik bir uzaklaştırma oranı elde edildi. Sulu çözeltilerden bilirubin
adsorpsiyonu ile insan plazmasından bilirubin adsorpsiyonu kıyaslandığında,
plazmada adsorpsiyon kapasitesinin çok az miktarda azaldığı gözlendi.

Anahtar Kelimeler

bilirubin, kitosan-agaroz, Moleküler damga, biyomateryaller

Bilirubin Impriting Chitosan-Agarose Biomaterial Synthesis, Characterization and Use to Bilirubin Removal

Öz

Aim: This study was conducted to synthesize and
characterize bilirubin imprinted chitosan-agarose biomaterial and to determine
its significance on bilirubin removal.

Method: Natural Chitosan and agarose polymers were chosen as materials. Human
plasma was used to determine the capacity of bilirubin imprinted
chitosan-agarose biomaterial for bilirubin removal.

Findings: The optimum
conditions for bilirubin removal were determined as pH 7.0 at 37^oC
with a starting concentration of 40 mg/g. An increase in NaCl concentration was
shown to decrease the ionic strength for bilirubin. 

Conclusion: Bilirubin analysis was performed on serum
samples taken before and after the interaction. A suspension rate of 87%  was obtained by using molecular imprinted
chitosan-agarose biomaterials. When bilirubin adsorption from aqueous solutions
was compared to the adsorption of bilirubin from human plasma it was observed
that the adsorption capacity of plasma was slightly decreased.

Anahtar Kelimeler

Molecular imprinting, bilirubin, agarose-chitosan, biomaterials

Kaynakça

• Deng J, Nian Y, Syu M. Investigation of binding specificity of PMAA toward α-bilirubin. In: The 1st imprinted International Meeting on Microsensors and Microsystems; January, 12-14, 2003; National Cheng Kung University, Tainan, Taiwan.
• Syu MJ, Deng JH, Nian YM, Chiu TC, Wu AH. Chiu binding specificity of alpha-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward alpha-bilirubin. Biomaterials. 2005;26(22):4684-4692.
• Kwok AY, Qiao GG, Solomon DH. Synthetic hydrogels 3. Solvent effects on poly (2-hydroxyethyl methacrylate) networks. Polymer. 2004;45(12):4017-4027.
• Ma Q, Wooley KL. The preparation of t-butyl acrylate, methyl acrylate, and styrene block copolymers by atom transfer radical polymerization: Precursors to amphiphilic and hydrophilic block copolymers and conversion to complex nanostructured materials. Polymer Chemistry. 2000;38(S1):4805-4820. doi: 10.1002/1099-0518(200012)38:1+<4805::AID-POLA180>3.0.CO;2-O.
• Asayama S, Kawakami H, Nagaoka S. Design of a poly (L-histidine)-carbohydrate conjugate for a new pH-sensitive drug carrier. Polymers for Advanced Technologies. 2004;15(8):439–444. doi: 10.1002/pat.493.
• Yang Z, Si S, Fung Y. Bilirubin adsorption on nanocrystalline titania films. Thin Solid Films. 2007;515(7-8):3344–3351. doi: http://dx.doi.org/10.1016/j.tsf.2006.09.018.
• Yurdakök M. Hiperbilirubinemide ışık ve ilaç tedavisi. Katkı Pediatri Dergisi. 1995;(5):725-733.
• Trotta F, Baggiani C, Luda MP, Drioli E, Massari T. A molecular imprinted membrane for molecular discrimination of tetracycline hydrochloride. Journal of Membrane Science. 2005;254(1–2):13-19. doi: https://doi.org/10.1016/j.memsci.2004.11.013.
• Baydemir G, Bereli N, Andaç M, Say R, Galaev IY, Denizli A. Bilirubin recognition via molecularly imprinted supermacroporous cryogels. Colloids and Surfaces B: Biointerfaces. 2009;68(1):33-38.
• Denizli A, Kocakulak M, Pişkin E. Specific sorbents for bilirubin removal from human plasma: Congo red-modified poly (EGDMA/HEMA) microbeads. Applied Polymer Science. 1998;68(3):373–380.
• Demirci G, Doğaç Yİ, Teke M. A selective molecularly imprinted polymer for immobilization of acetylcholinesterase (AChE): An active enzyme targeted and efficient method. J Mol Recognit. 2015;28(11):645-650. doi: 10.1002/jmr.2475.
• Derazshamshir A, Baydemir G, Yılmaz F, Bereli N, Denizli A. Preparation of cryogel columns for depletion of hemoglobin from human blood. Artificial Cells, Nanomedicine and Biotechnology. 2016;44(3):792-799. doi: 10.3109/21691401.2015.1129623.
• Yavuz H, Say R, Denizli A. Iron removal from human plasma based on molecular recognition using imprinted beads. Materials Science and Engineering: C. 2005;25(4):521-528. doi: https://doi.org/10.1016/j.msec.2005.04.005.
• McClure CD, Schiller NL. Effects of Pseudomonas aeruginosa rhamnolipids on human monocyte-derived macrophages. Journal of Leukocyte Biology. 1992;51(2):97-102.