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ULTRASTRUCTURAL EXAMINATIONS ON DIABETIC RAT SKIN TISSUE WITH TOPICAL APPLICATION OF Salvia euphratica ETHANOL EXTRACT

Yıl 2018, Cilt: 3 Sayı: 3, 107 - 116, 26.12.2018
https://doi.org/10.23884/ijhsrp.2018.3.3.02

Öz



Several medicinal plants to treat diabetic wound
healing were researched and are still under investigation. A variety of processes
contributed to diabetics such as;
inhibition of inflammatory response, generation of reactive oxygen species, differentiation of the extracellular matrix
and reduced
collagen production. The purpose of this study was to examine the ultrastructural
alterations in diabetic rat skin tissue treated with the ointment prepared with
Salvia euphratica. Male Wistar albino rats were used in
this study (n: 24), divided into 4 groups. Non-treated, diabetic, diabetic and
cicatrizant  treated, ointment prepared
with ethanol extract of   1% Salvia
euphratica  topically applied  for  14
days. A single dose of 45 mg/dL streptozotocin (i.p.) was given to rats to
induce diabetes. Excisional wound model was created under anesthesia. A cicatrizant,
fitocream, was used as positive control. Normal skin tissue was observed in
non-treated group. Diabetic skin tissue  
revealed hyalinization of the cytoplasm and loss of cytoplasm.    Ointment treated diabetic group revealed
altered cellular elements suggests healing. At the wound area, number of
fibroblast cells synthesizing connective tissue were increased and the collagen
fibers were regularly oriented. Neutrophil and monocyte cells besides mast and
macrophage cells were found in the capillaries of dermis in S. euphratica
ointment treated group.
Wound healing in diabetic tissues is known to
be slow. Using phytocream therapy to expedite the healing process is a long
time known phenomenon. Our observations demonstrated that Salvia euphratica
promoted changes in skin tissue that may contribute to wound healing at the
cellular level.




Kaynakça

  • Referans1 Tsioutsiou, E., Miraldi, E., et al., Skin Wound Healing: From Mediterranean Ethnobotany to Evidence based Phytotherapy, Athens Journal of Sciences, (2017), 4, pp. 199-211.
  • Referans2 Bandaranayake, W. M., Quality control, screening, toxicity, and regulation of herbal drugs, Modern Phytomedicine. Turning Medicinal Plants into Drugs eds Ahmad I., Aqil F., Owais M., John Wiley and Sons Inc., New York, USA, pp. 25–57.
  • Referans3 Bamidele, O., Kolawole, J.T., et al., Wound Healing Potentials of Aqueous Leaf Extract of Mangifera indica L. in Wistar Rats. Journal of Complementary and Alternative Medical Research 2 (2017), pp. 1-11.
  • Referans4 Dorai, A.A., Wound care with traditional, complementary and alternative medicine. Indian Journal of Plastic Surgery, 45 (2012), pp. 418-424.
  • Referans5 Nagar, H.K., Srivastava, A.K, et al. Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats. Journal of Pharmaceutics, (2016), ID 9249040.
  • Referans6 Nguyen, V.L, Truong, C-T., et al. Anti-inflammatory and wound healing activities of calophyllolide isolated from Calophyllum inophyllum Linn. PLoS ONE. (2017), 12(10):e0185674.
  • Referans7 Greenhalgh, D.G. Wound healing and diabetes mellitus. Clinical Plastic Surgery, 30 (2003), pp. 37 – 45.
  • Referans8 Trengove, N.J, Stacey, MC, et al. Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors. Wound Repair Regeneration, (1999), 7, pp. 442– 452.
  • Referans9 Alimpic-Aradski, Knezevi, A., et al., Biological activities and chemical composition of Salvia amplexicaulis Lam. Extracts. Industrial crops and products, 105, (2017), 1-9.
  • Referans10 Narayan, S., Sasmal, D., et al., Evaluation Of The Wound Healing Effect Of Herbal Ointment Formulated with Salvia Splendens (Scarlet Sage) International Journal of Pharmarcy Science, 3, (2011), 195-199.
  • Referans11 Estakhr, J. and Javdan, N., Evaluation of Wound Healing Activity of Salvia hypoleuca extract on Rats. Pharmacologyonline (2011), 3, pp.622-625.
  • Referans12 Suntar, I., Akkol, E.K., et al. Investigating wound healing, tyrosinase inhibitory and antioxidant activities of the ethanol extracts of Salvia cryptantha and Salvia cyanescens using in vivo and in vitro experimental models. Journal of Ethnopharmacology, (2011), 135, pp.71-77.
  • Referans13 Hayat, M.A., Principles and Techniques of Electron Microscopy, Biological Applications. Cambridge University Press, Cambridge, UK, 2000.
  • Referans14 Frangez, I., Cankar, K., Frangez, H.B., Smrke, D.M., The effect of LED on blood microcirculation during chronic wound healing in diabetic and non-diabetic patients-a prospective, double-blind randomized study. Lasers in Medical Science, 32, (2017), pp.887-894.
  • Referans15 Medagama, A.B, Bandara, R., The use of complementary and alternative medicines (CAMs) in the treatment of diabetes mellitus: is continued use safe and effective? Nutrition Journal, 13, (2014), pp.1-9.Referans16 Komesu, M.C., Tanga, M.B., Buttros, K.R., Nakao, C., Effects of acute diabetes on rat cutaneous wound healing. Pathophysiology, (2004), pp.63-67.
  • Referans17 Geddes, A.M., Pithie, A.D., Infection in the diabetic patient, in: M. Brownlee, L.M. Sherwood (Eds.), Diabetes Mellitus and Its Complications: Pathogenesis and Treatment, Hanly & Belfus Inc., Philadelphia, 1990.
Yıl 2018, Cilt: 3 Sayı: 3, 107 - 116, 26.12.2018
https://doi.org/10.23884/ijhsrp.2018.3.3.02

Öz

Kaynakça

  • Referans1 Tsioutsiou, E., Miraldi, E., et al., Skin Wound Healing: From Mediterranean Ethnobotany to Evidence based Phytotherapy, Athens Journal of Sciences, (2017), 4, pp. 199-211.
  • Referans2 Bandaranayake, W. M., Quality control, screening, toxicity, and regulation of herbal drugs, Modern Phytomedicine. Turning Medicinal Plants into Drugs eds Ahmad I., Aqil F., Owais M., John Wiley and Sons Inc., New York, USA, pp. 25–57.
  • Referans3 Bamidele, O., Kolawole, J.T., et al., Wound Healing Potentials of Aqueous Leaf Extract of Mangifera indica L. in Wistar Rats. Journal of Complementary and Alternative Medical Research 2 (2017), pp. 1-11.
  • Referans4 Dorai, A.A., Wound care with traditional, complementary and alternative medicine. Indian Journal of Plastic Surgery, 45 (2012), pp. 418-424.
  • Referans5 Nagar, H.K., Srivastava, A.K, et al. Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats. Journal of Pharmaceutics, (2016), ID 9249040.
  • Referans6 Nguyen, V.L, Truong, C-T., et al. Anti-inflammatory and wound healing activities of calophyllolide isolated from Calophyllum inophyllum Linn. PLoS ONE. (2017), 12(10):e0185674.
  • Referans7 Greenhalgh, D.G. Wound healing and diabetes mellitus. Clinical Plastic Surgery, 30 (2003), pp. 37 – 45.
  • Referans8 Trengove, N.J, Stacey, MC, et al. Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors. Wound Repair Regeneration, (1999), 7, pp. 442– 452.
  • Referans9 Alimpic-Aradski, Knezevi, A., et al., Biological activities and chemical composition of Salvia amplexicaulis Lam. Extracts. Industrial crops and products, 105, (2017), 1-9.
  • Referans10 Narayan, S., Sasmal, D., et al., Evaluation Of The Wound Healing Effect Of Herbal Ointment Formulated with Salvia Splendens (Scarlet Sage) International Journal of Pharmarcy Science, 3, (2011), 195-199.
  • Referans11 Estakhr, J. and Javdan, N., Evaluation of Wound Healing Activity of Salvia hypoleuca extract on Rats. Pharmacologyonline (2011), 3, pp.622-625.
  • Referans12 Suntar, I., Akkol, E.K., et al. Investigating wound healing, tyrosinase inhibitory and antioxidant activities of the ethanol extracts of Salvia cryptantha and Salvia cyanescens using in vivo and in vitro experimental models. Journal of Ethnopharmacology, (2011), 135, pp.71-77.
  • Referans13 Hayat, M.A., Principles and Techniques of Electron Microscopy, Biological Applications. Cambridge University Press, Cambridge, UK, 2000.
  • Referans14 Frangez, I., Cankar, K., Frangez, H.B., Smrke, D.M., The effect of LED on blood microcirculation during chronic wound healing in diabetic and non-diabetic patients-a prospective, double-blind randomized study. Lasers in Medical Science, 32, (2017), pp.887-894.
  • Referans15 Medagama, A.B, Bandara, R., The use of complementary and alternative medicines (CAMs) in the treatment of diabetes mellitus: is continued use safe and effective? Nutrition Journal, 13, (2014), pp.1-9.Referans16 Komesu, M.C., Tanga, M.B., Buttros, K.R., Nakao, C., Effects of acute diabetes on rat cutaneous wound healing. Pathophysiology, (2004), pp.63-67.
  • Referans17 Geddes, A.M., Pithie, A.D., Infection in the diabetic patient, in: M. Brownlee, L.M. Sherwood (Eds.), Diabetes Mellitus and Its Complications: Pathogenesis and Treatment, Hanly & Belfus Inc., Philadelphia, 1990.
Toplam 16 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Article
Yazarlar

Ebru Gokalp-ozkorkmaz

Nursel Gul

Yusuf Ozay Bu kişi benim

Sevda Guzel Bu kişi benim

Ahmet Kahraman Bu kişi benim

Hakan Eskızengın

Yayımlanma Tarihi 26 Aralık 2018
Gönderilme Tarihi 30 Ekim 2018
Kabul Tarihi 2 Aralık 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 3 Sayı: 3

Kaynak Göster

IEEE E. Gokalp-ozkorkmaz, N. Gul, Y. Ozay, S. Guzel, A. Kahraman, ve H. Eskızengın, “ULTRASTRUCTURAL EXAMINATIONS ON DIABETIC RAT SKIN TISSUE WITH TOPICAL APPLICATION OF Salvia euphratica ETHANOL EXTRACT”, IJHSRP, c. 3, sy. 3, ss. 107–116, 2018, doi: 10.23884/ijhsrp.2018.3.3.02.

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