Antidiabetic effects of Salvia triloba and Thymus praecox subsp. skorpilii var. skorpilii in a rat model of streptozotocin/nicotinamide-induced diabetes

Yıl 2017, Cilt: 21 Sayı: 4, 818 - 827, 01.12.2017

Muhammet Emin Çam Sıla Yıldız Büşra Ertaş Ayşe Eda Acar Turgut Taşkın Levent Kabasakal

Öz

Some Salvia and Thymus species of Lamiaceae family come into

prominence with strong antidiabetic effects. Compared to the

other species, there are limited studies on antidiabetic activity

of Salvia triloba (ST) and Thymus praecox subsp. skorpilii var.

skorpilii (TPS). The aim of this study was to adjust the dosage

and to determine the antidiabetic effects of methanol extracts

of ST and TPS in streptozotocin/nicotinamide-induced diabetic

rats. Type II diabetes mellitus (T2DM) was induced by a

single intraperitoneal (i.p.) injection of streptozotocin (STZ)

dissolved in 0.1 M cold citrate buffer (pH 4.5) at a dose of 55

mg/kg/body weight (b.w.) and nicotinamide (100 mg/kg/b.w.)

was given prior to STZ injection. For adjusting dosage, oral

glucose tolerance test (OGTT) was used while insulin tolerance

test (ITT), OGTT, blood glucose levels and animal weights

were used to evaluate the antidiabetic effects of ST and TPS.

According to the OGTT, the most effective doses for ST and

TPS were 200 mg/kg and 100 mg/kg, respectively. At the end

of three weeks, blood glucose levels of control goup reached

to 462.50 mg/dl, whereas ST and TPS-treated groups blood

glucose levels decreased less than 200.00 mg/dl. In conclusion,

the present study suggests that both of ST and TPS methanolic

extracts may be of therapeutic benefit in diabetes and thus need

to further studies.

Anahtar Kelimeler

Type II diabetes mellitus ; Salvia triloba ; Thymus praecox ; Streptozotocin-Nicotinamide ; herbal medicines ; antidiabetic

Kaynakça

• 1. Azaz AD, Irtem HA, Kurkcuoglu M, Baser KHC. Composition and the in vitro antimicrobial activities of the essential oils of some Thymus species. Z Naturforsch 2004; 59c: 75-80. 2. Soleimani Farsani M, Behbahani M, Isfahani HZ. The effect of root, shoot and seed extracts of the Iranian Thymus L. (Family: Lamiaceae) species on HIV-1 replication and CD4 Expression. Cell J 2016; 18: 255-61. 3. Ozen T, Demirtas I, Aksit H. Determination of antioxidant activities of various extracts and essential oil compositions of Thymus praecox subsp. skorpilii var. skorpilii. Food Chem 2011; 124: 58-64. 4. Cvetkovikj I, Stefkov G, Karapandzova M, Kulevanova S. Essential oil composition of Salvia fruticosa Mill. populations from Balkan Peninsula. Macedonian Pharm Bull 2015; 61: 19-26. 5. Yucel E, Guney F, Yucel Sengun I. The wild plants consumed as a food in Mihalıççık district (Eskişehir:Turkey) and consumption forms of these plants. Biol Divers Conserv 2010; 3: 158-75. 6. American Diabetes A. Standards of medical care in diabetes-2016. Diabetes Care 2016; 39: 1-112. 7. American Diabetes A. Diagnosis and classification of diabetes mellitus. Diabetes Care 2012; 35: 64-71. 8. Abdelhafiz AH, Koay L, Sinclair AJ. The effect of frailty should be considered in the management plan of older people with Type 2 diabetes. Future Sci OA 2016; 2: FSO102. 9. Or T, Lm T, Mr P. Type 2 diabetes mellitus in children and adolescents: a relatively new clinical problem within pediatric practice. J Med Life 2016; 9: 235-9. 10. Shewamene Z, Abdelwuhab M, Birhanu Z. Methanolic leaf exctract of Otostegia integrifolia Benth reduces blood glucose levels in diabetic, glucose loaded and normal rodents. BMC Complement Altern Med 2015; 15: 19. 11. Abu-Abeeleh M. A preliminary study of the use of human adipose tissue-derived stem cells for the treatment of streptozotocin-induced diabetes mellitus in a rat model. Future Sci OA 2016; 2: FSO102. 12. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 2001; 50: 537-46. 13. Srinivasan K, Ramarao P. Animal models in type 2 diabetes research: An overview. Indian J Med Res 2007; 125: 451-72. 14. Deeds MC, Anderson JM, Armstrong AS, Gastineau DA, Hiddinga HJ, Jahangir A, Eberhardt JL, Kudva YC. Single dose streptozotocin induced diabetes: Considerations for study design in islet transplantation models. Lab Anim 2011; 45: 131-40. 15. Turkkan A, Savas HB, Yavuz B, Yigit A, Uz E, Bayram NA, Kale B. The prophylactic effect of Viscum album in streptozotocin-induced diabetic rats. North Clin Istanbul 2016; 3: 83-9. 16. Hossain MS, Ahmed M, Islam A. Hypolipidemic and hepatoprotective effect of different fraction of methanolic extract of Momordica charantia (Linn.) in alloxan induced diabetic rats. Int J Pharm Sci Res 2011; 2: 601-7. 17. Groop L. Genetics of the metabolic syndrome. Br J Nutr 2000; 83: 39-48. 18. Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: Diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998; 15: 539-53. 19. Roberts CK, Hevener AL, Barnard RJ. Metabolic syndrome and insulin resistance: Underlying causes and modification by exercise training. Compr Physiol 2013; 3: 1-58. 20. Adam SH, Giribabu N, Rao PV, Sayem AS, Arya A, Panichayupakaranant P, Korla PK, Salleh N. Rhinacanthin C ameliorates hyperglycaemia, hyperlipidemia and pancreatic destruction in streptozotocin-nicotinamide induced adult male diabetic rats. Eur J Pharmacol 2016; 771: 173-90. 21. Emordi JE, Agbaje EO, Oreagba IA, Iribhogbe OI. Antidiabetic and hypolipidemic activities of hydroethanolic root extract of Uvaria chamae in streptozotocin induced diabetic albino rats. BMC Complement Altern Med 2016; 16: 468. 22. Bonora E, Moghetti P, Zancanaro C, Cigolini M, Querena M, Cacciatori V, Corgnati A, Muggeo M. Estimates of in vivo insulin action in man: Comparison of insulin tolerance tests with euglycemic and hyperglycemic glucose clamp studies. J Clin Endocrinol Metab 1989; 68: 374-8. 23. Tovchiga OV. The influence of goutweed (Aegopodium podagraria L.) tincture and metformin on the carbohydrate and lipid metabolism in dexamethasone-treated rats. BMC Complement Altern Med 2016; 16: 235. 24. Korec R. Diabetes diagnosis following intravenous and oral glucose load: animal experiment principles. Z Gesamte Inn Med 1988; 43: 427-9.