Diclofop-methyl: A phenoxy propionate herbicide with multiple toxic effects in mouse embyro fibroblast (NIH/3T3) cell line

Yıl 2017, Cilt: 21 Sayı: 4, 992 - 997, 01.12.2017

Müzeyyen Çeliksöz Bahar Ulus Ezgi Öztaş Gül Özhan

Öz

Diclofop-methyl is a selective post-emergence graminicide from

the phenoxy propionate group of herbicides to be developed

for control of wild oats, millets, and other annual grass weeds.

Diclofop-methyl usage is limited in various grass weed species

due to its toxic effect and exposure risks. However, total annual

usage of is approximately 750.000 pounds in United States,

and more in Asia. Therefore, we aimed to investigate diclofopmethyl’s

toxic potentials in vitro and the following assays were

used; MTT assay for cytotoxicity, comet assay for genotoxicity,

generation of reactive oxygen species (ROS), malondialdehyde

(MDA) and glutathione (GSH) for the potential of oxidative

damage in mouse embryo fibroblast (NIH/3T3) cell line.

Diclofop-methyl was observed to reduce the cell viability

in a concentration manner and the half maximal inhibitory

concentration (IC50) value was 301.7 μM. Diclofop-methyl

caused DNA damage and oxidative stress at the concentrations

between 12.5-400 μM. Tail intensities were at the range of 1.24-

58.21% with increasing concentrations, which are approximately

≤ 1.63-fold of the negative control. Also, MDA levels were

increased ≥ ³11.4-fold of the negative control that denotes lipid

peroxidation was induced. However, there was no significant

increment in the ROS and GSH levels at all concentrations. In

view of the fact that ROS has not been detected, despite its level

of MDA proffers, the idea that oxidative damage may have been

caused by other mechanisms. Our results indicate that diclofopmethyl

was cytotoxic, genotoxic and might have oxidative

damage potential in vitro conditions.

Anahtar Kelimeler

Diclofop-methyl; reactive oxygen species; malondialdehyde; Comet assay

Kaynakça

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