Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking

Yıl 2018, Cilt: 10 Sayı: 3, 289 - 293, 23.10.2018

Kabil Shermatov Halil Kazanasmaz Bulent Guzel Nazime Cebi

https://doi.org/10.18521/ktd.435349

Öz

Background: DNA damage associated with passive
smoking has been shown in children. Severe DNA damage can change the apoptosis
process of the cell.  M30 and M65
biomarkers are markers showing the apoptosis process and can be quantitatively
measured. We aimed to determine M30 and M65 levels as apoptosis biomarkers in
children exposed to passive smoking.

Methods: A total of 79 children were included
in the study. Urine cotinine level was measured with the chemiluminescence
method and the levels of apoptosis biomarkers M30 and M65 were measured in
peripheral venous blood with PEVIVA/ALEXIS ELISA kits.

Results: The mean serum M30 level of the group
exposed to smoking was found to be significantly low compared to that of the
control group (p=0.01). The serum M65 value was not significant in either group
(p=0.1). No correlation was determined between the mean cotinine level and M30
(r=0.25, p=0.02) or M65 (r=0.16, p=0.15).

Conclusion: The results of this study showed that
the apoptosis biomarker M30 levels of the children exposed to passive smoking
were low compared to those of the control group which can be interpreted as
apoptosis inhibition in exposure to cigarette smoke. Further clinical studies
are needed.

Anahtar Kelimeler

Apoptosis, M30, M65, cotinine, nicotine

Pasif Sigara İçen Çocuklarda Apotozis Biyobelirteci Olarak Serum M30 ve M65 Protein Düzeyleri

Öz

Amaç: Pasif sigara içiciliğine bağlı DNA
hasarı çocuklarda gösterilmiştir. Ciddi DNA hasarı, hücrenin apoptoz sürecini
değiştirebilir. M30 ve M65 biyobelirteçleri apoptoz sürecini gösteren
belirteçlerdir ve kantitatif olarak ölçülebilir. Pasif sigara içiciliğine maruz
kalan çocuklarda M30 ve M65 düzeylerini apoptoz biyobelirteçleri olarak
belirlemeyi amaçladık.

Yöntemler: Çalışmaya toplam 79 çocuk dahil
edildi. İdrar kotinin düzeyi kemilüminesans yöntemiyle ölçüldü ve periferik
venöz kanda apoptoz biyobelirteçleri olan M30 ve M65 düzeyleri PEVIVA / ALEXIS
ELISA kitleri ile ölçüldü.

Bulgular: Sigaraya maruz kalan grubun ortalama
M30 düzeyi kontrol grubuna göre anlamlı derecede düşük bulundu (p = 0.01).
Serum M65 değeri her iki grupta da anlamlı değildi (p = 0.1). Ortalama kotinin
düzeyi ile M30 (r = 0.25, p = 0.02) veya M65 (r = 0.16, p = 0.15) arasında
korelasyon saptanmadı.

Sonuç: Bu çalışmanın sonuçları, pasif
sigara içiciliğine maruz kalan çocukların apoptoz biyobelirteçi olan M30
düzeylerinin, kontrol grubuna göre kıyaslandığında sigara dumanına maruziyette
apoptoz inhibisyonu olarak yorumlanabilecek şekilde düşük olduğunu gösterdi.
İleri klinik çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

Apoptoz, M30, M65, cotinine, nicotine

Kaynakça

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