Ergene Nehir Suyunun Soğan (Allium cepa L.) Kök Ucu Hücrelerinde Sitogenetik Etkisinin Değerlendirilmesi

Yıl 2017, Cilt: 29 Sayı: 3, 111 - 117, 01.11.2017

Martin Kanev Kezban Yıldırım Fulya Dilek Gökalp

https://doi.org/10.7240/marufbd.311079

Öz

Türkiye’de
endüstriyel hareketlerin artması ile birlikte, Trakya bölgesinde organize
sanayi alanları kurulmuş ve bunun sonucunda özellikle Trakya bölgesini besleyen
Ergene Nehri ve çevre alanlarında kirlilik artmıştır. Bu çalışmada, Ergene
nehir suyunun Allium cepa L. kök ucu
hücrelerinde genotoksik ve sitotoksik etkilerinin araştırılması amaçlanmıştır.
Çalışmada Ergene nehir suyundan iki farklı istasyondan aynı dönemde alınan su
örnekleri 0.1/100 mL, 1/100 mL, 5/100 mL, 10/100 mL, 20/100 mL ve 40/100 mL konsantrasyonlar
ile soğan (Allium cepa L.) kök ucu
hücreleri 24 saat muamele edilerek sitogenetik anormallikler ve mitotik indeks
saptanmıştır. Her iki istasyondan alınan örneklerin, uygulanan tüm dozlarında
mitotik anormallikleri kontrole göre anlamlı bir şekilde arttırdığı ve mitotik
indeksi anlamlı olarak değiştirdiği belirlenmiştir. En fazla gözlenen mitotik
anormallikler kromozomlarda asenkronizasyon, yanlış kutuplaşma ve C-mitozdur.
Sonuçlar Ergene nehir suyunun A. cepa
testinde genotoksik ve sitotoksik etkili olduğunu göstermektedir.

Anahtar Kelimeler

Ergene Nehri, Allium cepa, sitotoksik, genotoksik

Evaluation Of Cytogenetic Effects Of Ergene River Water On Onion (Allium cepa L.) Root Tip Cell

Öz

As a result of industrialization movement in Turkey,
organized industrial zone have been 
established in Thrace, and especially pollution reached significant size
in there and their surrounding area. In the present study, it was aimed to
study the genotoxic and cytotoxic effects of the Ergene River water on Allium cepa root tip cells. In the
study, Ergene river water samples taken at the same time from two different
stations. Samples were exposed with 0.1/100 mL, 1/100 mL, 5/100 mL, 10/100 mL,
20/100 mL, and 40/100 mL doses for 24 hours on onion (Allium cepa L.) root tip cells, and cytogenetic abnormalities and
mitotoic index were investigated. On applied all doses of both stations,
mitotic abnormalities were significantly increased compared with control, and
mitotic index was significantly changed. The most observed mitotic
abnormalities in chromosomes are asencronization, incorrect polarization, and
C-mitosis. The results indicated that the Ergene River has genotoxic, and
cytotoxic effects on plants.

Anahtar Kelimeler

The Ergene River, Allium cepa, cytotoxic, genotoxic

Kaynakça

• [1] Barberio, A., Barros, L., Voltolini, J. C., & Mello, M. L. (2009). Evaluation of the cytotoxic and genotoxic potential of water from the River Paraiba do Sul, in Brazil, with the Allium cepa L. test. Braz J Biol, 69(3), 837-842.
• [2] Kohlpoth, M., Rusche, B., & Nusse, M. (1999). Flow cytometric measurement of micronuclei induced in a permanent fish cell line as a possible screening test for the genotoxicity of industrial waste waters. Mutagenesis, 14(4), 397-402.
• [3] Kocaman, H., Koldere Akın, Y., & Oğuzhan, A. (2011). Trakya’da Ergene Nehri kirliliğinin tarım üretimine olan etkisi: Edirne örneği. Karadeniz Fen Bilimleri Dergisi, 2(5), 89-104.
• [4] Ordu, Ş., & Demir, A. (2007). Determınation of water quality of Ergene River by planning envıronmental informatİon system. Sigma Journal of Engineering and Natural Sciences, 25(1), 101.
• [5] Mehdi, B., Lehner, B., Gombault, C., Michaud, A., Beaudin, I., Sottile, M. F., & Blondlot, A. (2015). Simulated impacts of climate change and agricultural land use change on surface water quality with and without adaptation management strategies. Agriculture Ecosystems & Environment, 213, 47-60.
• [6] Chandra, S., Chauhan, L. K., Murthy, R. C., Saxena, P. N., Pande, P. N., & Gupta, S. K. (2005). Comparative biomonitoring of leachates from hazardous solid waste of two industries using Allium test. Sci Total Environ, 347(1-3), 46-52. doi: 10.1016/j.scitotenv.2005.01.002
• [7] Athanasio, C. G., Pra, D., & Rieger, A. (2014). Water quality of urban streams: the Allium cepa seeds/seedlings test as a tool for surface water monitoring. ScientificWorldJournal, 2014, 391367. doi: 10.1155/2014/391367
• [8] Ozkara, A., Akyil, D., Eren, Y., Erdogmus, S. F., Konuk, M., & Saglam, E. (2015). Assessment of cytotoxic and genotoxic potential of pyracarbolid by Allium test and micronucleus assay. Drug Chem Toxicol, 38(3), 337-341. doi: 10.3109/01480545.2014.966831
• [9] Yönetmelik. (2015). Yüzeysel su kalitesi yönetimi yönetmeliğinde değişiklik yapılmasına yönelik dair yönetmelik. Resmi Gazete: Orman ve Su İşleri Bakanlığı.
• [10] Alberti, G., D'Agostino, G., Palazzo, G., Biesuz, R., & Pesavento, M. (2005). Aluminium speciation in natural water by sorption on a complexing resin. J Inorg Biochem, 99(9), 1779-1787. doi: 10.1016/j.jinorgbio.2005.06.017
• [11] Sinha, R., Mathur, S., & Brighu, U. (2015). Aluminium removal from water after defluoridation with the electrocoagulation process. Environ Technol, 36(21), 2724-2731. doi: 10.1080/09593330.2015.1043958
• [12] Britt, R. D., Suess, D. L., & Stich, T. A. (2015). An Mn(V)-oxo role in splitting water? Proc Natl Acad Sci U S A, 112(17), 5265-5266. doi: 10.1073/pnas.1505223112
• [13] Laughinghouse, H. D. t., Pra, D., Silva-Stenico, M. E., Rieger, A., Frescura, V. D., Fiore, M. F., & Tedesco, S. B. (2012). Biomonitoring genotoxicity and cytotoxicity of Microcystis aeruginosa (Chroococcales, cyanobacteria) using the Allium cepa test. Sci Total Environ, 432, 180-188. doi: 10.1016/j.scitotenv.2012.05.093
• [14] Türkoğlu, Ş., & Koca, S. (1999). The effects of paraquat (gramoxone) on mitotic division, chromosomes and DNA amount in Vicia faba L. C.Ü.Fen-Edebiyat Fak. Fen Bilimleri Dergisi, 21.
• [15] Wang, J. J., Cheng, W. X., Ding, W., & Zhao, Z.-M. (2006). The effect of the insecticide dichlorvos on esterase activity extracted from the psocids, Liposcelis bostrychophila and L. entomophila. Journal of Insect Science, 4(23), 5.
• [16] Rank, J., & Nielsen, M. H. (1998). Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay. Mutat Res, 418(2-3), 113-119.
• [17] Monarca, S., Feretti, D., Collivignarelli, C., Guzzella, L., Zerbini, I., Bertanza, G., & Pedrazzani, R. (2000). The influence of different disinfectants on mutagenicity and toxicity of urban wastewater. Water Research, 17, 4261-4269.
• [18] Bekoğlu Yılmaz, D. (2013). Ergene Nehrİ Suyunun Secale cereale L. Tohumunun Çİmlenmesİ ve Kök Ucu Mİtoz Bölünme Üzerİne Etkİsİ. Trakya Ünİversİtesİ Fen Bİlİmlerİ Enstİtüsü.
• [19] Evseeva, T. I., Geras'kin, S. A., & Shuktomova, II. (2003). Genotoxicity and toxicity assay of water sampled from a radium production industry storage cell territory by means of Allium-test. J Environ Radioact, 68(3), 235-248. doi: 10.1016/S0265-931X(03)00054-7
• [20] Şahin, Z. (2008). Büyük Menderes Nehri ile Sulanan Aydın Bölgesi'ndeki Toprakların Genotoksisitesinin Allium Test Sistemi ile Belirlenmesi. Adnan Menderes Üniversitesi Fen Bilimleri Enstitüsü.
• [21] Kong, Z. M., Zang, Y., & Wu, Q. L. (1998). Monitoring the genotoxicity of Lake Taihu, using two kinds of micronucleus tests. Environ Pollut, 99(2), 279-283.
• [22] Kovalchuk, O., Kovalchuk, I., Arkhipov, A., Telyuk, P., Hohn, B., & Kovalchuk, L. (1998). The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by the Chernobyl accident. Mutat Res, 415(1-2), 47-57.
• [23] Bolle, P., Mastrangelo, S., Tucci, P., & Evandri, M. G. (2004). Clastogenicity of atrazine assessed with the Allium cepa test. Environ Mol Mutagen, 43(2), 137-141. doi: 10.1002/em.20007