BibTex RIS Kaynak Göster

Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle

Yıl 2015, Cilt: 41 Sayı: 2, 218 - 222, 01.07.2015

Öz

Fat composition in ruminant’s milk is one of the factors that can affect human health in positive or adverse ways. Optimizing ruminant feed to achieve ideal fatty acid composition in milk has been an ongoing area of research in recent years, without satisfactory results to date. It has been argued that in addition to changes in feed, genetic information can also be utilized to improve milk fatty acid composition. The aim of the study is to investigate the incidence of stearoyl-CoA-desaturase 1 (SCD) gene variants, which are claimed to affect fat content and quality of milk in Turkish native cattle breeds. Fifty South Anatolian Red (SAR) and 50 East Anatolian Red (EAR) cattle were used in the study.  The 5th exon of SCD gene was amplified using polymerase chain reaction (PCR) and the PCR products were subjected to sequencing analysis. Among the samples sequenced polymorphism at three nucleotide positions have been observed on the 5th exon of the SCD gene, namely A702G, T762C and C878T. Of these three, the polymorphic position C878T was utilized to determine peptide variants of A (293Ala) or the V (293 Val) of individual samples. Frequency of A variant and AA genotype in SAR and EAR cattle breeds was 0.91 and 0.77 as well as 0.43 and 0.29, respectively. In particular the SAR exhibits a very low frequency of the V allele, believed to have been an ancestral allele. In both samples, 2 individuals were identified to have the VV genotype. The results suggested that high frequency of A allele and AA genotype which confers great advantage on milk composition and meat fatty acid composition was present in SAR and EAR cattle breeds

Kaynakça

  • Akıs, A., Oztabak, K., 2013. Bos indicus associated alleles in Anatolian cattle breeds support zebu introgression into Near East. Journal of Biological Research-Thessaloniki 19, 131-138.
  • Corl, B.A., Baumgard, L.H., Dwyer, D.A., Griinari, J.M., Phillips, B.S., Bauman, D.E., 2001. The role of Δ9-desaturase in the production of cis-9, trans-11 CLA. Journal of Nutritional Biochemistry 12, 622-630.
  • Edwards, C.J., Baird, J.F., Machugh, D.E., 2007. Taurine and Zebu admixture in Near Eastern cattle: A comparison of mitochondrial, autosomal and Y-chromosomal data. Animal Genetics 38, 520-524.
  • Grummer, R.R., 1991. Effect of feed on composition of milk fat. Journal of Dairy Science 74, 3244-3257.
  • Islas-Trejo, A., Fragment Johnson, A., Medrano, F.J., 2002. Genomic structure and expression of bovine stearoyl- CoA desaturase gene. In: Proceeding of Plant, Animal and Microbe Genomes Conference, San Diego, CA., 766.
  • Kgwatalala, P.M., Ibeagha-Awemu, E.M., Mustafa, A.F., Zhao, X., 2009. Influence of stearoyl-coenzyme A desaturase 1 genotype and stage of lactation on fatty acid composition of Canadian Jersey cows. Journal of Dairy Science 92, 1220-1228.
  • Kromhout, D., Menotti, A., Kestleloot, H., Sans, S., 2002. Prevention of coronary heart disease by diet and lifestyle: Evidence from prospective cross-cultural, cohort and interventional studies. Circulation 105, 893- 898.
  • Macciota, N.P.P., Mele, M., Conte, G., Serra, A., Cassandro, M., Dal Zotto, R., Borlino, A.C., Pagnacco, G., Secchiari, P., 2008. Association between an polymorphism at the stearoyl CoA desaturase locus and milk production traits in Italian Holsteins. Journal of Dairy Science 91, 3184-3189.
  • Matsuhashi, T., Maruyama, S., Uemoto, Y., Kobayashi, N., Mannen, H., Abe T., Sakaguchi, S., Kobayashi, E., 2011. Effects of bovine fatty acid synthase, stearoyl- coenzyme A desaturase, sterol regulatory element- binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle. Journal of Dairy Science 89, 12-22.
  • Medrano, J.F., Johnson, A., Depeters, E.J., Islas A., 1999. Genetic modification of composition of milk fat: Identification of polymorphisms within the bovine stearoyl-CoA- desaturase gene. Journal of Dairy Science 82, 71-75.
  • Mele, M., Conte, G., Castiglioni, B., Chessa, S., Macciotta, N.P.P., Sera, A., Buccioni, A., Pagnacco, G., Secchiari, P., 2007. Stearoyl-coenzyme A desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. Journal of Dairy Science 90, 4458- 4465.
  • Milanesi, E., Nicoloso, L., Crepaldi, P., 2008. Stearoyl CoA desaturase (SCD) gene polymorphisms in Italian cattle breeds. Journal of Animal Breeding and Genetics 125, 63-67.
  • Miller, M., Dykes, D.D., Polesky, H.F., 1985. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16, 1215-1219.
  • Moioli, B., Contarini, G., Avalli, A., Catillo, G., Orru, L., De Matteis, G., Masoero, G., Napolitano, F., 2007. Effect of stearoyl-coenzyme A desaturase polymorphism on fatty acid composition of milk. Journal of Dairy Science 90, 3553-3558.
  • Ohsaki, H., Tanaka, A., Hoashi, S., Sasazaki, S., Oyama, K., Taniguchi, M., Mukai, F., Mannen, H., 2009. Effects of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herd. Animal Science Journal 80, 225-232.
  • Schennink, A., Heck, J.M.L., Bovenhuis, H., Visker M.H.P.W., Van Valenberg, H.J.F., Van Aredonk, J.A.M., 2008. Milk fatty acid unsaturation : genetic parameters and effects of stearoyl-coenzyme A desaturase (SCD1) and acyl Co A: Diacylglycerol acyltransferase (DGAT1). Journal of Dairy Science 91, 2135-2143.
  • Taniguchi, M., Utsugi, T., Oyama, K., Manen, H., Kobayashi, M., Tanabe, Y., Ogino, A., Tsuji, S., 2004. Genotype of stearoyl-coenzyme A desaturase is associated with fatty acid composition in Japanese Black cattle. Mammalian Genome 14, 142-148.
  • Yeh, F., Yang, R.C., Boyle, T., 2000. Popgene (v.1.32) Microsoft Windows-based freeware for Population Genetic Analysis. http://www.ualberta.ca/~fyeh/ Pop32.exe. (Erişim 01.10.2011). from

Güney Anadolu Kırmızısı ve Doğu Anadolu Kırmızısı Irkı Sığırlarda Stearoil-Koenzim A Desatüraz 1 Geni Varyantlarının Belirlenmesi

Yıl 2015, Cilt: 41 Sayı: 2, 218 - 222, 01.07.2015

Öz

Ruminantlarda sütteki yağ içeriği insan sağlığını pozitif veya negatif olarak etkileyen faktörlerdendir. Süt yağ asidi içeriğinin en ideal şekle kavuşturulması amacıyla ruminantların yem içeriğinin değiştirilmesi üzerine son yıllarda çalışmalar yapılmış olmasına karşın tatmin edici sonuçlar elde edilememiştir. Süt yağ asidi kompozisyonunun geliştirilmesinde yem değişikliklerinin yanı sıra genetik bilginin de kullanılmasının yaralı olabileceği ileri sürülmüştür. Çalışmamızda Güney Anadolu Kırmızısı (GAK) ve Doğu Anadolu Kırmızısı (DAK) ırkı sığırlarda süt yağ içeriğini ve kalitesini etkilediği ileri sürülen Stearoil-KoA-desatüraz 1 (SCD1) geni varyantlarının dağılımının belirlenmesi amaçlanmıştır. Bu amaçla çalışmada 50’şer baş GAK ve DAK ırkı sığır kullanılmıştır. Genomik DNA örneklerinde SCD1 geni polimeraz zincir reaksiyonu (PZR) ile çoğaltılmıştır. Daha sonra, PCR örnekleri dizin analizine tabi tutulmuştur. Dizin analizi yapılan örneklerde öncelikle SCD1 geni 5. ekzonda yer alan 3 adet tek nükleotid polimorfizmi (SNP), sırasıyla, A702G, T762C ve C878T, tespit edilmiştir. Bunlardan özellikle C878T SNP sonucuna bakılarak bireyin A (293Ala) ve V (293 Val) protein varyantından hangisi olduğu belirlenmiştir. Çalışmamızda hem GAK hem de DAK ırkı sığırlarda A varyantı ve AA genotipi sırasıyla 0,91, 0,71 ve 0,43, 0,29 olarak belirlenmiştir. Özellikle GAK ırkı sığırlarda atasal bir allel olduğu ileri sürülen V allel frekansı oldukça düşüktür. Her iki sığır ırkında da VV genotipe sahip 2 adet birey tespit edilmiştir. GAK ve DAK ırkı sığırlara yüksek düzeyde AA genotipine ve A alleline sahip olmaları süt ve et yağ içeriği açısından büyük bir avantaj sağlamaktadır.

Kaynakça

  • Akıs, A., Oztabak, K., 2013. Bos indicus associated alleles in Anatolian cattle breeds support zebu introgression into Near East. Journal of Biological Research-Thessaloniki 19, 131-138.
  • Corl, B.A., Baumgard, L.H., Dwyer, D.A., Griinari, J.M., Phillips, B.S., Bauman, D.E., 2001. The role of Δ9-desaturase in the production of cis-9, trans-11 CLA. Journal of Nutritional Biochemistry 12, 622-630.
  • Edwards, C.J., Baird, J.F., Machugh, D.E., 2007. Taurine and Zebu admixture in Near Eastern cattle: A comparison of mitochondrial, autosomal and Y-chromosomal data. Animal Genetics 38, 520-524.
  • Grummer, R.R., 1991. Effect of feed on composition of milk fat. Journal of Dairy Science 74, 3244-3257.
  • Islas-Trejo, A., Fragment Johnson, A., Medrano, F.J., 2002. Genomic structure and expression of bovine stearoyl- CoA desaturase gene. In: Proceeding of Plant, Animal and Microbe Genomes Conference, San Diego, CA., 766.
  • Kgwatalala, P.M., Ibeagha-Awemu, E.M., Mustafa, A.F., Zhao, X., 2009. Influence of stearoyl-coenzyme A desaturase 1 genotype and stage of lactation on fatty acid composition of Canadian Jersey cows. Journal of Dairy Science 92, 1220-1228.
  • Kromhout, D., Menotti, A., Kestleloot, H., Sans, S., 2002. Prevention of coronary heart disease by diet and lifestyle: Evidence from prospective cross-cultural, cohort and interventional studies. Circulation 105, 893- 898.
  • Macciota, N.P.P., Mele, M., Conte, G., Serra, A., Cassandro, M., Dal Zotto, R., Borlino, A.C., Pagnacco, G., Secchiari, P., 2008. Association between an polymorphism at the stearoyl CoA desaturase locus and milk production traits in Italian Holsteins. Journal of Dairy Science 91, 3184-3189.
  • Matsuhashi, T., Maruyama, S., Uemoto, Y., Kobayashi, N., Mannen, H., Abe T., Sakaguchi, S., Kobayashi, E., 2011. Effects of bovine fatty acid synthase, stearoyl- coenzyme A desaturase, sterol regulatory element- binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle. Journal of Dairy Science 89, 12-22.
  • Medrano, J.F., Johnson, A., Depeters, E.J., Islas A., 1999. Genetic modification of composition of milk fat: Identification of polymorphisms within the bovine stearoyl-CoA- desaturase gene. Journal of Dairy Science 82, 71-75.
  • Mele, M., Conte, G., Castiglioni, B., Chessa, S., Macciotta, N.P.P., Sera, A., Buccioni, A., Pagnacco, G., Secchiari, P., 2007. Stearoyl-coenzyme A desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. Journal of Dairy Science 90, 4458- 4465.
  • Milanesi, E., Nicoloso, L., Crepaldi, P., 2008. Stearoyl CoA desaturase (SCD) gene polymorphisms in Italian cattle breeds. Journal of Animal Breeding and Genetics 125, 63-67.
  • Miller, M., Dykes, D.D., Polesky, H.F., 1985. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16, 1215-1219.
  • Moioli, B., Contarini, G., Avalli, A., Catillo, G., Orru, L., De Matteis, G., Masoero, G., Napolitano, F., 2007. Effect of stearoyl-coenzyme A desaturase polymorphism on fatty acid composition of milk. Journal of Dairy Science 90, 3553-3558.
  • Ohsaki, H., Tanaka, A., Hoashi, S., Sasazaki, S., Oyama, K., Taniguchi, M., Mukai, F., Mannen, H., 2009. Effects of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herd. Animal Science Journal 80, 225-232.
  • Schennink, A., Heck, J.M.L., Bovenhuis, H., Visker M.H.P.W., Van Valenberg, H.J.F., Van Aredonk, J.A.M., 2008. Milk fatty acid unsaturation : genetic parameters and effects of stearoyl-coenzyme A desaturase (SCD1) and acyl Co A: Diacylglycerol acyltransferase (DGAT1). Journal of Dairy Science 91, 2135-2143.
  • Taniguchi, M., Utsugi, T., Oyama, K., Manen, H., Kobayashi, M., Tanabe, Y., Ogino, A., Tsuji, S., 2004. Genotype of stearoyl-coenzyme A desaturase is associated with fatty acid composition in Japanese Black cattle. Mammalian Genome 14, 142-148.
  • Yeh, F., Yang, R.C., Boyle, T., 2000. Popgene (v.1.32) Microsoft Windows-based freeware for Population Genetic Analysis. http://www.ualberta.ca/~fyeh/ Pop32.exe. (Erişim 01.10.2011). from
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

İjlal Paya Bu kişi benim

Kemal Öztabak

Yayımlanma Tarihi 1 Temmuz 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 41 Sayı: 2

Kaynak Göster

APA Paya, İ., & Öztabak, K. (2015). Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 41(2), 218-222. https://doi.org/10.16988/iuvfd.2015.15635
AMA Paya İ, Öztabak K. Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle. iuvfd. Ağustos 2015;41(2):218-222. doi:10.16988/iuvfd.2015.15635
Chicago Paya, İjlal, ve Kemal Öztabak. “Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 41, sy. 2 (Ağustos 2015): 218-22. https://doi.org/10.16988/iuvfd.2015.15635.
EndNote Paya İ, Öztabak K (01 Ağustos 2015) Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 41 2 218–222.
IEEE İ. Paya ve K. Öztabak, “Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle”, iuvfd, c. 41, sy. 2, ss. 218–222, 2015, doi: 10.16988/iuvfd.2015.15635.
ISNAD Paya, İjlal - Öztabak, Kemal. “Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 41/2 (Ağustos 2015), 218-222. https://doi.org/10.16988/iuvfd.2015.15635.
JAMA Paya İ, Öztabak K. Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle. iuvfd. 2015;41:218–222.
MLA Paya, İjlal ve Kemal Öztabak. “Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, c. 41, sy. 2, 2015, ss. 218-22, doi:10.16988/iuvfd.2015.15635.
Vancouver Paya İ, Öztabak K. Determination of Stearoyl-Coenzyme A Desaturase 1 Gene Variants in South Anatolian Red and East Anatolian Red Cattle. iuvfd. 2015;41(2):218-22.