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Kriyopreservasyon oligoastenoteratozoospermik erkeklerde DNA fragmantasyonunu ve ultrastrüktürel hasarı tetikler

Yıl 2017, Cilt: 30 Sayı: 2, 63 - 72, 21.05.2017
https://doi.org/10.5472/marumj.344797

Öz

Amaç: Oligoastenoteratozoospermi (OAT) erkek infertilitisi

sebeplerinden biridir. Kriyopreservasyon infertilite yönetimi

için değerlidir. Bu çalışmanın amacı, OAT’lı hastalarda

sperm motilitesi, morfolojisi, ultrastrüktürel detaylar ve DNA

fragmentasyonu üzerine kriyopreservasyonun etkilerini ortaya

çıkarmaktır.

Gereç ve Yöntem: Bu gözlemsel çalışmada, gönüllü 20

normospermik ve 20 OAT’lı hastadan ejakulatlar toplanmıştır.

Ejakulatlar, -196oC sıvı nitrojende saklanmış ve çözdürmeden üç

ve altı ay sonra analiz edilmiştir. Ejakulatlarda, motilite, morfoloji

ve DNA fragmantasyonu dondurma öncesi ve sonrasında

değerlendirilmiştir.

Bulgular: Her iki grupta da sperm motilitesi ve morfolojisi

kriyopresvasyondan etkilenmiştir. Her iki grupta da çözdürmeden

sonra morfolojik değişiklikler anlamlı artmıştır. Ultrastrüktürel

incelemeler membran bütünlüğünün değiştiğini ve akrozomal

hasarın arttığını göstermiştir. Her iki grupta da, dondurma öncesi

ile karşılaştırıldığında, çözdürme sonrasında DNA kırıkları

olan hücrelerin arttığı gözlenmiştir. Her iki dondurma-çözme

periyodunda, kontrol grubu ile kıyaslandığında OAT grubunda

DNA kırıkları olan spermatozoa sayısının anlamlı olarak daha

yüksek olduğu gözlenmiştir.

Sonuç: Kriyopreservasyon, hem normospermik hem de OAT

gruplarında ultrastrüktürel hasar yapmaktadır ve DNA hasarını

indüklemektedir. Bu hasar OAT hastalarında daha ciddidir.

Kaynakça

  • Oehninger S, Duru NK, Srisombu C, Morshedi M. Assessment of sperm cryodamage and strategies to improve outcome. Mol Cell Endocrinol 2000; 169:3-10. doi: 10.1016/ S0303-7207(00)00343-9
  • Anger JT, Gilbert BR, Golstein M. Cryopreservation of sperm: indications, methods and results. J Urol 2003; 170(4 Pt1):1079-84. doi: 10.1097/01.ju.0000084820.98430.b8
  • Glander HJ, Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod 1999;5:109-15. doi: 10.1093/molehr/5.2.109
  • Schiller J, Arnhold J, Glander HJ, Arnold K. Lipid analysis of human spermatozoa and seminal plasma by MALDITOF mass spectrometry and NMR spectroscopy - effects of freezing and thawing. Chem Phys Lipids 2000; 106: 145-56. doi: 10.1016/S0009-3084(00)00148-1
  • Liu CH, Tsao HM, Cheng TC, et al. DNA fragmentation, mitochondrial dysfunction and chromosomal aneuploidy in the spermatozoa of oligoasthenoteratozoospermic males. J Asist Reprod Genet 2004; 21: 119-26. doi: 10.1023/B:JARG.0000029495.22787.83
  • Kalthur G, Adiga SK, Upadhya S, Rao S, Kumar P. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil Steril 2008; 89: 1723–7. doi: 10.1016/j. fertnstert.2007.06.087
  • World Health Organisation. WHO laboratory manual for the examination and processing of human semen, 5th ed. Geneva, Switzerland: WHO Press, 2010.
  • Aydin MS, Senturk GE, Ercan F. Cryopreservation increases DNA fragmentation in spermatozoa of smokers. Acta Histochem 2013; 115: 394-400. doi: 10.1016/j. acthis.2012.10.003
  • Stanic P, Tandara M, Sonicki Z, Simunic V, Radakovic B, Suchanek E. Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gyn Reprod Biol 2000; 91: 65-70. doi: 10.1016/ S0301-2115(99)00255-9
  • Zeron Y, Pearl M, Borochow A, Arav A. Kinetic and temporal factors influence chilling injury to germinal vesicle mature bovine oocytes. Cryobiology 1999; 38: 35-42. doi: 10.1006/ cryo.1998.2139
  • Giraud MN, Motta C, Boucher D, Grizard G. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Human Reprod 2000; 15: 2160-4. doi: 10.1093/ humrep/15.10.2160
  • Quinn PJ. A lipid-phase separation model of low-temperature damage to biological membranes. Cryobiology 1985; 22: 128-46. doi: 10.1016/0011-2240(85)90167-1
  • O’Connell M, McClure N, Lewis SE. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Human Reprod 2002; 17: 704-9. doi: 10.1093/humrep/17.3.704
  • Said TM, Gaglani A, Agarwal A. Implication of apoptosis in sperm cryoinjury. Reprod BioMed Online 2010; 21: 456-62. doi: 10.1016/j.rbmo.2010.05.011
  • Saleh RA, Agarwal A. Oxidative stress and male infertility: from research bench to clinical practice. J Androl 2002; 23: 737-52.
  • Lasso JL, Noiles EE, Alvarez JG, Storey BT. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl 1994; 15: 255-65.
  • Gadea J, Molla M, Selles E, Marco MA, Garcia-Vazquez FA, Gardon JC. Reduced glutathione content in human sperm is decreased after cryopreservation: effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology 2011; 62: 40-6. doi: 10.1016/j. cryobiol.2010.12.001
  • Di Santo M, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: Update on techniques, effect on DNA integrity, and implications for ART. Adv Urol 2012; 2012: 854837. doi: 10.1155/2012/854837
  • Martínez-Soto JC, Landeras J, Gadea J. Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success. Andrology 2013; 1: 365-75. doi: 10.1111/j.2047- 2927.2012.00040.x
  • Check ML, Check JH, Long R. Detrimental effects of cryopreservation on structural and functional integrity of the sperm membrane. Arch Androl 1991; 27: 155-60.
  • Paasch U, Sharma RK, Grupta AK, et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Bio Reprod 2004; 71: 1828-37. doi: 10.1095/ biolreprod.103.025627
  • Zribi N, Chakroun NF, Euch HE, Gargouri J, Bahloul A, Keskes LA. Effects of cryopreservation on human sperm deoxyribonucleic acid integrity. Fertil Steril 2010; 93: 159- 66. doi: 10.1016/j.fertnstert.2008.09.038
  • Nogueira D, Bourgain C, Verheyen G, Van Steirteghem AC. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies. Hum Reprod 1999; 14: 2041-9. doi: 10.1093/humrep/14.8.2041
  • Ozkavukcu S, Erdemli E, Işık A, Oztuna D, Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet 2008; 25: 403-11. doi: 10.1007/s10815-008- 9232-3
  • Evenson DP, Jost LK, Marshall D, et al. Utility of the sperm chromatin structure assay as a diagnostic andrognostic tool in the human fertility clinic. Hum Reprod 1999; 14: 1039-49. doi: 10.1093/humrep/14.4.1039
  • Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-43. doi: 10.1016/S0015- 0282(02)004948-8
  • Aitken RJ, De Luliis GN, McLachlan RI. Biological and clinical significance of DNA damage in the male germ line. Int J Androl 2009; 32: 46-56. doi: 10.1111/j.1365- 2605.2008.00943.x
  • Desai N, Sharma R, Makker K, Sabanegh E, Agarwal A. Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men. Fertil Steril 2009; 92: 1626- 31. doi: 10.1016/j.fertnstert.2008.08.109
  • Brugnon F, Ouchchane L, Pons-Rejraji H, Artonne C, Farigoule M, Janny L. Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia. Hum Reprod 2013; 28: 2045- 57. doi: 10.1093/humrep/det253

Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men

Yıl 2017, Cilt: 30 Sayı: 2, 63 - 72, 21.05.2017
https://doi.org/10.5472/marumj.344797

Öz

Objective: Oligoasthenoteratozoospermia (OAT) is one of the

causes of male infertility. Cryopreservation is valuable for the

management of infertility. This study aimed to reveal the effects

of cryopreservation on sperm motility, morphology, ultrastructural

details and DNA fragmentation in patients with OAT.

Materials and Methods: In this observational study,

ejaculates were collected from 40 male volunteers of whom 20

were OAT and 20 were normospermic. The ejaculates were stored

in liquid nitrogen at -196oC and analysed following thawing after

1 or 3 months. Ejaculates were evaluated in terms of motility,

morphology and DNA fragmentation before and after thawing.

Results: Sperm motility and morphology were both affected

by cryopreservation in samples from both groups. After thawing,

spermatozoa with morphological abnormalities were increased

significantly in both groups. Ultrastructural investigations showed

alteration in integrity of the membranes and increased acrosomal

defects. Post-thaw investigations revealed prominent increases

in the number of DNA fragmented cells in both groups when

compared to the results before freezing. OAT groups revealed a

significantly higher number of DNA fragmented spermatozoa

compared to the normospermic group for both time periods.

Conclusion: Cryopreservation produces ultrastructural

damage and induces DNA fragmentation in both normospermic

and OAT groups. The damage is more severe in the OAT group.

Kaynakça

  • Oehninger S, Duru NK, Srisombu C, Morshedi M. Assessment of sperm cryodamage and strategies to improve outcome. Mol Cell Endocrinol 2000; 169:3-10. doi: 10.1016/ S0303-7207(00)00343-9
  • Anger JT, Gilbert BR, Golstein M. Cryopreservation of sperm: indications, methods and results. J Urol 2003; 170(4 Pt1):1079-84. doi: 10.1097/01.ju.0000084820.98430.b8
  • Glander HJ, Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod 1999;5:109-15. doi: 10.1093/molehr/5.2.109
  • Schiller J, Arnhold J, Glander HJ, Arnold K. Lipid analysis of human spermatozoa and seminal plasma by MALDITOF mass spectrometry and NMR spectroscopy - effects of freezing and thawing. Chem Phys Lipids 2000; 106: 145-56. doi: 10.1016/S0009-3084(00)00148-1
  • Liu CH, Tsao HM, Cheng TC, et al. DNA fragmentation, mitochondrial dysfunction and chromosomal aneuploidy in the spermatozoa of oligoasthenoteratozoospermic males. J Asist Reprod Genet 2004; 21: 119-26. doi: 10.1023/B:JARG.0000029495.22787.83
  • Kalthur G, Adiga SK, Upadhya S, Rao S, Kumar P. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil Steril 2008; 89: 1723–7. doi: 10.1016/j. fertnstert.2007.06.087
  • World Health Organisation. WHO laboratory manual for the examination and processing of human semen, 5th ed. Geneva, Switzerland: WHO Press, 2010.
  • Aydin MS, Senturk GE, Ercan F. Cryopreservation increases DNA fragmentation in spermatozoa of smokers. Acta Histochem 2013; 115: 394-400. doi: 10.1016/j. acthis.2012.10.003
  • Stanic P, Tandara M, Sonicki Z, Simunic V, Radakovic B, Suchanek E. Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gyn Reprod Biol 2000; 91: 65-70. doi: 10.1016/ S0301-2115(99)00255-9
  • Zeron Y, Pearl M, Borochow A, Arav A. Kinetic and temporal factors influence chilling injury to germinal vesicle mature bovine oocytes. Cryobiology 1999; 38: 35-42. doi: 10.1006/ cryo.1998.2139
  • Giraud MN, Motta C, Boucher D, Grizard G. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Human Reprod 2000; 15: 2160-4. doi: 10.1093/ humrep/15.10.2160
  • Quinn PJ. A lipid-phase separation model of low-temperature damage to biological membranes. Cryobiology 1985; 22: 128-46. doi: 10.1016/0011-2240(85)90167-1
  • O’Connell M, McClure N, Lewis SE. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Human Reprod 2002; 17: 704-9. doi: 10.1093/humrep/17.3.704
  • Said TM, Gaglani A, Agarwal A. Implication of apoptosis in sperm cryoinjury. Reprod BioMed Online 2010; 21: 456-62. doi: 10.1016/j.rbmo.2010.05.011
  • Saleh RA, Agarwal A. Oxidative stress and male infertility: from research bench to clinical practice. J Androl 2002; 23: 737-52.
  • Lasso JL, Noiles EE, Alvarez JG, Storey BT. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl 1994; 15: 255-65.
  • Gadea J, Molla M, Selles E, Marco MA, Garcia-Vazquez FA, Gardon JC. Reduced glutathione content in human sperm is decreased after cryopreservation: effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology 2011; 62: 40-6. doi: 10.1016/j. cryobiol.2010.12.001
  • Di Santo M, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: Update on techniques, effect on DNA integrity, and implications for ART. Adv Urol 2012; 2012: 854837. doi: 10.1155/2012/854837
  • Martínez-Soto JC, Landeras J, Gadea J. Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success. Andrology 2013; 1: 365-75. doi: 10.1111/j.2047- 2927.2012.00040.x
  • Check ML, Check JH, Long R. Detrimental effects of cryopreservation on structural and functional integrity of the sperm membrane. Arch Androl 1991; 27: 155-60.
  • Paasch U, Sharma RK, Grupta AK, et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Bio Reprod 2004; 71: 1828-37. doi: 10.1095/ biolreprod.103.025627
  • Zribi N, Chakroun NF, Euch HE, Gargouri J, Bahloul A, Keskes LA. Effects of cryopreservation on human sperm deoxyribonucleic acid integrity. Fertil Steril 2010; 93: 159- 66. doi: 10.1016/j.fertnstert.2008.09.038
  • Nogueira D, Bourgain C, Verheyen G, Van Steirteghem AC. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies. Hum Reprod 1999; 14: 2041-9. doi: 10.1093/humrep/14.8.2041
  • Ozkavukcu S, Erdemli E, Işık A, Oztuna D, Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet 2008; 25: 403-11. doi: 10.1007/s10815-008- 9232-3
  • Evenson DP, Jost LK, Marshall D, et al. Utility of the sperm chromatin structure assay as a diagnostic andrognostic tool in the human fertility clinic. Hum Reprod 1999; 14: 1039-49. doi: 10.1093/humrep/14.4.1039
  • Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-43. doi: 10.1016/S0015- 0282(02)004948-8
  • Aitken RJ, De Luliis GN, McLachlan RI. Biological and clinical significance of DNA damage in the male germ line. Int J Androl 2009; 32: 46-56. doi: 10.1111/j.1365- 2605.2008.00943.x
  • Desai N, Sharma R, Makker K, Sabanegh E, Agarwal A. Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men. Fertil Steril 2009; 92: 1626- 31. doi: 10.1016/j.fertnstert.2008.08.109
  • Brugnon F, Ouchchane L, Pons-Rejraji H, Artonne C, Farigoule M, Janny L. Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia. Hum Reprod 2013; 28: 2045- 57. doi: 10.1093/humrep/det253
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Konular Klinik Tıp Bilimleri
Bölüm Makaleler
Yazarlar

Pınar Turan Bu kişi benim

Gözde Erkanlı Şentürk Bu kişi benim

Feriha Ercan Bu kişi benim

Yayımlanma Tarihi 21 Mayıs 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 30 Sayı: 2

Kaynak Göster

APA Turan, P., Erkanlı Şentürk, G., & Ercan, F. (2017). Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men. Marmara Medical Journal, 30(2), 63-72. https://doi.org/10.5472/marumj.344797
AMA Turan P, Erkanlı Şentürk G, Ercan F. Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men. Marmara Med J. Mayıs 2017;30(2):63-72. doi:10.5472/marumj.344797
Chicago Turan, Pınar, Gözde Erkanlı Şentürk, ve Feriha Ercan. “Cryopreservation Triggers DNA Fragmentation and Ultrastructural Damage in Spermatozoa of Oligoasthenoteratozoospermic Men”. Marmara Medical Journal 30, sy. 2 (Mayıs 2017): 63-72. https://doi.org/10.5472/marumj.344797.
EndNote Turan P, Erkanlı Şentürk G, Ercan F (01 Mayıs 2017) Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men. Marmara Medical Journal 30 2 63–72.
IEEE P. Turan, G. Erkanlı Şentürk, ve F. Ercan, “Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men”, Marmara Med J, c. 30, sy. 2, ss. 63–72, 2017, doi: 10.5472/marumj.344797.
ISNAD Turan, Pınar vd. “Cryopreservation Triggers DNA Fragmentation and Ultrastructural Damage in Spermatozoa of Oligoasthenoteratozoospermic Men”. Marmara Medical Journal 30/2 (Mayıs 2017), 63-72. https://doi.org/10.5472/marumj.344797.
JAMA Turan P, Erkanlı Şentürk G, Ercan F. Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men. Marmara Med J. 2017;30:63–72.
MLA Turan, Pınar vd. “Cryopreservation Triggers DNA Fragmentation and Ultrastructural Damage in Spermatozoa of Oligoasthenoteratozoospermic Men”. Marmara Medical Journal, c. 30, sy. 2, 2017, ss. 63-72, doi:10.5472/marumj.344797.
Vancouver Turan P, Erkanlı Şentürk G, Ercan F. Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men. Marmara Med J. 2017;30(2):63-72.