The distribution of Rotavirus G and P genotypes in children with acute gastroenteritis in Cukurova region, Turkey

Yıl 2016, Cilt: 1 Sayı: 1, 3 - 9, 30.06.2016

Fugen Yarkın

https://doi.org/10.5455/jicm.7.20160520

Cited By: 1

Öz

Background: Rotavirus
is the major cause of acute gastroenteritis in infants and young children
worldwide. The aim of the study was to determine the frequency of rotavirus
infection and the distribution of rotavirus G and P genotype combination among
children under 5 years of age with acute gastroenteritis in Cukurova region,
Turkey, between October 2009 and June 2010.

Material and Methods: The stool specimens (n=846) collected from children with
acute gastroenteritis were analyzed by enzyme-linked immunosorbent assay
(ELISA) for group A rotavirus antigen. Semi-nested multiplex reverse
transcription-polymerase chain reaction (RT-PCR) test was performed for
rotavirus G and P genotyping. 

Results: The
rate of rotavirus infection was found to be in 144 patients (17%). The
predominant rotavirus genotype was G1P[8] (22.2%), followed by G1P[4] (17.3%),
G2P[4] (13.8%), G9P[4] (6.3%), G9P[8] (4.8%), G2P[8] (2.8%), G1P[10] (2.1%) and
G4P[8] (1.4%). The most common G genotype was G1 (41.7%), followed by G2 (16.6%),
G9 (11.1%) and G4 (1.4%). Rotavirus P[4] genotype was identified in 37.5%, P[8]
in 31.2% and P[10] in 2.1% of samples. The prevalence of mixed rotavirus
infections was 29.2% (n=42).

Conclusion: Although
the predominant rotavirus genotypes circulating during the study period in our
region are targets of current rotavirus vaccines, uncommon, non-vaccine
rotavirus genotype combinations such as G1P[4] 
and G9P[4], which might appear to be the result of mixed rotavirus
infections with high rate (29.2%), were also detected. G1 is included in both
recent rotavirus vaccines. The continuous investigation of molecular
epidemiology of rotavirus infections is essential to evaluate the effectiveness
of rotavirus vaccines.

Anahtar Kelimeler

Rotavirus, Gastroenteritis, Genotypes, ELISA, RT-PCR

Kaynakça

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